AM1556
Invitrogen™mirVana™ PARIS™ RNA and Native Protein Purification Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | AM1556-Each-of-1 | In Stock | ₹ 44,620.00 |
AM1556 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 44,620.00
GST (18%): ₹ 8,031.60
Total Price: ₹ 52,651.60
Content And Storage
• 30 mL miRNA Wash Solution I (room temperature)• 50 mL Wash Solution 2/3 (room temperature)• 80 Collection Tubes (room temperature)• 40 Filter Cartridges (room temperature)• 25 mL Cell Disruption Buffer (4°C)• 25 mL 2X Denaturing Solution (4°C)• 2 × 25 mL Acid-Phenol:Chloroform (4°C)• 5 mL Elution Solution (-20°C, 4°C, or room temperature)
Purification Time
30 min.
Final Product Type
Total RNA, Transcriptome RNA, siRNA, Protein (Native), snRNA, micro RNA
For Use With (Application)
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Western Blotting, microRNA Analysis, Northern Blotting, cDNA Library Construction
Product Line
mirVana™, Ambion™
Starting Material Amount
Up to 10^7 cells, Up to 100 mg tissue
Isolation Technology
Organic Extraction, Cell Lysis (Nonionic Detergent), Spin Column (Glass Fiber Filter)
Sample Type
Cell Cultures, Tissue (Mammalian)
High-throughput Compatibility
Not High-throughput Compatible (Manual)
No. of Reactions
40 Preps
Shipping Condition
Room Temperature
Target
Total RNA, RNA and Protein, siRNA, miRNA
Related Products
Description
- Description The mir Vana™ PARIS™ Kit is for the purification of both native protein and RNA (including small RNA) from the same sample
- Each kit contains sufficient reagents and consumables for either 40 isolations of protein and total RNA (including small RNAs), or 20 isolations of protein and separate large and small RNA fractions
- How the kit works The mir Vana PARIS Kit procedure begins with homogenization of samples with a special cell disruption buffer that includes nonionic detergent
- Protein remains intact, so a portion of the lysate can be used directly for common protein analysis applications
- The remainder of the lysate is used for RNA isolation, using a procedure that combines the advantages of organic and solid-phase extraction, while avoiding the disadvantages of both
- Isolate small RNA-containing total RNA and native protein from the same sample Start with 100–10 7 cultured cells or up to 100 mg of many mammalian tissues Enrich for small RNA (<200 nt) to increase sensitivity in downstream analyses Follow a simple, 30-minute procedure Use to correlate mRNA, miRNA or siRNA, and protein levels Not high-throughput compatible (Manual) Order Info Shipping Condition: Room temperature
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Content And Storage: • 30 mL miRNA Wash Solution I (room temperature)• 50 mL Wash Solution 2/3 (room temperature)• 80 Collection Tubes (room temperature)• 40 Filter Cartridges (room temperature)• 25 mL Cell Disruption Buffer (4°C)• 25 mL 2X Denaturing Solution (4°C)• 2 × 25 mL Acid-Phenol:Chloroform (4°C)• 5 mL Elution Solution (-20°C, 4°C, or room temperature)
Purification Time: 30 min.
Final Product Type: Total RNA, Transcriptome RNA, siRNA, Protein (Native), snRNA, micro RNA
For Use With (Application): Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Western Blotting, microRNA Analysis, Northern Blotting, cDNA Library Construction
Product Line: mirVana™, Ambion™
Starting Material Amount: Up to 10^7 cells, Up to 100 mg tissue
Isolation Technology: Organic Extraction, Cell Lysis (Nonionic Detergent), Spin Column (Glass Fiber Filter)
Sample Type: Cell Cultures, Tissue (Mammalian)
High-throughput Compatibility: Not High-throughput Compatible (Manual)
No. of Reactions: 40 Preps
Shipping Condition: Room Temperature
Target: Total RNA, RNA and Protein, siRNA, miRNA
Content And Storage:
• 30 mL miRNA Wash Solution I (room temperature)• 50 mL Wash Solution 2/3 (room temperature)• 80 Collection Tubes (room temperature)• 40 Filter Cartridges (room temperature)• 25 mL Cell Disruption Buffer (4°C)• 25 mL 2X Denaturing Solution (4°C)• 2 × 25 mL Acid-Phenol:Chloroform (4°C)• 5 mL Elution Solution (-20°C, 4°C, or room temperature)
Purification Time:
30 min.
