Invitrogen™ BacMam pCMV-Dest Vector

Description

• The BacMam pCMV-Dest Vector combines Gateway™ cloning and BacMam gene expression technologies for easy recombination-based cloning and baculovirus-based expression of a target gene in variety of cell types
• With BacMam technology, a modified insect cell virus (baculovirus) is used as a vehicle to efficiently deliver and transiently express genes in mammalian cells with minimum effort and toxicity
• This technology allows for control over the level of expression through the increase or decrease of viral particle concentration
• When combined with Gateway™ cloning technology, a variety of sizes of target genes can be cloned and expressed
• Sizes ranging from an insert for RNAi to a large gene with introns (38 kb) have been cloned and expressed successfully using the BacMam pCMV-Dest Vector
• This plasmid accommodates various cloning schemes, including Gateway™, Seamless Assembly, and traditional cloning using restriction enzymes
• The BacMam pCMV-Dest Vector contains a CMV promoter to drive constitutive expression of the target gene
• It also contains VSV-G elements, which enable baculovirus to have high transduction efficiency, and a WPRE element, which elongates transient expression in mammalian systems
• To facilitate successful cloning, AmpR and gentamicin have been incorporated for positive selection, as well as Cm(R) (chloramphenicol resistance gene) for negative selection
• Upon successful cloning, the region containing the CmR is replaced by the insert, selecting against any bacteria that still have a CmR-containing region.