QP1014
QuantiGene™ Plex Assay Kit
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | QP1014-Each-of-1 | In Stock | ₹ 2,21,260.00 |
QP1014 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 2,21,260.00
GST (18%): ₹ 39,826.80
Total Price: ₹ 2,61,086.80
Format
96-well
For Use With (Equipment)
Luminex instrument
Product Line
QuantiGene Plex
Quantity
3 Plates
Species
Universal
Detection Method
Fluorescence
For Use With (Application)
QuantiGene Plex Panel
Content And Storage
• 105 μL Proteinase K, –20°C• 810 μL Blocking Reagent, –20°C• 36 mL Label Probe Solution, 2–8°C• 36 mL Pre-Amplifier Solution, 2–8°C• 36 mL Amplifier Solution, 2–8°C• 108 μL SAPE, 2–8°C• 36 mL SAPE Diluent, 2–8°C• 36 mL Lysis Mixture, 15–30°C• 2.4 mL Wash Buffer Component 1, 15–30°C• 39 mL Wash Buffer Component 2, 15–30°C• 420 mL SAPE Wash Buffer, 15–30°C• 3 Magnetic Separation Plates, 15–30°C• 30 Plate Seals, 15–30°C• 3 Hybridization Plates, 15–30°C• 6 Pressure Seals, 15–30°C
Product Type
Multiplex Assay Kit
Shipping Condition
Blue Ice
Sample Type
Purified RNA, cell/blood/FFPE lysates, tissue homogenates
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Description
- Description The QuantiGene Plex Assay Kit contains the generic reagents, buffers, and plates necessary to perform the assay in 96-well plates, and must be used with a target-specific QuantiGene Plex panel (sold separately)
- The QuantiGene Plex Assay is a gene expression assay that enables multiplexed measurement of up to 80 RNA transcript targets
- The assay uses target-specific probes to capture the RNA of interest, and branched DNA technology then amplifies fluorescent signals which are read with a Luminex xMAP instrument
- • Difficult sample types —works with degraded and cross-linked RNA in FFPE tissues and blood • True multiplexing —measure up to 80 genes of interest and housekeeping genes in the same well with no cross-reactivity • Standardized platform —uses 96 or 384-well plate formats compatible with Luminex 200, MagPix, and FlexMap3D systems • Simple workflow —employs an ELISA-like workflow for direct hybridization of transcripts to beads and transcript labeling • Large inventory of validated genes —select from over 15,000 genes to create pathway and disease-themed panels • Rapid turnaround for custom panels —if we don’t have your gene(s), we can create a custom panel within two weeks Technology QuantiGene assays utilize branched DNA (bDNA) technology that has been used for decades in the VERSANT™ diagnostic assays for HIV-1, HCV, and HBV
- First, cells are lysed or tissue samples are homogenized to release the target RNA
- Second, the oligonucleotide probe set is incubated with the target RNA and Luminex xMAP bead set overnight
- During this incubation, the probes cooperatively hybridize to the target and capture probes bound to each bead, capturing the target RNA
- Third, signal amplification is performed via sequential hybridization of the bDNA preamplifier, amplifier, and label-probe molecules to the target
- Addition of streptavidin phycoerythrin generates a luminescent signal directly proportional to the amount of target mRNA present in the sample
- Applications QuantiGene Plex assay applications include compound screening of drug candidates, biomarker verification, siRNA knockdown efficiency, prospective and/or retrospective analysis of clinical samples, miRNA profiling, microarray validation, predictive toxicology, and detection of translocations and fusion genes.