Final Product Type:
Total RNA, Transcriptome RNA, siRNA, Protein (Native), snRNA, micro RNA
For Use With (Application):
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Western Blotting, microRNA Analysis, Northern Blotting, cDNA Library Construction
Product Line:
mirVana™, Ambion™
Starting Material Amount:
Up to 10^7 cells, Up to 100 mg tissue
Isolation Technology:
Organic Extraction, Cell Lysis (Nonionic Detergent), Spin Column (Glass Fiber Filter)
Sample Type:
Cell Cultures, Tissue (Mammalian)
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
No. of Reactions:
40 Preps
Shipping Condition:
Room Temperature
Target:
Total RNA, RNA and Protein, siRNA, miRNA
Content And Storage: • 30 mL miRNA Wash Solution I (room temperature)• 50 mL Wash Solution 2/3 (room temperature)• 80 Collection Tubes (room temperature)• 40 Filter Cartridges (room temperature)• 100 mL Lysis/Binding Buffer (4°C)• 10 mL miRNA Homogenate Additive (4°C)• 100 mL Acid-Phenol:Chloroform (4°C)• 1.4 mL Gel Loading Buffer II (-20°C)• 5 mL Elution Solution (-20°C, 4°C, or room temperature)
Purification Time: 30 min. (After sample homogenization)
Final Product Type: Small RNA
For Use With (Application): qRT-PCR, microarray analysis, Northern blotting
Product Line: __
Starting Material Amount: Bacteria: ≤107cellsCells: ≤107Plant: ≤250 mgTissue: ≤250 mgVirus: ≤107cellsYeast: ≤107cellsEnzymatic Reactions: varies
Isolation Technology: Spin Column (Glass Fiber Filter), Organic Extraction
Sample Type: Bacteria, Cells, Plant, Tissue, Viruses, Yeast, Enzymatic Reactions
High-throughput Compatibility: Not High-throughput Compatible (Manual)
No. of Reactions: __
Shipping Condition: Room Temperature
Target: __
Content And Storage:
• 30 mL miRNA Wash Solution I (room temperature)• 50 mL Wash Solution 2/3 (room temperature)• 80 Collection Tubes (room temperature)• 40 Filter Cartridges (room temperature)• 100 mL Lysis/Binding Buffer (4°C)• 10 mL miRNA Homogenate Additive (4°C)• 100 mL Acid-Phenol:Chloroform (4°C)• 1.4 mL Gel Loading Buffer II (-20°C)• 5 mL Elution Solution (-20°C, 4°C, or room temperature)
Purification Time:
30 min. (After sample homogenization)
Final Product Type:
Small RNA
For Use With (Application):
qRT-PCR, microarray analysis, Northern blotting
Product Line:
__
Starting Material Amount:
Bacteria: ≤107cellsCells: ≤107Plant: ≤250 mgTissue: ≤250 mgVirus: ≤107cellsYeast: ≤107cellsEnzymatic Reactions: varies
Isolation Technology:
Spin Column (Glass Fiber Filter), Organic Extraction
Sample Type:
Bacteria, Cells, Plant, Tissue, Viruses, Yeast, Enzymatic Reactions
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
No. of Reactions:
__
Shipping Condition:
Room Temperature
Target:
__
Content And Storage: • 30 mL miRNA Wash Solution I (room temperature)• 50 mL Wash Solution 2/3 (room temperature)• 80 Collection Tubes (room temperature)• 40 Filter Cartridges (room temperature)• 100 mL Lysis/Binding Buffer (4°C)• 10 mL miRNA Homogenate Additive (4°C)• 1.4 mL Gel Loading Buffer II (-20°C)• 5 mL Elution Solution (-20°C, 4°C, or room temperature)
