Thermo Scientific™ RNA Gel Loading Dye (2X)

Description

• 2X RNA Loading Dye is recommended for preparation of RiboRuler RNA ladders and RNA samples for electrophoresis on agarose or polyacrylamide gels
• It contains the tracking dyes bromophenol blue and xylene cyanol FF as well as the intercalating dye ethidium bromide
• In most denaturing agarose gel systems, bromophenol blue migrates slightly faster than human 5S rRNA, whereas xylene cyanol FF migrates slightly slower than 18S rRNA
• 2X RNA Loading Dye contains the denaturing agent formamide, which allows RNA fragments to separate according to size even during non-denaturing electrophoresis
• Formamide also stabilizes RNA
• Highlights Two-color tracking of DNA, RNA migration during electrophoresis No DNA, RNA masking during gel exposure to UV light Denaturation of DNA, RNA due to the presence of formamide