Invitrogen™ Vivid Colors™ pLenti6.3/V5-GW/EmGFP Expression Control Vector

Description

• The Vivid Colors™ pLenti6.3/V5-GW/EmGFP Expression Control Vector is a ViraPower™ HiPerform™ positive control lentiviral vector containing Emerald Green Fluorescent Protein (EmGFP)
• It is designed for use with the ViraPower™ HiPerform™ Lentiviral Expression Systems as a positive control to enable the detection of higher levels of EmGFP fluorescence following transfection in 293FT cells, as a titer control to produce an EmGFP-expressing lentivirus stock and as a transduction control following transduction in both dividing and non-dividing mammalian cells
• The vector has the CMV promoter for driving constitutive expression of EmGFP and the SV40 promoter for driving expression of the blasticidin stable selection marker
• The vector is equipped with two key genetic elements, making them Hiperform™ vectors: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in EmGFP expression compared to vectors lacking these elements
• This control vector is not designed for generating EmGFP fusion proteins and does not express the V5 epitope
• Advantages Lentivirus based expression of EmGFP in dividing and non-dividing mammalian cells Serve as a quick positive control for transfection and lentiviral production Serve as a quick titer control in determination of lentivirus titer Key Features Constitutive expression with CMV promoter High level expression of EmGFP without V5 epitope WPRE and cPPT sequences produce at least 4-fold increase in EmGFP expression compared to other Lenti vectors without these elements Blasticidin selection marker for stable selection Kit Includes pLenti6.3/V5-GW/EmGFP Expression Control Vector Related SKUs ViraPower™ HiPerform™ Lentiviral TOPO™ Expression Kit (Cat
• No
• K531000) ViraPower™ HiPerform™ Lentiviral Gateway™ Expression Kit (K533000) ViraPower™ HiPerform™ T-Rex™ Gateway™ Expression System (A11141) ViraPower™ HiPerform™ Promoterless Gateway™ Expression System (A11145)