Promega Nano-Glo HiBiT Extracellular Detection System, 100ml

Description

• Simple Bioluminescent Detection of Cell-Surface Protein Expression
• The Nano-Glo HiBiT Extracellular Detection System quantitates cell surface or secreted protein expression in minutes using a simple add-mix-read assay format
• Using the nonlytic Nano-Glo™ HiBiT Extracellular Detection Reagent, any proteins that are tagged with the 11 amino acid HiBiT peptide and expressed outside of the cell can be specifically quantitated
• The detection reagent contains the complementary polypeptide LgBiT, which spontaneously interacts with the HiBiT tag to reconstitute the bright, luminescent NanoBiT™ enzyme
• Luminescence is proportional to the amount of HiBiT-tagged protein present outside of the cell over seven orders of magnitude
• Quantify HiBiT−Tagged Proteins Expressed on the Cell Surface Specific, live−cell detection of extracellular expressed or secreted proteins Simple add−mix−read assay format—no antibodies required Quantitate over 7 logs of linear dynamic range Each system contains sufficient components to prepare 100ml reagent Includes: Nano-Glo HiBiT Extracellular Buffer, N243B (100mL) Nano-Glo HiBiT Extracellular Substrate, N300B (2 × 1mL) LgBiT Protein, N401B (1mL)