AMB18365

Applied Biosystems™ MagMAX™-96 Viral RNA Isolation Kit

Manufacturer: Fischer Scientific

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Pack Size SKU Availability Price
Each of 1 AMB18365-Each-of-1 In Stock ₹ 1,81,815.00

AMB18365 - Each of 1

₹ 1,81,815.00

In Stock

Quantity

1

Base Price: ₹ 1,81,815.00

GST (18%): ₹ 32,726.70

Total Price: ₹ 2,14,541.70

Content And Storage

Carrier RNA and Lysis Binding Enhancer is stored at –20°C; RNA Binding Beads are stored at 4°C. Wash Solution 2 Concentrate and Elution Buffer may be stored at either 4°C or room temperature. Lysis⁄Binding Solution Concentrate, Wash Solution 1 Concentrate, and Processing Plates and Lids are stored at room temperature. Nuclease-free water may be stored at any temperature.

Isolation Technology

Magnetic Bead

Sample Type

Cell-free Biological Fluids, Liquid Samples (e.g. Serum), Viral Samples

High-throughput Compatibility

High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols

For Use With (Application)

Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting

Product Line

MagMAX™

Scale

Maxi

Target

Viral DNA/RNA

Format

96-well Plate

Purification Time

45 min.

Final Product Type

Viral DNA⁄RNA

For Use With (Equipment)

KingFisher™ Flex Magnetic Particle Processor, KingFisher 96

No. of Reactions

480

Quantity

5 x 96 Preps

Starting Material Amount

Up to 50 μl

Yield

5 ng

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Description

  • Perfect for working with low viral concentrations, the kit contains sufficient reagents for 5 × 96 reactions
  • Benefits of MagMAX™ Magnetic Bead-Based Purification: Magnetic beads offer many benefits compared to other technologies for isolating RNA
  • Beads bind RNA more efficiently than glass fiber filters, resulting in higher and more consistent RNA yields
  • Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples
  • Since only a small volume of magnetic beads is needed for high-efficiency binding, the bound RNA can be eluted in just 20–50μL of nuclease-free water, concentrating RNA from large, dilute samples
  • Optimized for Isolation of Virus from Biofluids: The Applied Biosystems™ MagMAX™ Viral RNA Isolation Kits have been optimized for use with biological fluids and cell-free samples such as serum, plasma, swabs, and cell culture media
  • These samples often present unique challenges when purifying RNA, including low viral titers, large volumes to be processed, and sample complexity
  • Without the need for organic solvents or RNA precipitation, the MagMAX™ Viral RNA Isolation Kit eliminates the problems often encountered with other RNA isolation methods such filter clogging, large elution volumes, and inconsistent yields
  • With this kit, DNA and RNA input levels ranging from 20 copies to 25 x 10 6 copies have been quantitatively recovered from plasma, serum, and milk
  • And even with less sample input, pathogen detection was as sensitive (equivalent C Ts ) or more sensitive (lower C Ts ) using nucleic acid isolated with the MagMAX™ kit when compared to nucleic acid isolated using another company's product
  • Downloadable protocols for the use of this kit with specific liquid handling systems are available at our Automation Resource
  • Accessory Products: Carrier RNA (SKU#4382878), Lysis/Binding Solution Concentrate (SKU#AM8500), Wash Solution 1 Concentrate (SKU#AM8504), and Wash Solution 2 Concentrate (SKU#AM8640) are components of the MagMAX™ Viral RNA Isolation Kits that may also be purchased separately
  • A 96-well magnetic ring stand is required to use this kit
  • The Magnetic Stand-96 (SKU#AM10027) and the 96-well Magnetic Ring Stand (SKU#AM10050) are designed for paramagnetic bead precipitation from standard 96- well, U-bottom microplates
  • The Magnetic-Ring Stand pellets the magnetic beads in a circle shape, enabling easy removal of supernatant by a robot or multichannel pipettors
  • For additional processing plates, Matrix 96-Well U Bottom Polypropylene Plates (Thermo Scientific, Item# 4917) are recommended
  • For an NVSL-validated version of this kit, please see the MagMAX™-96 AI/ND Viral RNA Isolation Kits (SKU#1929 and SKU#AM1835).

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Content And Storage:
Carrier RNA and Lysis Binding Enhancer is stored at –20°C; RNA Binding Beads are stored at 4°C. Wash Solution 2 Concentrate and Elution Buffer may be stored at either 4°C or room temperature. Lysis⁄Binding Solution Concentrate, Wash Solution 1 Concentrate, and Processing Plates and Lids are stored at room temperature. Nuclease-free water may be stored at any temperature.

Isolation Technology:
Magnetic Bead

Sample Type:
Cell-free Biological Fluids, Liquid Samples (e.g. Serum), Viral Samples

High-throughput Compatibility:
High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols

For Use With (Application):
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting

Product Line:
MagMAX™

Scale:
Maxi

Target:
Viral DNA/RNA

Format:
96-well Plate

Purification Time:
45 min.

Final Product Type:
Viral DNA⁄RNA

For Use With (Equipment):
KingFisher™ Flex Magnetic Particle Processor, KingFisher 96

No. of Reactions:
480

Quantity:
5 x 96 Preps

Starting Material Amount:
Up to 50 μl

Yield:
5 ng

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Quantity:
500 μg

Starting Material Amount:
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Yield:
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Sample Type:
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High-throughput Compatibility:
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For Use With (Application):
__

Product Line:
__

Scale:
__

Target:
__

Format:
__

Purification Time:
__

Final Product Type:
__

For Use With (Equipment):
__

No. of Reactions:
__

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500 μg

Starting Material Amount:
__

Yield:
__

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-20°C

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__

Sample Type:
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High-throughput Compatibility:
__

For Use With (Application):
__

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__

Scale:
__

Target:
__

Format:
__

Purification Time:
__

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For Use With (Equipment):
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No. of Reactions:
__

Quantity:
500 μg

Starting Material Amount:
__

Yield:
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