AM1836
Applied Biosystems™ MagMAX™-96 Viral RNA Isolation Kit
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | AM1836-Each-of-1 | In Stock | ₹ 47,229.12 |
AM1836 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 47,229.12
GST (18%): ₹ 8,501.242
Total Price: ₹ 55,730.362
Content And Storage
Carrier RNA and Lysis Binding Enhancer is stored at –20°C; RNA Binding Beads are stored at 4°C. Wash Solution 2 Concentrate and Elution Buffer may be stored at either 4°C or room temperature. Lysis⁄Binding Solution Concentrate, Wash Solution 1 Concentrate, and Processing Plates and Lids are stored at room temperature.
Isolation Technology
Magnetic Bead
Sample Type
Cell-free Biological Fluids, Liquid Samples (e.g. Serum), Viral Samples
High-throughput Compatibility
High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols
For Use With (Application)
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Product Line
MagMAX™
Starting Material Amount
Up to 50μl of sample
Yield
5 ng
Format
96-well Plate
Purification Time
45 min.
Final Product Type
Viral DNA⁄RNA
For Use With (Equipment)
MagMAX™ Express-96 Magnetic Particle Processor, KingFisher 96, MagMAX™ Express-96 Standard Magnetic Particle Processor
No. of Reactions
96
Scale
Midi
Target
Viral DNA/RNA
Related Products
Description
- Perfect for working with low viral concentrations, the kit contains sufficient reagents for 5 × 96 reactions
- Benefits of MagMAX™ Magnetic Bead-Based Purification: Magnetic beads offer many benefits compared to other technologies for isolating RNA
- Beads bind RNA more efficiently than glass fiber filters, resulting in higher and more consistent RNA yields
- Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples
- Since only a small volume of magnetic beads is needed for high-efficiency binding, the bound RNA can be eluted in just 20–50μL of nuclease-free water, concentrating RNA from large, dilute samples
- Optimized for Isolation of Virus from Biofluids: The Applied Biosystems™ MagMAX™ Viral RNA Isolation Kits have been optimized for use with biological fluids and cell-free samples such as serum, plasma, swabs, and cell culture media
- These samples often present unique challenges when purifying RNA, including low viral titers, large volumes to be processed, and sample complexity
- Without the need for organic solvents or RNA precipitation, the MagMAX™ Viral RNA Isolation Kit eliminates the problems often encountered with other RNA isolation methods such filter clogging, large elution volumes, and inconsistent yields
- With this kit, DNA and RNA input levels ranging from 20 copies to 25 x 10 6 copies have been quantitatively recovered from plasma, serum, and milk
- And even with less sample input, pathogen detection was as sensitive (equivalent C Ts ) or more sensitive (lower C Ts ) using nucleic acid isolated with the MagMAX™ kit when compared to nucleic acid isolated using another company's product
- Downloadable protocols for the use of this kit with specific liquid handling systems are available at our Automation Resource
- Accessory Products: Carrier RNA (SKU#4382878), Lysis/Binding Solution Concentrate (SKU#AM8500), Wash Solution 1 Concentrate (SKU#AM8504), and Wash Solution 2 Concentrate (SKU#AM8640) are components of the MagMAX™ Viral RNA Isolation Kits that may also be purchased separately
- A 96-well magnetic ring stand is required to use this kit
- The Magnetic Stand-96 (SKU#AM10027) and the 96-well Magnetic Ring Stand (SKU#AM10050) are designed for paramagnetic bead precipitation from standard 96- well, U-bottom microplates
- The Magnetic-Ring Stand pellets the magnetic beads in a circle shape, enabling easy removal of supernatant by a robot or multichannel pipettors
- For additional processing plates, Matrix 96-Well U Bottom Polypropylene Plates (Thermo Scientific, Item# 4917) are recommended
- For an NVSL-validated version of this kit, please see the MagMAX™-96 AI/ND Viral RNA Isolation Kits (SKU#1929 and SKU#AM1835).
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Content And Storage: Carrier RNA and Lysis Binding Enhancer is stored at –20°C; RNA Binding Beads are stored at 4°C. Wash Solution 2 Concentrate and Elution Buffer may be stored at either 4°C or room temperature. Lysis⁄Binding Solution Concentrate, Wash Solution 1 Concentrate, and Processing Plates and Lids are stored at room temperature.