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Show Difference
Format: 96-well
For Use With (Equipment): Luminex instrument
Product Line: QuantiGene Plex
Quantity: 3 Plates
Species: Universal
Detection Method: Fluorescence
For Use With (Application): QuantiGene Plex Panel
Content And Storage: • 105 μL Proteinase K, –20°C• 810 μL Blocking Reagent, –20°C• 36 mL Label Probe Solution, 2–8°C• 36 mL Pre-Amplifier Solution, 2–8°C• 36 mL Amplifier Solution, 2–8°C• 108 μL SAPE, 2–8°C• 36 mL SAPE Diluent, 2–8°C• 36 mL Lysis Mixture, 15–30°C• 2.4 mL Wash Buffer Component 1, 15–30°C• 39 mL Wash Buffer Component 2, 15–30°C• 420 mL SAPE Wash Buffer, 15–30°C• 3 Magnetic Separation Plates, 15–30°C• 30 Plate Seals, 15–30°C• 3 Hybridization Plates, 15–30°C• 6 Pressure Seals, 15–30°C
Product Type: Multiplex Assay Kit
Shipping Condition: Blue Ice
Sample Type: Purified RNA, cell/blood/FFPE lysates, tissue homogenates
Format:
96-well
For Use With (Equipment):
Luminex instrument
Product Line:
QuantiGene Plex
Quantity:
3 Plates
Species:
Universal
Detection Method:
Fluorescence
For Use With (Application):
QuantiGene Plex Panel
Content And Storage:
• 105 μL Proteinase K, –20°C• 810 μL Blocking Reagent, –20°C• 36 mL Label Probe Solution, 2–8°C• 36 mL Pre-Amplifier Solution, 2–8°C• 36 mL Amplifier Solution, 2–8°C• 108 μL SAPE, 2–8°C• 36 mL SAPE Diluent, 2–8°C• 36 mL Lysis Mixture, 15–30°C• 2.4 mL Wash Buffer Component 1, 15–30°C• 39 mL Wash Buffer Component 2, 15–30°C• 420 mL SAPE Wash Buffer, 15–30°C• 3 Magnetic Separation Plates, 15–30°C• 30 Plate Seals, 15–30°C• 3 Hybridization Plates, 15–30°C• 6 Pressure Seals, 15–30°C
Product Type:
Multiplex Assay Kit
Shipping Condition:
Blue Ice
Sample Type:
Purified RNA, cell/blood/FFPE lysates, tissue homogenates
Format: 96-well
For Use With (Equipment): Luminex instrument
Product Line: QuantiGene Plex
Quantity: 10 Plates
Species: Universal
Detection Method: Fluorescence
For Use With (Application): QuantiGene Plex Panel
Content And Storage: • 350 μL Proteinase K, –20°C• 2.8 mL Blocking Reagent, –20°C• 120 mL Label Probe Solution, 2–8°C• 120 mL Pre-Amplifier Solution, 2–8°C• 120 mL Amplifier Solution, 2–8°C• 360 μL SAPE, 2–8°C• 120 mL SAPE Diluent, 2–8°C• 120 mL Lysis Mixture, 15–30°C• 8 mL Wash Buffer Component 1, 15–30°C• 130 mL Wash Buffer Component 2, 15–30°C• 2 x 700 mL SAPE Wash Buffer, 15–30°C• 10 Magnetic Separation Plates, 15–30°C• 45 Plate Seals, 15–30°C• 10 Hybridization Plates, 15–30°C• 12 Pressure Seals, 15–30°C
Product Type: Multiplex Assay Kit
Shipping Condition: Blue Ice
Sample Type: Purified RNA, cell/blood/FFPE lysates, tissue homogenates
Format:
96-well
For Use With (Equipment):
Luminex instrument
Product Line:
QuantiGene Plex
Quantity:
10 Plates
Species:
Universal
Detection Method:
Fluorescence
For Use With (Application):
QuantiGene Plex Panel
Content And Storage:
• 350 μL Proteinase K, –20°C• 2.8 mL Blocking Reagent, –20°C• 120 mL Label Probe Solution, 2–8°C• 120 mL Pre-Amplifier Solution, 2–8°C• 120 mL Amplifier Solution, 2–8°C• 360 μL SAPE, 2–8°C• 120 mL SAPE Diluent, 2–8°C• 120 mL Lysis Mixture, 15–30°C• 8 mL Wash Buffer Component 1, 15–30°C• 130 mL Wash Buffer Component 2, 15–30°C• 2 x 700 mL SAPE Wash Buffer, 15–30°C• 10 Magnetic Separation Plates, 15–30°C• 45 Plate Seals, 15–30°C• 10 Hybridization Plates, 15–30°C• 12 Pressure Seals, 15–30°C
