Invitrogen™ Flp-In™-Jurkat Cell Line

Description

• These cells contain single stably integrated FRT site at transcriptionally active genomic locus Targeted integration of Flp-Inexpression vector ensures high-level expression of gene of interest Created by transfecting the parent cell lines with pFRT/ lac Zeo and selecting for stable Zeocin™-resistant clones Co-transfection of Flp-InCell Lines with Flp-Inexpression vector and Flp recombinase vector, pOG44, results in targeted integration of expression vector to same locus in every cell, ensuring homogeneous levels of gene expression Flp-In-Jurkat cell lines work well with vectors that express gene from CMV promoter Constitutive Expression, Mammalian Expression, Mammalian Expression Cell Lines, Protein Expression, Proteins, Expression, Isolation and Analysis, Targeted Integration