Isolation Technology: Magnetic Bead
Sample Type: Cell-free Biological Fluids, Liquid Samples (e.g. Serum), Viral Samples
High-throughput Compatibility: High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols
For Use With (Application): Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Product Line: MagMAX™
Starting Material Amount: Up to 50μl of sample
Yield: 5 ng
Format: 96-well Plate
Purification Time: 45 min.
Final Product Type: Viral DNA⁄RNA
For Use With (Equipment): MagMAX™ Express-96 Magnetic Particle Processor, KingFisher 96, MagMAX™ Express-96 Standard Magnetic Particle Processor
No. of Reactions: 96
Scale: Midi
Target: Viral DNA/RNA
Content And Storage:
Carrier RNA and Lysis Binding Enhancer is stored at –20°C; RNA Binding Beads are stored at 4°C. Wash Solution 2 Concentrate and Elution Buffer may be stored at either 4°C or room temperature. Lysis⁄Binding Solution Concentrate, Wash Solution 1 Concentrate, and Processing Plates and Lids are stored at room temperature.
Isolation Technology:
Magnetic Bead
Sample Type:
Cell-free Biological Fluids, Liquid Samples (e.g. Serum), Viral Samples
High-throughput Compatibility:
High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols
For Use With (Application):
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Product Line:
MagMAX™
Starting Material Amount:
Up to 50μl of sample
Yield:
5 ng
Format:
96-well Plate
Purification Time:
45 min.
Final Product Type:
Viral DNA⁄RNA
For Use With (Equipment):
MagMAX™ Express-96 Magnetic Particle Processor, KingFisher 96, MagMAX™ Express-96 Standard Magnetic Particle Processor
No. of Reactions:
96
Scale:
Midi
Target:
Viral DNA/RNA
Content And Storage: Store Processing Plate with Lid, Lysis/Binding Solution Concentrate, Wash Solution 1 Concentrate, Wash Solution 2 Concentrate, and Elution Buffer at room temp.Store MagMax Turbo DNase Buffer and RNA Binding Beads at 4°C.Store Lysis/Binding Enhancer and TURBO DNase at -20°C.
Isolation Technology: Magnetic Bead
Sample Type: Liquid Samples (e.g. Serum), Milk, Viral Samples, Whole Blood (Mammalian)
High-throughput Compatibility: High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols
For Use With (Application): Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Product Line: Ambion™, MagMAX™
Starting Material Amount: Up to 50 μl whole blood
Yield: 0.5 μg
Format: 96-well Plate
Purification Time: __
Final Product Type: Total RNA, Viral RNA
For Use With (Equipment): MagMAX™ Express 24 and 96, Robotic Liquid Handling Systems, MagMAX™ Express-96 Standard Magnetic Particle Processor
No. of Reactions: 96 Preps
Scale: __
Target: Viral DNA/RNA, Total RNA
Content And Storage:
Store Processing Plate with Lid, Lysis/Binding Solution Concentrate, Wash Solution 1 Concentrate, Wash Solution 2 Concentrate, and Elution Buffer at room temp.Store MagMax Turbo DNase Buffer and RNA Binding Beads at 4°C.Store Lysis/Binding Enhancer and TURBO DNase at -20°C.
Isolation Technology:
Magnetic Bead
Sample Type:
Liquid Samples (e.g. Serum), Milk, Viral Samples, Whole Blood (Mammalian)
High-throughput Compatibility:
High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols
For Use With (Application):
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Product Line:
Ambion™, MagMAX™
Starting Material Amount:
Up to 50 μl whole blood
Yield:
0.5 μg
Format:
96-well Plate
Purification Time:
__
Final Product Type:
Total RNA, Viral RNA
For Use With (Equipment):
MagMAX™ Express 24 and 96, Robotic Liquid Handling Systems, MagMAX™ Express-96 Standard Magnetic Particle Processor
No. of Reactions:
96 Preps
Scale:
__
Target:
Viral DNA/RNA, Total RNA
Content And Storage: Kit Contents (storage):• 1 Processing Plate & Lid (room temperature)• 12 mL Lysis/Binding Solution Concentrate (room temperature)• 18 mL Wash Solution 1 Concentrate (room temperature)• 72.5 mL Wash Solution 2 Concentrate (room temperature)• 14 mL Elution Buffer (room temperature)• 6 mL MagMAX™ TURBO™ DNase Buffer (4°C or room temperature)• 4 x 25 mL TRI Reagent™ (4°C)• 1.1 mL RNA Binding Beads (4°C)• 1.1 mL Lysis/Binding Enhancer (-20°C)• 215 μL TURBO™ DNase, 10 U/μL (-20°C)Contains enough reagents to isolate RNA from 96 samples.
