Thermo Scientific™ EquiPhi29™ DNA Polymerase

Description

• Thermo Scientific EquiPhi29 DNA Polymerase is a proprietary phi29 DNA polymerase mutant developed through in vitro protein evolution
• This enzyme is significantly improved over phi29 DNA polymerase in protein thermostability, reaction speed, product yield, and amplification bias, while retaining all the benefits of the wild-type enzyme
• EquiPhi29 DNA Polymerase possesses strong strand displacement activity and 3'→5' proofreading exonuclease activity that acts preferentially on single-stranded DNA or RNA
• Features of EquiPhi29 DNA Polymerase • Highest processivity and strand displacement activity—more than 70 kb long DNA stretches can be synthesized • Low amplification bias • Extremely high yields of amplified DNA, even from small amounts of template • Highly accurate DNA synthesis in a short time Applications EquiPhi29 DNA Polymerase may be used in a wide variety of applications including: • Unbiased whole genome amplification (WGA) • Rolling circle amplification (RCA) • Protein-primed DNA amplification • Cell-free cloning of lethal DNA • In situ genotyping with padlock probes • RNA-primed DNA amplification Notes 1
• The addition of pyrophosphatase to the reaction mixture with EquiPhi29 DNA Polymerase may further enhance DNA synthesis 2
• Because of the enzyme's 3'→5' proofreading exonuclease, 3’-modified primers are highly recommended
• 3
• EquiPhi29 DNA Polymerase can amplify DNA in a wide range of temperatures (30–45°C), with an optimal reaction temperature of 42°C.