Invitrogen™ RecoverAll™ Multi-Sample RNA/DNA Isolation Workflow

Description

• The RecoverAll™ Multi-Sample RNA/DNA Workflow is a complete workflow package for sequential extraction of RNA and DNA from the same formaldehyde- or paraformaldehyde-fixed paraffin-embedded (FFPE) tissue sample
• The recovered RNA and DNA are ready for use in downstream applications such as quantitative real-time RT-PCR and targeted sequencing using the Oncomine™ Cancer Research Panel
• This workflow package includes RecoverAll™ Total Nucleic Acid Isolation kits, elution tubes, and nuclease-free water
• Features include: • Recovers RNA and DNA in separate eluates from the same FFPE section • Relies on proven Ambion™ RecoverAll technology • Processes four 20-μm sections per reaction Special workflow provides more information from less sample input FFPE samples are precious, which often requires the choosing of one extraction method over another to save sample
• With the RecoverAll Multi-Sample RNA/DNA Workflow, recovering RNA and DNA from the same FFPE tissue sample in a single workflow eliminates the need to choose which nucleic acid to recover from a particular FFPE section
• First, FFPE slide-mounted or tissue blocks sections are deparaffinized and digested with protease
• The samples are then loaded on a filter cartridge; DNA remains bound to the filter cartridge while RNA flows through
• To recover RNA, the flow-through is applied to a new filter cartridge, treated with DNase, then eluted from the filter
• To recover DNA, the DNA bound to the filter cartridge is treated with RNase A, then eluted from the filter.