Purification Time: 30 min. (After sample homogenization)
Final Product Type: Small RNA
For Use With (Application): qRT-PCR, microarray analysis, Northern blotting
Product Line: __
Starting Material Amount: Bacteria: ≤107cellsCells: ≤107Plant: ≤250 mgTissue: ≤250 mgVirus: ≤107cellsYeast: ≤107cellsEnzymatic Reactions: varies
Isolation Technology: Spin Column (Glass Fiber Filter), Organic Extraction
Sample Type: Bacteria, Cells, Plant, Tissue, Viruses, Yeast, Enzymatic Reactions
High-throughput Compatibility: Not High-throughput Compatible (Manual)
No. of Reactions: __
Shipping Condition: Room Temperature
Target: __
Content And Storage:
• 30 mL miRNA Wash Solution I (room temperature)• 50 mL Wash Solution 2/3 (room temperature)• 80 Collection Tubes (room temperature)• 40 Filter Cartridges (room temperature)• 100 mL Lysis/Binding Buffer (4°C)• 10 mL miRNA Homogenate Additive (4°C)• 1.4 mL Gel Loading Buffer II (-20°C)• 5 mL Elution Solution (-20°C, 4°C, or room temperature)
Purification Time:
30 min. (After sample homogenization)
Final Product Type:
Small RNA
For Use With (Application):
qRT-PCR, microarray analysis, Northern blotting
Product Line:
__
Starting Material Amount:
Bacteria: ≤107cellsCells: ≤107Plant: ≤250 mgTissue: ≤250 mgVirus: ≤107cellsYeast: ≤107cellsEnzymatic Reactions: varies
Isolation Technology:
Spin Column (Glass Fiber Filter), Organic Extraction
Sample Type:
Bacteria, Cells, Plant, Tissue, Viruses, Yeast, Enzymatic Reactions
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
No. of Reactions:
__
Shipping Condition:
Room Temperature
Target:
__
Content And Storage: DNA Hybridization Buffer, T7 Promoter Primer, Klenow Reaction Buffer, 10X dNTP, Exo– Klenow, T7 Enzyme Mix, T7 Reaction Buffer, 2X NTP Mix, Digestion Buffer, DNase, RNase, Sense Control DNA, and Antisense Control DNA should be stored at –20°C. siRNA Binding Buffer, siRNA Wash Buffer, Filter Cartridges, and Tubes should be stored at room temperature. Nuclease-free Water may be stored at any temperature.
Purification Time: __
Final Product Type: __
For Use With (Application): __
Product Line: Silencer™, Ambion™
Starting Material Amount: __
Isolation Technology: __
Sample Type: In vitro Transcribed siRNA
High-throughput Compatibility: __
No. of Reactions: 15
Shipping Condition: Dry Ice
Target: __
Content And Storage:
DNA Hybridization Buffer, T7 Promoter Primer, Klenow Reaction Buffer, 10X dNTP, Exo– Klenow, T7 Enzyme Mix, T7 Reaction Buffer, 2X NTP Mix, Digestion Buffer, DNase, RNase, Sense Control DNA, and Antisense Control DNA should be stored at –20°C. siRNA Binding Buffer, siRNA Wash Buffer, Filter Cartridges, and Tubes should be stored at room temperature. Nuclease-free Water may be stored at any temperature.
Purification Time:
__
Final Product Type:
__
For Use With (Application):
__
Product Line:
Silencer™, Ambion™
Starting Material Amount:
__
Isolation Technology:
__
Sample Type:
In vitro Transcribed siRNA
High-throughput Compatibility:
__
No. of Reactions:
15
Shipping Condition:
Dry Ice
Target:
__