NBP229540

Novus Biologicals™ pCL-Eco Retrovirus Packaging Vector

Manufacturer: Novus Biologicals™

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Pack Size SKU Availability Price
Each of 1 NBP229540-Each-of-1 In Stock ₹ 35,592.96

NBP229540 - Each of 1

₹ 35,592.96

In Stock

Quantity

1

Base Price: ₹ 35,592.96

GST (18%): ₹ 6,406.733

Total Price: ₹ 41,999.693

Storage Buffer

10µg in 20µL 1x TE (10mM Tris, pH 7.5, 1mM EDTA)

Content And Storage

Store at -20°C. Avoid freeze-thaw cycles.

Quantity

10 μg

Vector

pCL-Eco Retrovirus Packaging Vector

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Description

  • The pCL-ECO packaging vector is a part of the RetroMax expression system and has been designed to maximize recombinant-retrovirus titers in a simple, efficient, and flexible experimental system
  • By introducing a retroviral vector into a sell expressing retroviral proteins, retroviral particles (virions) are shed into the culture medium at the rate of about 1 infectious particle/cell/day
  • Retrovirus tropism is determined at 3 levels
  • The first is simply a function of viral envelope protein, gp70
  • The envelope determines which cells the virus will enter
  • gp70 comes in three different flavors for gene therapy
  • Retroviruses obtained by cotransfection with pCL-Eco vector will infect mouse and rat cells, but not human cells
  • Species: Human, Mouse, Rat Specificity: Ectotropic (usually) (MoMuLV) Mouse and rat cells only (not human), Amphotropic (from 4070A MuLV) Most mammalian cells (but not hamster) 10A1 (MuLV) Most mammalian cells (including hamster) For large scale production, this plasmid may be amplified on LB plates containing 50μg/mL ampicillin Buffer: 10μg in 20μL 1x TE (10mM Tris, pH 7.5, 1mM EDTA)

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Show Difference

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Novus Biologicals™

NBP229540

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Storage Buffer:
10µg in 20µL 1x TE (10mM Tris, pH 7.5, 1mM EDTA)

Content And Storage:
Store at -20°C. Avoid freeze-thaw cycles.

Quantity:
10 μg

Vector:
pCL-Eco Retrovirus Packaging Vector

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Storage Buffer:
20µL 1x TE (10mM Tris, pH 7.5, 1mM EDTA)

Content And Storage:
Store at -20°C. Avoid freeze-thaw cycles.

Quantity:
2 μg

Vector:
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Storage Buffer:
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Content And Storage:
Storage is content dependent.

Quantity:
1 Kit

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Storage Buffer:
In electrophoresis sample buffer (62.5mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% Beta-mercaptoethanol).

Content And Storage:
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.

Quantity:
0.1 mg

Vector:
__