AM1837
Invitrogen™ MagMAX™-96 Blood RNA Isolation Kit
Manufacturer: Invitrogen™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | AM1837-Each-of-1 | In Stock | ₹ 64,032.00 |
AM1837 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 64,032.00
GST (18%): ₹ 11,525.76
Total Price: ₹ 75,557.76
Content And Storage
Store Processing Plate with Lid, Lysis/Binding Solution Concentrate, Wash Solution 1 Concentrate, Wash Solution 2 Concentrate, and Elution Buffer at room temp.Store MagMax Turbo DNase Buffer and RNA Binding Beads at 4°C.Store Lysis/Binding Enhancer and TURBO DNase at -20°C.
Isolation Technology
Magnetic Bead
Final Product Type
Total RNA, Viral RNA
For Use With (Equipment)
MagMAX™ Express 24 and 96, Robotic Liquid Handling Systems, MagMAX™ Express-96 Standard Magnetic Particle Processor
No. of Reactions
96 Preps
Product Line
Ambion™, MagMAX™
Target
Viral DNA/RNA, Total RNA
Format
96-well Plate
Sample Type
Liquid Samples (e.g. Serum), Milk, Viral Samples, Whole Blood (Mammalian)
High-throughput Compatibility
High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols
For Use With (Application)
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Prep Scale
< 100 μL Blood Samples (Small-scale)
Starting Material Amount
Up to 50 μl whole blood
Yield
0.5 μg
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Description
- Description This Ambion™ kit is for the rapid, high-throughput purification of pathogen RNA from mammalian whole blood, milk, and other biofluids in 96-well plates
- RNA purified using the kit is ideal for RT-PCR applications
- The kit uses magnetic bead–based purification and includes sufficient reagents for 96 reactions
- • Easily isolate viral or total RNA from whole blood • Save precious time—complete the protocol in less than 1 hour • Walk away—integrate with established robotic platforms • Validated with human, bovine, porcine, and ovine whole blood Benefits of MagMAX™ Magnetic Bead-Based Purification: Magnetic beads offer many benefits compared to other technologies for isolating RNA
- Beads bind RNA more efficiently than glass fiber filters, resulting in higher and more consistent RNA yields
- Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples
- Since only a small volume of magnetic beads is needed for high- efficiency binding, the bound RNA can be eluted in just 30–50 μL of nuclease-free water, concentrating RNA from large, dilute samples
- Isolate RNA from as Little as 50 μl of Blood: For the highest-quality RNA, use whole blood samples that have not been frozen
- Approximately 300–500 ng of intact RNA can be consistently purified from 50 μl of human whole blood
- Bovine, porcine, and ovine whole blood samples can also be used
- Yields vary from species to species (see data)
- The recovered RNA is devoid of PCR inhibitors such as proteins and heme, commonly found in RNA isolated from blood
- Isolate Viral and Total RNA from Whole Blood Samples: The MagMAX™-96 Blood RNA Isolation Kit eliminates sample preprocessing, which can cause sample mix-up and cross-contamination
- The procedure is fast (less than 1 hour), simple, and well suited for automation
- The kit can also be used to process fewer than 96 samples at one time and is compatible with either manual processing using multi-channel pipettors or processing with robotic liquid handlers
- Downloadable protocols for the use of this kit with specific liquid handling systems are available at our Automation Resource
- The MagMAX™-96 Blood RNA Isolation Kit can also be used for total nucleic acid isolation by omitting the DNase treatment.
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Content And Storage: Store Processing Plate with Lid, Lysis/Binding Solution Concentrate, Wash Solution 1 Concentrate, Wash Solution 2 Concentrate, and Elution Buffer at room temp.Store MagMax Turbo DNase Buffer and RNA Binding Beads at 4°C.Store Lysis/Binding Enhancer and TURBO DNase at -20°C.
