Invitrogen™ Mouse RiboPure™-Blood RNA Isolation Kit with Manual

Description

• For the purification of extremely high-quality total RNA (and optionally, microRNA) from mouse or rat blood in about 35 minutes
• The Ambion™ kit includes sufficient reagents for 25 RNA purifications from whole blood samples ranging from 0.1–0.5 each
• • Includes RNA later ™, predispensed in 2 mL tubes, for RNA stabilization • Resulting RNA is suitable for stringent downstream applications such as array analysis and qRT-PCR Due to interference from the enzymatic inhibitors and anticoagulants present in blood samples, RNA purification from whole blood requires a stringent isolation method that yields high-quality RNA depleted of inhibiting agents and contaminants
• The kit includes microfuge tubes preloaded with RNA later ™ Tissue Collection:RNA Stabilization Solution, an aqueous, nontoxic storage reagent that prevents RNA degradation and preserves RNA profiles within samples
• Stabilized samples can be stored at room temperature for up to 3 days or at –20°C for long-term storage
• The related Figures show the performance of RNA isolated with the Mouse RiboPure™-Blood Kit in real-time RT-PCR
• The kit includes an alternative protocol for isolating total RNA that contains microRNA
• Typically, 30–55 μg of total RNA (containing microRNA) is obtained from 0.5 mL mouse blood
• Yields from rat blood are considerably more variable, generally 18–40 μg from 0.5 mL blood
• Order Info Shipping Condition: Room temperature