A56656
Invitrogen™ Lyo-ready Bst DNA Polymerase
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | A56656-Each-of-1 | In Stock | ₹ 24,475.00 |
A56656 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 24,475.00
GST (18%): ₹ 4,405.50
Total Price: ₹ 28,880.50
Product Type
Stand-alone enzyme
Polymerase
Lyo-ready Bst DNA Polymerase
Content And Storage
• Lyo-ready Bst DNA Polymerase (5 x 200 μL at 6 U/μL)• 10X Bst Reaction Buffer (3 x 1 mL)• 200 mM MgCl2(1 mL)• 5 M Betaine (3 x 1 mL)Store at –15 to –25°C.
Optimal Reaction Temperature
65°C
Concentration
6 U/μL
For Use With (Application)
LAMP, RT-LAMP
Quantity
6000 U
No. of Reactions
1000 Reactions
Shipping Condition
Dry Ice
Reaction Time
≤10 minutes
Related Products
Description
- Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors
- By retaining strong strand-displacement activity, this enzyme helps enable highly efficient isothermal amplifications such as loop-mediated isothermal amplification (LAMP)
- Invitrogen Lyo-ready Bst DNA Polymerase is a proprietary mutant of Bst DNA polymerase, large fragment, with a fast reaction speed, increased sensitivity, and increased tolerance to inhibitors
- By retaining strong strand-displacement activity, this enzyme helps enable highly efficient isothermal amplifications such as loop-mediated isothermal amplification (LAMP)
- Lyo-ready Bst DNA Polymerase possesses 5´→3´ DNA polymerase activity and lacks 5´→3´ exonuclease activity and 3´→5´ exonuclease activity
- It is also a glycerol-free enzyme, enabling assay lyophilization and use in microfluidics-based technologies
- Lyo-ready Bst DNA Polymerase features • Fast target detection in as little as 10 minutes • Enhanced sensitivity down to 50 copies using optimized reaction buffer in (RT-)LAMP reaction • Robust amplification, even from inhibitor-containing RNA or DNA samples • Ability to optimize LAMP or RT-LAMP reactions to fit your needs Applications Lyo-ready Bst DNA Polymerase can be used for a wide variety of applications including: • Loop-mediated isothermal amplification (LAMP) • Whole genome amplification (WGA) • Rolling circle amplification (RCA) • Recombinase polymerase amplification (RPA) • Helicase-dependent amplification (HDA) • Exponential amplification reaction (EXPAR) Notes 1
- If performing real-time LAMP detection with SYTO 9 Green Fluorescent Nucleic Acid Stain, dilute the stock to 50 μM with nuclease-free water before every use
- 2
- SuperScript IV Reverse Transcriptase and RNaseOUT Recombinant Ribonuclease Inhibitor are recommended for reactions containing RNA targets.
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Product Type: Stand-alone enzyme
Polymerase: Lyo-ready Bst DNA Polymerase
Content And Storage: • Lyo-ready Bst DNA Polymerase (5 x 200 μL at 6 U/μL)• 10X Bst Reaction Buffer (3 x 1 mL)• 200 mM MgCl2(1 mL)• 5 M Betaine (3 x 1 mL)Store at –15 to –25°C.
Optimal Reaction Temperature: 65°C
Concentration: 6 U/μL
For Use With (Application): LAMP, RT-LAMP
Quantity: 6000 U
No. of Reactions: 1000 Reactions
Shipping Condition: Dry Ice
Reaction Time: ≤10 minutes
Product Type:
Stand-alone enzyme
Polymerase:
Lyo-ready Bst DNA Polymerase
Content And Storage:
• Lyo-ready Bst DNA Polymerase (5 x 200 μL at 6 U/μL)• 10X Bst Reaction Buffer (3 x 1 mL)• 200 mM MgCl2(1 mL)• 5 M Betaine (3 x 1 mL)Store at –15 to –25°C.
Optimal Reaction Temperature:
65°C
Concentration:
6 U/μL
For Use With (Application):
LAMP, RT-LAMP
Quantity:
6000 U
No. of Reactions:
1000 Reactions
Shipping Condition:
Dry Ice
Reaction Time:
≤10 minutes
Product Type: Stand-alone enzyme
Polymerase: Lyo-ready Bst DNA Polymerase
Content And Storage: • Lyo-ready Bst DNA Polymerase (30 μL at 40 U/μL)• 10X Bst Reaction Buffer (1 mL)• 200 mM MgCl2(1 mL)• 5 M Betaine (1 mL)Store at –15 to –25°C.
Optimal Reaction Temperature: 65°C
Concentration: 40 U/μL
For Use With (Application): LAMP, RT-LAMP
Quantity: 1200 U
No. of Reactions: 200 Reactions
Shipping Condition: Dry Ice
Reaction Time: ≤10 minutes
Product Type:
Stand-alone enzyme
Polymerase:
Lyo-ready Bst DNA Polymerase
Content And Storage:
• Lyo-ready Bst DNA Polymerase (30 μL at 40 U/μL)• 10X Bst Reaction Buffer (1 mL)• 200 mM MgCl2(1 mL)• 5 M Betaine (1 mL)Store at –15 to –25°C.
Optimal Reaction Temperature:
65°C
Concentration:
40 U/μL
For Use With (Application):
LAMP, RT-LAMP
Quantity:
1200 U
No. of Reactions:
200 Reactions
Shipping Condition:
Dry Ice
Reaction Time:
≤10 minutes
Product Type: Fetal Bovine Serum
Polymerase: __
Content And Storage: Store at ≤-10°C.
Optimal Reaction Temperature: __
Concentration: __
For Use With (Application): __
Quantity: 50 mL
No. of Reactions: __
Shipping Condition: Dry Ice
Reaction Time: __
Product Type:
Fetal Bovine Serum
Polymerase:
__
Content And Storage:
Store at ≤-10°C.
Optimal Reaction Temperature:
__
Concentration:
__
For Use With (Application):
__
Quantity:
50 mL
No. of Reactions:
__
Shipping Condition:
Dry Ice
Reaction Time:
__
Product Type: Fetal Bovine Serum
Polymerase: __
Content And Storage: Store at ≤-10°C.
Optimal Reaction Temperature: __
Concentration: __
For Use With (Application): __
Quantity: 10 x 50 mL
No. of Reactions: __
Shipping Condition: Dry Ice
Reaction Time: __
Product Type:
Fetal Bovine Serum
Polymerase:
__
Content And Storage:
Store at ≤-10°C.
Optimal Reaction Temperature:
__
Concentration:
__
For Use With (Application):
__
Quantity:
10 x 50 mL
No. of Reactions:
__
Shipping Condition:
Dry Ice
Reaction Time:
__