Isolation Technology: Magnetic Bead
Final Product Type: Total RNA, Viral RNA
For Use With (Equipment): MagMAX™ Express 24 and 96, Robotic Liquid Handling Systems, MagMAX™ Express-96 Standard Magnetic Particle Processor
No. of Reactions: 96 Preps
Product Line: Ambion™, MagMAX™
Target: Viral DNA/RNA, Total RNA
Format: 96-well Plate
Sample Type: Liquid Samples (e.g. Serum), Milk, Viral Samples, Whole Blood (Mammalian)
High-throughput Compatibility: High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols
For Use With (Application): Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Prep Scale: < 100 μL Blood Samples (Small-scale)
Starting Material Amount: Up to 50 μl whole blood
Yield: 0.5 μg
Content And Storage:
Store Processing Plate with Lid, Lysis/Binding Solution Concentrate, Wash Solution 1 Concentrate, Wash Solution 2 Concentrate, and Elution Buffer at room temp.Store MagMax Turbo DNase Buffer and RNA Binding Beads at 4°C.Store Lysis/Binding Enhancer and TURBO DNase at -20°C.
Isolation Technology:
Magnetic Bead
Final Product Type:
Total RNA, Viral RNA
For Use With (Equipment):
MagMAX™ Express 24 and 96, Robotic Liquid Handling Systems, MagMAX™ Express-96 Standard Magnetic Particle Processor
No. of Reactions:
96 Preps
Product Line:
Ambion™, MagMAX™
Target:
Viral DNA/RNA, Total RNA
Format:
96-well Plate
Sample Type:
Liquid Samples (e.g. Serum), Milk, Viral Samples, Whole Blood (Mammalian)
High-throughput Compatibility:
High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols
For Use With (Application):
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Prep Scale:
< 100 μL Blood Samples (Small-scale)
Starting Material Amount:
Up to 50 μl whole blood
Yield:
0.5 μg
Content And Storage: Kit Contents (storage):• 1 Processing Plate & Lid (room temperature)• 12 mL Lysis/Binding Solution Concentrate (room temperature)• 18 mL Wash Solution 1 Concentrate (room temperature)• 72.5 mL Wash Solution 2 Concentrate (room temperature)• 14 mL Elution Buffer (room temperature)• 6 mL MagMAX™ TURBO™ DNase Buffer (4°C or room temperature)• 4 x 25 mL TRI Reagent™ (4°C)• 1.1 mL RNA Binding Beads (4°C)• 1.1 mL Lysis/Binding Enhancer (-20°C)• 215 μL TURBO™ DNase, 10 U/μL (-20°C)Contains enough reagents to isolate RNA from 96 samples.
Isolation Technology: Magnetic Bead, Organic Extraction
Final Product Type: Total RNA
For Use With (Equipment): MagMAX™ Express 24 and 96, Robotic Liquid Handling Systems, MagMAX™ Express-96 Standard Magnetic Particle Processor
No. of Reactions: 96 Preps
Product Line: Ambion™, MagMAX™
Target: Total RNA
Format: 96-well Plate
Sample Type: Cells, Mammalian Cells, Plant Samples, Tissue (Mammalian), Yeast
High-throughput Compatibility: High-throughput Compatible, Automated Protocols
For Use With (Application): Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Prep Scale: __
Starting Material Amount: Up to 5 x10^6 cells, Up to 100 mg tissue
Yield: __
Content And Storage:
Kit Contents (storage):• 1 Processing Plate & Lid (room temperature)• 12 mL Lysis/Binding Solution Concentrate (room temperature)• 18 mL Wash Solution 1 Concentrate (room temperature)• 72.5 mL Wash Solution 2 Concentrate (room temperature)• 14 mL Elution Buffer (room temperature)• 6 mL MagMAX™ TURBO™ DNase Buffer (4°C or room temperature)• 4 x 25 mL TRI Reagent™ (4°C)• 1.1 mL RNA Binding Beads (4°C)• 1.1 mL Lysis/Binding Enhancer (-20°C)• 215 μL TURBO™ DNase, 10 U/μL (-20°C)Contains enough reagents to isolate RNA from 96 samples.
