Applied Biosystems™ MagMAX™ Viral RNA Isolation Kit

Description

• RNA isolation from cell-free samples presents some unique challenges compared to RNA isolation from tissues or cultured cells
• These include low viral titers, large volumes to be processed, and sample complexity
• Also, the procedure must be compatible with real-time qRT-PCR
• The MagMAX™ Viral RNA isolation kits address all of these issues, without the need for organic solvents or RNA precipitation, eliminating the problems often encountered with filter-based RNA isolation methods such as clogging, large elution volumes, and inconsistent yields
• More effective recovery of viral RNA and DNA from diverse sample types Improved qRT-PCR results Ideal when working with low viral concentrations Improved yields and reproducibility MagMAX™ Viral RNA Isolation Kit The improved version of the MagMAX™ Viral RNA Isolation Kit provides better viral particle lysis and more effective recovery of both viral RNA and DNA from a variety of cell-free biological samples, including plasma, sera, and swabs
• The results of these changes are improved yields and reproducibility
• Enhanced genomic DNA removal results in better qRT-PCR results
• The MagMAX™ Viral RNA Isolation Kit can efficiently isolate viral RNA and DNA from samples as large as 400 μL
• RNA and DNA recovery is typically greater than 75%, but may vary depending on sample type
• The RNA recovered with the kit is of high quality and purity and is suitable for real-time RT-PCR
• Accessory Products Lysis/Binding Solution Concentrate, Wash Solution 1 Concentrate, Wash Solution 2 Concentrate, and Carrier RNA are components of the MagMAX™ Viral RNA Isolation Kits that may also be purchased separately
• Additional Processing Plates and Lids: we recommend 96-well plates and lids from Phenix Research Products (#MPU-8117, #MPE-8019)
• Benefits of Magnetic Bead-based Isolation Magnetic beads offer many benefits for isolating RNA from cells and viral RNA from cell-free samples
• Ambion’s MagMAX™technology binds RNA more efficiently than glass fiber filter methods resulting in higher RNA yields
• The RNA yields are more consistent, both from experiment to experiment, and over a broad range of sample sizes
• MagMAX™magnetic bead technology eliminates filter clogging due to cellular particulates in samples
• Since only a small volume of magnetic beads is needed for high efficiency binding, the bound RNA can be eluted in just 20 to 50 μL of nuclease free water
• This makes the MagMAX™ magnetic bead technology extremely useful for concentrating RNA from large, dilute samples.