AM1561
Invitrogen™mirVana™ miRNA Isolation Kit, without phenol
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | AM1561-Each-of-1 | In Stock | ₹ 36,248.00 |
AM1561 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 36,248.00
GST (18%): ₹ 6,524.64
Total Price: ₹ 42,772.64
Content And Storage
• 30 mL miRNA Wash Solution I (room temperature)• 50 mL Wash Solution 2/3 (room temperature)• 80 Collection Tubes (room temperature)• 40 Filter Cartridges (room temperature)• 100 mL Lysis/Binding Buffer (4°C)• 10 mL miRNA Homogenate Additive (4°C)• 1.4 mL Gel Loading Buffer II (-20°C)• 5 mL Elution Solution (-20°C, 4°C, or room temperature)
Purification Time
30 min. (After sample homogenization)
Sample Type
Bacteria, Cells, Plant, Tissue, Viruses, Yeast, Enzymatic Reactions
High-throughput Compatibility
Not High-throughput Compatible (Manual)
Quantity
40 Preps
Starting Material Amount
Bacteria: ≤107cellsCells: ≤107Plant: ≤250 mgTissue: ≤250 mgVirus: ≤107cellsYeast: ≤107cellsEnzymatic Reactions: varies
Isolation Technology
Spin Column (Glass Fiber Filter), Organic Extraction
Elution Volume
100 μL
Final Product Type
Small RNA
For Use With (Application)
qRT-PCR, microarray analysis, Northern blotting
Shipping Condition
Room Temperature
Yield
Cells: ≤16 μg RNA per 50,000 cellsTissue: ≤160 μg per 5 mg
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Description
- Description The mir Vana™ miRNA Isolation Kit uses a rapid procedure to isolate small RNAs from tissue and cells using an efficient glass fiber filter (GFF)-based method
- The method isolates total RNA ranging in size from kilobases down to 10-mers
- Each kit contains sufficient reagents and consumables for either 40 isolations of total RNA (including small RNAs), or 20 separate large and small RNA fractions
- Phenol must be purchased separately
- Features of this kit: • Efficient isolation of small RNA-containing total RNA • Enrich for small RNA (less than 200 nt) to increase sensitivity in downstream analyses • Simple 30 minute procedure • Ideal for miRNA, siRNA, shRNA, and snRNA analysis • Compatible with virtually all cell and tissue types Efficient recovery of small RNA Current RNA purification procedures rely on organic extraction, followed by alcohol precipitation or adsorption of nucleic acid molecules on a GFF or silicate matrix
- Unfortunately, the former procedure requires alcohol precipitation, which is time consuming and inefficiently recovers small RNAs
- The latter procedure typically results in the loss of substantial amounts of small RNA
- The mir Vana™ miRNA Isolation Kit uses organic extraction followed by purification on a GFF under specialized binding and wash conditions
- As a result, the kit effectively recovers all RNA—from large mRNA and ribosomal RNA down to 10-mers—from virtually all cell and tissue types
- The mir Vana™ miRNA Isolation Kit enables the isolation of small RNA-containing total RNA from samples consisting of 10 2 –10 7 cultured cells or 0.5–250 mg tissue
- Enrich for small RNA Although the mir Vana™ miRNA Isolation Kit efficiently purifies all RNA larger than 10 nt, it also includes procedures for isolating RNA fractions, specifically enriched or depleted in small RNA species
- Through the enrichment procedure included in the mir Vana™ miRNA Isolation Kit, RNA molecules of ∼200 nt and less can be efficiently purified away from the larger RNA species
- The resulting RNA preparation is highly enriched for miRNAs, siRNAs, and/or snRNAs
- Small RNA enrichment using the mir Vana™ miRNA Isolation Kit procedure allows more sensitive small RNA detection with less background as compared to the same assay used with total RNA.