Isolation Technology:
Magnetic Bead, Organic Extraction
Final Product Type:
Total RNA
For Use With (Equipment):
MagMAX™ Express 24 and 96, Robotic Liquid Handling Systems, MagMAX™ Express-96 Standard Magnetic Particle Processor
No. of Reactions:
96 Preps
Product Line:
Ambion™, MagMAX™
Target:
Total RNA
Format:
96-well Plate
Sample Type:
Cells, Mammalian Cells, Plant Samples, Tissue (Mammalian), Yeast
High-throughput Compatibility:
High-throughput Compatible, Automated Protocols
For Use With (Application):
Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Prep Scale:
__
Starting Material Amount:
Up to 5 x10^6 cells, Up to 100 mg tissue
Yield:
__
Content And Storage: • Carrier NA and Lysis/Binding Enhancer, store at -20°C• RNA Binding Beads, 4°C• Wash Solution 2 Concentrate, 4°C or room temperature• Elution Buffer, 4°C or room temperature• Lysis/Binding Solution Concentrate, room temperature• Wash Solution 1 Concentrate, room temperature• Processing Plates, room temperature• Lids, room temperature• PBS Buffer, room temperature
Isolation Technology: Magnetic Bead
Final Product Type: RNA, DNA
For Use With (Equipment): __
No. of Reactions: __
Product Line: MagMAX™
Target: Total RNA, Total Nucleic Acids, Genomic DNA
Format: 96-well Plate
Sample Type: Bacteria, Blood, Cells, Liquid Samples (e.g. Serum)
High-throughput Compatibility: High-throughput Compatible
For Use With (Application): Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Southern Blotting, PCR, Northern Blotting
Prep Scale: __
Starting Material Amount: Up to 0.3 g solid samples or 175 μl liquid samples
Yield: 420 ng
Content And Storage:
• Carrier NA and Lysis/Binding Enhancer, store at -20°C• RNA Binding Beads, 4°C• Wash Solution 2 Concentrate, 4°C or room temperature• Elution Buffer, 4°C or room temperature• Lysis/Binding Solution Concentrate, room temperature• Wash Solution 1 Concentrate, room temperature• Processing Plates, room temperature• Lids, room temperature• PBS Buffer, room temperature
Isolation Technology:
Magnetic Bead
Final Product Type:
RNA, DNA
For Use With (Equipment):
__
No. of Reactions:
__
Product Line:
MagMAX™
Target:
Total RNA, Total Nucleic Acids, Genomic DNA
Format:
96-well Plate
Sample Type:
Bacteria, Blood, Cells, Liquid Samples (e.g. Serum)
High-throughput Compatibility:
High-throughput Compatible
For Use With (Application):
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Southern Blotting, PCR, Northern Blotting
Prep Scale:
__
Starting Material Amount:
Up to 0.3 g solid samples or 175 μl liquid samples
Yield:
420 ng
Content And Storage: Store Control RNA, Capture Oligo Mix, Glycogen, and 3M Sodium Acetate at -20°C.Store Oligo MagBeads, Binding Buffer, and Wash Solution at 4°CStore Nuclease-free Water, 1.5mL tubes, and Collection tubes at room temperature.
Isolation Technology: Hybridization Capture, Magnetic Bead
Final Product Type: mRNA (Bacterial)
For Use With (Equipment): __
No. of Reactions: 20 Preps
Product Line: Ambion™, MICROBEnrich™
Target: Total RNA
Format: __
Sample Type: Total RNA (Bacterial)
High-throughput Compatibility: Not High-throughput Compatible (Manual)
For Use With (Application): Microarray Analysis, Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction, Northern Blotting
Prep Scale: __
Starting Material Amount: __
Yield: __
Content And Storage:
Store Control RNA, Capture Oligo Mix, Glycogen, and 3M Sodium Acetate at -20°C.Store Oligo MagBeads, Binding Buffer, and Wash Solution at 4°CStore Nuclease-free Water, 1.5mL tubes, and Collection tubes at room temperature.
Isolation Technology:
Hybridization Capture, Magnetic Bead
Final Product Type:
mRNA (Bacterial)
For Use With (Equipment):
__
No. of Reactions:
20 Preps
Product Line:
Ambion™, MICROBEnrich™
Target:
Total RNA
Format:
__
Sample Type:
Total RNA (Bacterial)
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
For Use With (Application):
Microarray Analysis, Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction, Northern Blotting
Prep Scale:
__
Starting Material Amount:
__
Yield:
__