Thermo Scientific™ phi29 DNA Polymerase (10 U/μL)

Description

• Thermo Scientific phi29 DNA Polymerase is a highly processive polymerase featuring strong strand displacement activity, which allows for highly efficient isothermal DNA amplification
• Produced from E
• coli cells with a cloned gene 2 of Bacillus subtilis phage phi29, phi29 DNA Polymerase possesses 3'→5' proofreading exonuclease activity that acts preferentially on single-stranded DNA or RNA
• Thermo Scientific phi29 DNA Polymerase is a highly processive polymerase featuring strong strand displacement activity, which allows for highly efficient isothermal DNA amplification
• Produced from E
• coli cells with a cloned gene 2 of Bacillus subtilis phage phi29, phi29 DNA Polymerase possesses 3'→5' proofreading exonuclease activity that acts preferentially on single-stranded DNA or RNA
• Features of phi29 DNA Polymerase • Excellent processivity and strand displacement activity—more than 70 kb long DNA stretches can be synthesized • Highly accurate DNA synthesis • High yields of amplified DNA even from small amounts of template Applications Phi29 DNA Polymerase may be used in a wide variety of applications including: • Rolling circle amplification (RCA)—generation of periodic DNA nanotemplates • Multiple displacement amplification (MDA) • Unbiased amplification of whole genome (WGA) (see figure below) • Amplification of DNA for SNP and STR detection • Cell-free amplification of DNA from single cells, pathogenic organisms, or metagenomes • DNA template preparation for sequencing • Protein-primed DNA amplification • Recombination based-cloning • Cell-free cloning of lethal DNA • RNA-primed DNA amplification Notes 1
• The addition of pyrophosphatase to the reaction mixture with phi29 DNA Polymerase may enhance DNA synthesis
• 2
• Because of the enzymes’s 3’→5’ proofreading exonuclease, 3'-modified primers are highly recommended.