Isolation Technology: Magnetic Bead, Organic Extraction
Sample Type: Cells, Mammalian Cells, Plant Samples, Tissue (Mammalian), Yeast
High-throughput Compatibility: High-throughput Compatible, Automated Protocols
For Use With (Application): Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Product Line: Ambion™, MagMAX™
Starting Material Amount: Up to 5 x10^6 cells, Up to 100 mg tissue
Yield: __
Format: 96-well Plate
Purification Time: <1 hr.
Final Product Type: Total RNA
For Use With (Equipment): MagMAX™ Express 24 and 96, Robotic Liquid Handling Systems, MagMAX™ Express-96 Standard Magnetic Particle Processor
No. of Reactions: 96 Preps
Scale: __
Target: Total RNA
Content And Storage:
Kit Contents (storage):• 1 Processing Plate & Lid (room temperature)• 12 mL Lysis/Binding Solution Concentrate (room temperature)• 18 mL Wash Solution 1 Concentrate (room temperature)• 72.5 mL Wash Solution 2 Concentrate (room temperature)• 14 mL Elution Buffer (room temperature)• 6 mL MagMAX™ TURBO™ DNase Buffer (4°C or room temperature)• 4 x 25 mL TRI Reagent™ (4°C)• 1.1 mL RNA Binding Beads (4°C)• 1.1 mL Lysis/Binding Enhancer (-20°C)• 215 μL TURBO™ DNase, 10 U/μL (-20°C)Contains enough reagents to isolate RNA from 96 samples.
Isolation Technology:
Magnetic Bead, Organic Extraction
Sample Type:
Cells, Mammalian Cells, Plant Samples, Tissue (Mammalian), Yeast
High-throughput Compatibility:
High-throughput Compatible, Automated Protocols
For Use With (Application):
Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Product Line:
Ambion™, MagMAX™
Starting Material Amount:
Up to 5 x10^6 cells, Up to 100 mg tissue
Yield:
__
Format:
96-well Plate
Purification Time:
<1 hr.
Final Product Type:
Total RNA
For Use With (Equipment):
MagMAX™ Express 24 and 96, Robotic Liquid Handling Systems, MagMAX™ Express-96 Standard Magnetic Particle Processor
No. of Reactions:
96 Preps
Scale:
__
Target:
Total RNA
Content And Storage: • Carrier NA and Lysis/Binding Enhancer, store at -20°C• RNA Binding Beads, 4°C• Wash Solution 2 Concentrate, 4°C or room temperature• Elution Buffer, 4°C or room temperature• Lysis/Binding Solution Concentrate, room temperature• Wash Solution 1 Concentrate, room temperature• Processing Plates, room temperature• Lids, room temperature• PBS Buffer, room temperature
Isolation Technology: Magnetic Bead
Sample Type: Bacteria, Blood, Cells, Liquid Samples (e.g. Serum)
High-throughput Compatibility: High-throughput Compatible
For Use With (Application): Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Southern Blotting, PCR, Northern Blotting
Product Line: MagMAX™
Starting Material Amount: Up to 0.3 g solid samples or 175 μl liquid samples
Yield: 420 ng
Format: 96-well Plate
Purification Time: __
Final Product Type: RNA, DNA
For Use With (Equipment): __
No. of Reactions: __
Scale: __
Target: Total RNA, Total Nucleic Acids, Genomic DNA
Content And Storage:
• Carrier NA and Lysis/Binding Enhancer, store at -20°C• RNA Binding Beads, 4°C• Wash Solution 2 Concentrate, 4°C or room temperature• Elution Buffer, 4°C or room temperature• Lysis/Binding Solution Concentrate, room temperature• Wash Solution 1 Concentrate, room temperature• Processing Plates, room temperature• Lids, room temperature• PBS Buffer, room temperature
Isolation Technology:
Magnetic Bead
Sample Type:
Bacteria, Blood, Cells, Liquid Samples (e.g. Serum)
High-throughput Compatibility:
High-throughput Compatible
For Use With (Application):
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Southern Blotting, PCR, Northern Blotting
Product Line:
MagMAX™
Starting Material Amount:
Up to 0.3 g solid samples or 175 μl liquid samples
Yield:
420 ng
Format:
96-well Plate
Purification Time:
__
Final Product Type:
RNA, DNA
For Use With (Equipment):
__
No. of Reactions:
__
Scale:
__
Target:
Total RNA, Total Nucleic Acids, Genomic DNA