QP1016
QuantiGene™ Plex 384-well Assay Kit
Manufacturer: Invitrogen
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | QP1016-Each-of-1 | In Stock | ₹ 4,83,000.00 |
QP1016 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 4,83,000.00
GST (18%): ₹ 86,940.00
Total Price: ₹ 5,69,940.00
Format
384-well
For Use With (Equipment)
Invitrogen™ Luminex™ FLEXMAP 3D™ Instrument System
Product Line
QuantiGene Plex
Quantity
2 Plates
Species
Universal
Detection Method
Fluorescence
For Use With (Application)
QuantiGene Plex Panel
Content And Storage
• 200 μL Proteinase K, -20°C• 2.1 mL Blocking Reagent, 2°C to 8°C• 31 mL Label Probe Solution, 2°C to 8°C• 31 mL Pre-Amplifier Solution, 2°C to 8°C• 31 mL Amplifier Solution, 2°C to 8°C• 280 μL SAPE, 2°C to 8°C• 31 mL SAPE Diluent, 2°C to 8°C• 24 mL Lysis Mixture, room temperature• 6 mL Wash Buffer Component 1, room temperature• 100 mL Wash Buffer Component 2, room temperature• 1000 mL SAPE Wash Buffer, room temperature• 2 Hybridization plates (Day 1) 384-well, room temperature• 4 Manual Pressure seals (Day 1), room temperature• 4 Heat seals (Day 1), room temperature• 2 Magnetic Separation plates (Day 2), room temperature• 10 Plates seals (Day 2), room temperature
Product Type
QuantiGene Plex 384-well Assay Kit
Shipping Condition
Blue Ice
Sample Type
Purified RNA, cell/blood/FFPE lysates, tissue homogenates
Related Products
Description
- Description The QuantiGene Plex 384-well Assay Kit contains the generic reagents, buffers, and plates necessary to perform the assay in 384-well plates and must be used with a target-specific QuantiGene Plex panel (sold separately)
- The QuantiGene Plex Assay is a gene expression assay that enables multiplexed measurement of up to 80 RNA transcript targets
- The assay uses target-specific probes to capture the RNA of interest, and branched DNA technology then amplifies fluorescent signals, which are read with a Luminex xMAP instrument (FlexMap3D)
- Features of the QuantiGene Plex 384-well Assay Kit include: • Accommodates difficult sample types —works with degraded and cross-linked RNA in FFPE tissues and blood • True multiplexing —measures up to 80 genes of interest and housekeeping genes in the same well with no cross-reactivity • Standardized platform —uses 384-well plate format compatible with Luminex FlexMap3D systems • Simple workflow —employs an ELISA-like workflow for direct hybridization of transcripts to beads and transcript labeling • Large inventory of validated genes —select from over 22,000 genes to create pathway and disease-themed panels • Rapid turnaround for custom panels —a custom panel can be created within three weeks Technology QuantiGene assays utilize branched DNA (bDNA) technology that has been used for decades in various applications and areas of biology
- First, cells are lysed or tissue samples are homogenized to release the target RNA
- Second, the oligonucleotide probe set is incubated with the target RNA and Luminex xMAP bead set overnight
- During this incubation, the probes cooperatively hybridize to the target and capture probes bound to each bead, capturing the target RNA
- Third, signal amplification is performed via sequential hybridization of the bDNA pre-amplifier, amplifier, and label probe molecules to the target
- Addition of streptavidin phycoerythrin generates a luminescent signal directly proportional to the amount of target mRNA present in the sample
- Applications QuantiGene Plex 384-well Assay applications include compound screening of drug candidates (primary screening), biomarker verification (secondary screening), siRNA knockdown efficiency, prospective and/or retrospective analysis of samples, microarray validation, predictive toxicology, and detection of translocations and fusion genes.
