A39391
Thermo Scientific™ EquiPhi29™ DNA Polymerase
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | A39391-Each-of-1 | In Stock | ₹ 32,574.00 |
A39391 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 32,574.00
GST (18%): ₹ 5,863.32
Total Price: ₹ 38,437.32
Product Type
Stand-alone enzyme
Polymerase
EquiPhi29 DNA Polymerase
Content And Storage
• EquiPhi29 DNA Polymerase (100 μL at 10 U/μL)• 10X EquiPhi29 DNA Polymerase Reaction Buffer (1.0 mL)• 110 mM DTT (0.25 mL)Store at –15 to –25°C.
Shipping Condition
Dry Ice
Reaction Time
2 hours
For Use With (Application)
MDA-WGA, RCA
Quantity
1000 U
Optimal Reaction Temperature
42°C
Concentration
10 U/μL
Related Products
Description
- Thermo Scientific EquiPhi29 DNA Polymerase is a proprietary phi29 DNA polymerase mutant developed through in vitro protein evolution
- This enzyme is significantly improved over phi29 DNA polymerase in protein thermostability, reaction speed, product yield, and amplification bias, while retaining all the benefits of the wild-type enzyme
- EquiPhi29 DNA Polymerase possesses strong strand displacement activity and 3'→5' proofreading exonuclease activity that acts preferentially on single-stranded DNA or RNA
- Features of EquiPhi29 DNA Polymerase • Highest processivity and strand displacement activity—more than 70 kb long DNA stretches can be synthesized • Low amplification bias • Extremely high yields of amplified DNA, even from small amounts of template • Highly accurate DNA synthesis in a short time Applications EquiPhi29 DNA Polymerase may be used in a wide variety of applications including: • Unbiased whole genome amplification (WGA) • Rolling circle amplification (RCA) • Protein-primed DNA amplification • Cell-free cloning of lethal DNA • In situ genotyping with padlock probes • RNA-primed DNA amplification Notes 1
- The addition of pyrophosphatase to the reaction mixture with EquiPhi29 DNA Polymerase may further enhance DNA synthesis 2
- Because of the enzyme's 3'→5' proofreading exonuclease, 3’-modified primers are highly recommended
- 3
- EquiPhi29 DNA Polymerase can amplify DNA in a wide range of temperatures (30–45°C), with an optimal reaction temperature of 42°C.
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Show Difference
Product Type: Stand-alone enzyme
Polymerase: EquiPhi29 DNA Polymerase
Content And Storage: • EquiPhi29 DNA Polymerase (100 μL at 10 U/μL)• 10X EquiPhi29 DNA Polymerase Reaction Buffer (1.0 mL)• 110 mM DTT (0.25 mL)Store at –15 to –25°C.
Shipping Condition: Dry Ice
Reaction Time: 2 hours
For Use With (Application): MDA-WGA, RCA
Quantity: 1000 U
Optimal Reaction Temperature: 42°C
Concentration: 10 U/μL
Product Type:
Stand-alone enzyme
Polymerase:
EquiPhi29 DNA Polymerase
Content And Storage:
• EquiPhi29 DNA Polymerase (100 μL at 10 U/μL)• 10X EquiPhi29 DNA Polymerase Reaction Buffer (1.0 mL)• 110 mM DTT (0.25 mL)Store at –15 to –25°C.
Shipping Condition:
Dry Ice
Reaction Time:
2 hours
For Use With (Application):
MDA-WGA, RCA
Quantity:
1000 U
Optimal Reaction Temperature:
42°C
Concentration:
10 U/μL
Product Type: Stand-alone enzyme
Polymerase: EquiPhi29 DNA Polymerase
Content And Storage: • 5 x 100 μL EquiPhi29 DNA Polymerase (10 U/μL)• 3 x 1 mL EquiPhi29 DNA Polymerase Reaction Buffer (10X)• 0.25 mL DTT (100 mM)Store at –15 to –25°C.
Shipping Condition: Dry Ice
Reaction Time: 2 hours
For Use With (Application): MDA-WGA, RCA
Quantity: 5000 U
Optimal Reaction Temperature: 42°C
Concentration: 10 U/μL
Product Type:
Stand-alone enzyme
Polymerase:
EquiPhi29 DNA Polymerase
Content And Storage:
• 5 x 100 μL EquiPhi29 DNA Polymerase (10 U/μL)• 3 x 1 mL EquiPhi29 DNA Polymerase Reaction Buffer (10X)• 0.25 mL DTT (100 mM)Store at –15 to –25°C.
Shipping Condition:
Dry Ice
Reaction Time:
2 hours
For Use With (Application):
MDA-WGA, RCA
Quantity:
5000 U
Optimal Reaction Temperature:
42°C
Concentration:
10 U/μL
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