Product Type:
Multiplex Assay Kit
Shipping Condition:
Blue Ice
Sample Type:
Purified RNA, cell/blood/FFPE lysates, tissue homogenates
Format: 384-well
For Use With (Equipment): Invitrogen™ Luminex™ FLEXMAP 3D™ Instrument System
Product Line: QuantiGene Plex
Quantity: 2 Plates
Species: Universal
Detection Method: Fluorescence
For Use With (Application): QuantiGene Plex Panel
Content And Storage: • 200 μL Proteinase K, -20°C• 2.1 mL Blocking Reagent, 2°C to 8°C• 31 mL Label Probe Solution, 2°C to 8°C• 31 mL Pre-Amplifier Solution, 2°C to 8°C• 31 mL Amplifier Solution, 2°C to 8°C• 280 μL SAPE, 2°C to 8°C• 31 mL SAPE Diluent, 2°C to 8°C• 24 mL Lysis Mixture, room temperature• 6 mL Wash Buffer Component 1, room temperature• 100 mL Wash Buffer Component 2, room temperature• 1000 mL SAPE Wash Buffer, room temperature• 2 Hybridization plates (Day 1) 384-well, room temperature• 4 Manual Pressure seals (Day 1), room temperature• 4 Heat seals (Day 1), room temperature• 2 Magnetic Separation plates (Day 2), room temperature• 10 Plates seals (Day 2), room temperature
Product Type: QuantiGene Plex 384-well Assay Kit
Shipping Condition: Blue Ice
Sample Type: Purified RNA, cell/blood/FFPE lysates, tissue homogenates
Format:
384-well
For Use With (Equipment):
Invitrogen™ Luminex™ FLEXMAP 3D™ Instrument System
Product Line:
QuantiGene Plex
Quantity:
2 Plates
Species:
Universal
Detection Method:
Fluorescence
For Use With (Application):
QuantiGene Plex Panel
Content And Storage:
• 200 μL Proteinase K, -20°C• 2.1 mL Blocking Reagent, 2°C to 8°C• 31 mL Label Probe Solution, 2°C to 8°C• 31 mL Pre-Amplifier Solution, 2°C to 8°C• 31 mL Amplifier Solution, 2°C to 8°C• 280 μL SAPE, 2°C to 8°C• 31 mL SAPE Diluent, 2°C to 8°C• 24 mL Lysis Mixture, room temperature• 6 mL Wash Buffer Component 1, room temperature• 100 mL Wash Buffer Component 2, room temperature• 1000 mL SAPE Wash Buffer, room temperature• 2 Hybridization plates (Day 1) 384-well, room temperature• 4 Manual Pressure seals (Day 1), room temperature• 4 Heat seals (Day 1), room temperature• 2 Magnetic Separation plates (Day 2), room temperature• 10 Plates seals (Day 2), room temperature
Product Type:
QuantiGene Plex 384-well Assay Kit
Shipping Condition:
Blue Ice
Sample Type:
Purified RNA, cell/blood/FFPE lysates, tissue homogenates
Format: 96-well
For Use With (Equipment): __
Product Line: QuantiGene Plex
Quantity: 1 Plate
Species: __
Detection Method: __
For Use With (Application): QuantiGene Plex Assay
Content And Storage: • 0.8 mL Wash Buffer Component 1• 13 mL Wash Buffer Component 2Store at room temperature.
Product Type: Wash Buffer
Shipping Condition: Room Temperature
Sample Type: __
Format:
96-well
For Use With (Equipment):
__
Product Line:
QuantiGene Plex
Quantity:
1 Plate
Species:
__
Detection Method:
__
For Use With (Application):
QuantiGene Plex Assay
Content And Storage:
• 0.8 mL Wash Buffer Component 1• 13 mL Wash Buffer Component 2Store at room temperature.
Product Type:
Wash Buffer
Shipping Condition:
Room Temperature
Sample Type:
__