Compare Similar Items
Show Difference
Format: 384-well
For Use With (Equipment): Invitrogen™ Luminex™ FLEXMAP 3D™ Instrument System
Product Line: QuantiGene Plex
Quantity: 2 Plates
Species: Universal
Detection Method: Fluorescence
For Use With (Application): QuantiGene Plex Panel
Content And Storage: • 200 μL Proteinase K, -20°C• 2.1 mL Blocking Reagent, 2°C to 8°C• 31 mL Label Probe Solution, 2°C to 8°C• 31 mL Pre-Amplifier Solution, 2°C to 8°C• 31 mL Amplifier Solution, 2°C to 8°C• 280 μL SAPE, 2°C to 8°C• 31 mL SAPE Diluent, 2°C to 8°C• 24 mL Lysis Mixture, room temperature• 6 mL Wash Buffer Component 1, room temperature• 100 mL Wash Buffer Component 2, room temperature• 1000 mL SAPE Wash Buffer, room temperature• 2 Hybridization plates (Day 1) 384-well, room temperature• 4 Manual Pressure seals (Day 1), room temperature• 4 Heat seals (Day 1), room temperature• 2 Magnetic Separation plates (Day 2), room temperature• 10 Plates seals (Day 2), room temperature
Product Type: QuantiGene Plex 384-well Assay Kit
Shipping Condition: Blue Ice
Sample Type: Purified RNA, cell/blood/FFPE lysates, tissue homogenates
Format:
384-well
For Use With (Equipment):
Invitrogen™ Luminex™ FLEXMAP 3D™ Instrument System
Product Line:
QuantiGene Plex
Quantity:
2 Plates
Species:
Universal
Detection Method:
Fluorescence
For Use With (Application):
QuantiGene Plex Panel
Content And Storage:
• 200 μL Proteinase K, -20°C• 2.1 mL Blocking Reagent, 2°C to 8°C• 31 mL Label Probe Solution, 2°C to 8°C• 31 mL Pre-Amplifier Solution, 2°C to 8°C• 31 mL Amplifier Solution, 2°C to 8°C• 280 μL SAPE, 2°C to 8°C• 31 mL SAPE Diluent, 2°C to 8°C• 24 mL Lysis Mixture, room temperature• 6 mL Wash Buffer Component 1, room temperature• 100 mL Wash Buffer Component 2, room temperature• 1000 mL SAPE Wash Buffer, room temperature• 2 Hybridization plates (Day 1) 384-well, room temperature• 4 Manual Pressure seals (Day 1), room temperature• 4 Heat seals (Day 1), room temperature• 2 Magnetic Separation plates (Day 2), room temperature• 10 Plates seals (Day 2), room temperature
Product Type:
QuantiGene Plex 384-well Assay Kit
Shipping Condition:
Blue Ice
Sample Type:
Purified RNA, cell/blood/FFPE lysates, tissue homogenates
Format: 96-well
For Use With (Equipment): __
Product Line: QuantiGene Plex
Quantity: 1 Plate
Species: __
Detection Method: __
For Use With (Application): QuantiGene Plex Assay
Content And Storage: • 0.8 mL Wash Buffer Component 1• 13 mL Wash Buffer Component 2Store at room temperature.
Product Type: Wash Buffer
Shipping Condition: Room Temperature
Sample Type: __
Format:
96-well
For Use With (Equipment):
__
Product Line:
QuantiGene Plex
Quantity:
1 Plate
Species:
__
Detection Method:
__
For Use With (Application):
QuantiGene Plex Assay
Content And Storage:
• 0.8 mL Wash Buffer Component 1• 13 mL Wash Buffer Component 2Store at room temperature.
Product Type:
Wash Buffer
Shipping Condition:
Room Temperature
Sample Type:
__
Format: 96-well
For Use With (Equipment): __
Product Line: QuantiGene Plex
Quantity: 3 Plates
Species: __
Detection Method: __
For Use With (Application): QuantiGene Plex Assay
Content And Storage: • 2.4 mL Wash Buffer Component 1• 39 mL Wash Buffer Component 2Store at room temperature.
Product Type: Wash Buffer
Shipping Condition: Room Temperature
Sample Type: __
Format:
96-well
For Use With (Equipment):
__
Product Line:
QuantiGene Plex
Quantity:
3 Plates
Species:
__
Detection Method:
__
For Use With (Application):
QuantiGene Plex Assay
Content And Storage:
• 2.4 mL Wash Buffer Component 1• 39 mL Wash Buffer Component 2Store at room temperature.
Product Type:
Wash Buffer
Shipping Condition:
Room Temperature
Sample Type:
__
Format: 96-well
For Use With (Equipment): __
Product Line: QuantiGene Plex
Quantity: 10 Plates
Species: __
Detection Method: __
For Use With (Application): QuantiGene Plex Assay
Content And Storage: • 8 mL Wash Buffer Component 1• 130 mL Wash Buffer Component 2Store at room temperature.
Product Type: Wash Buffer
Shipping Condition: Room Temperature
Sample Type: __
Format:
96-well
For Use With (Equipment):
__
Product Line:
QuantiGene Plex
Quantity:
10 Plates
Species:
__
Detection Method:
__
For Use With (Application):
QuantiGene Plex Assay
Content And Storage:
• 8 mL Wash Buffer Component 1• 130 mL Wash Buffer Component 2Store at room temperature.
Product Type:
Wash Buffer
Shipping Condition:
Room Temperature
Sample Type:
__