Thermo Scientific™ EquiPhi29™ DNA Amplification Kit

Description

• The Thermo Scientific EquiPhi29 DNA Amplification Kit includes all required components for fast, sensitive, and efficient isothermal DNA amplification, which includes rolling circle amplification (RCA) and multiple displacement amplification – whole genome amplification (MDA-WGA)
• Features of the EquiPhi29 DNA Amplification Kit include: • Fast DNA amplification in less than two hours • Sensitivity from 1 fg of input DNA • Maximal yield of 17 µg amplified DNA • Compatibility with different types of sample input material (e.g., purified DNA, liquid media culture, agar plate colonies) • Compatibility with various downstream applications such as DNA sequencing (Sanger sequencing, next-generation sequencing), digestion with restriction enzymes, cell-free DNA enrichment, and cell-free protein expression • All required components for DNA amplification in RCA or MDA-WGA reactions The kit contains EquPhi29 DNA Polymerase, a proprietary phi29 DNA polymerase mutant that possesses strong strand displacement activity and enables high thermostability, reaction speed, and product yield and low amplification bias
• Also included is an optimized reaction buffer, exo-resistant random primer, pyrophosphatase, and dNTPs
• The addition of pyrophosphatase to the reaction mixture enhances DNA amplification and facilitates sample handling.