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Content And Storage: • 30 mL miRNA Wash Solution I (room temperature)• 50 mL Wash Solution 2/3 (room temperature)• 80 Collection Tubes (room temperature)• 40 Filter Cartridges (room temperature)• 100 mL Lysis/Binding Buffer (4°C)• 10 mL miRNA Homogenate Additive (4°C)• 1.4 mL Gel Loading Buffer II (-20°C)• 5 mL Elution Solution (-20°C, 4°C, or room temperature)
Purification Time: 30 min. (After sample homogenization)
Sample Type: Bacteria, Cells, Plant, Tissue, Viruses, Yeast, Enzymatic Reactions
High-throughput Compatibility: Not High-throughput Compatible (Manual)
Quantity: 40 Preps
Starting Material Amount: Bacteria: ≤107cellsCells: ≤107Plant: ≤250 mgTissue: ≤250 mgVirus: ≤107cellsYeast: ≤107cellsEnzymatic Reactions: varies
Isolation Technology: Spin Column (Glass Fiber Filter), Organic Extraction
Elution Volume: 100 μL
Final Product Type: Small RNA
For Use With (Application): qRT-PCR, microarray analysis, Northern blotting
Shipping Condition: Room Temperature
Yield: Cells: ≤16 μg RNA per 50,000 cellsTissue: ≤160 μg per 5 mg
Content And Storage:
• 30 mL miRNA Wash Solution I (room temperature)• 50 mL Wash Solution 2/3 (room temperature)• 80 Collection Tubes (room temperature)• 40 Filter Cartridges (room temperature)• 100 mL Lysis/Binding Buffer (4°C)• 10 mL miRNA Homogenate Additive (4°C)• 1.4 mL Gel Loading Buffer II (-20°C)• 5 mL Elution Solution (-20°C, 4°C, or room temperature)
Purification Time:
30 min. (After sample homogenization)
Sample Type:
Bacteria, Cells, Plant, Tissue, Viruses, Yeast, Enzymatic Reactions
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
Quantity:
40 Preps
Starting Material Amount:
Bacteria: ≤107cellsCells: ≤107Plant: ≤250 mgTissue: ≤250 mgVirus: ≤107cellsYeast: ≤107cellsEnzymatic Reactions: varies
Isolation Technology:
Spin Column (Glass Fiber Filter), Organic Extraction
Elution Volume:
100 μL
Final Product Type:
Small RNA
For Use With (Application):
qRT-PCR, microarray analysis, Northern blotting
Shipping Condition:
Room Temperature
Yield:
Cells: ≤16 μg RNA per 50,000 cellsTissue: ≤160 μg per 5 mg
Content And Storage: DNA Hybridization Buffer, T7 Promoter Primer, Klenow Reaction Buffer, 10X dNTP, Exo– Klenow, T7 Enzyme Mix, T7 Reaction Buffer, 2X NTP Mix, Digestion Buffer, DNase, RNase, Sense Control DNA, and Antisense Control DNA should be stored at –20°C. siRNA Binding Buffer, siRNA Wash Buffer, Filter Cartridges, and Tubes should be stored at room temperature. Nuclease-free Water may be stored at any temperature.
Purification Time: __
Sample Type: In vitro Transcribed siRNA
High-throughput Compatibility: __
Quantity: __
Starting Material Amount: __
Isolation Technology: __
Elution Volume: __
Final Product Type: __
For Use With (Application): __
Shipping Condition: Dry Ice
Yield: __
Content And Storage:
DNA Hybridization Buffer, T7 Promoter Primer, Klenow Reaction Buffer, 10X dNTP, Exo– Klenow, T7 Enzyme Mix, T7 Reaction Buffer, 2X NTP Mix, Digestion Buffer, DNase, RNase, Sense Control DNA, and Antisense Control DNA should be stored at –20°C. siRNA Binding Buffer, siRNA Wash Buffer, Filter Cartridges, and Tubes should be stored at room temperature. Nuclease-free Water may be stored at any temperature.
Purification Time:
__
Sample Type:
In vitro Transcribed siRNA
High-throughput Compatibility:
__
Quantity:
__
Starting Material Amount:
__
Isolation Technology:
__
Elution Volume:
__
Final Product Type:
__
For Use With (Application):
__
Shipping Condition:
Dry Ice
Yield:
__
Content And Storage: DNA Hybridization Buffer, T7 Promoter Primer, Klenow Reaction Buffer, 10X dNTP, Exo– Klenow, T7 Enzyme Mix, T7 Reaction Buffer, 2X NTP Mix, Digestion Buffer, DNase, RNase, Sense Control DNA, and Antisense Control DNA should be stored at –20°C. siRNA Binding Buffer, siRNA Wash Buffer, Filter Cartridges, and Tubes should be stored at room temperature. Nuclease-free Water may be stored at any temperature.
Purification Time: __
Sample Type: In vitro Transcribed siRNA
High-throughput Compatibility: __
Quantity: __
Starting Material Amount: __
Isolation Technology: __
Elution Volume: __
Final Product Type: __
For Use With (Application): __
Shipping Condition: __
Yield: __
Content And Storage:
DNA Hybridization Buffer, T7 Promoter Primer, Klenow Reaction Buffer, 10X dNTP, Exo– Klenow, T7 Enzyme Mix, T7 Reaction Buffer, 2X NTP Mix, Digestion Buffer, DNase, RNase, Sense Control DNA, and Antisense Control DNA should be stored at –20°C. siRNA Binding Buffer, siRNA Wash Buffer, Filter Cartridges, and Tubes should be stored at room temperature. Nuclease-free Water may be stored at any temperature.
Purification Time:
__
Sample Type:
In vitro Transcribed siRNA
High-throughput Compatibility:
__
Quantity:
__
Starting Material Amount:
__
Isolation Technology:
__
Elution Volume:
__
Final Product Type:
__
For Use With (Application):
__
Shipping Condition:
__
Yield:
__
Content And Storage: T7 Enzyme Mix, 10X T7 Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, 10X Binding Buffer, Elution Solution, RNase, DNase I, 10X Digestion Buffer, 2X Wash Solution, and Control Template are all stored at –20°C, Nuclease-free Water may be store at any temperature. Filter Cartridges and Collection Tubes are stored at room temperature.
Purification Time: __
Sample Type: Double-Stranded RNA (Long)
High-throughput Compatibility: __
Quantity: __
Starting Material Amount: __
Isolation Technology: __
Elution Volume: __
Final Product Type: __
For Use With (Application): __
Shipping Condition: Wet Ice
Yield: __
Content And Storage:
T7 Enzyme Mix, 10X T7 Reaction Buffer, ATP Solution, CTP Solution, GTP Solution, UTP Solution, 10X Binding Buffer, Elution Solution, RNase, DNase I, 10X Digestion Buffer, 2X Wash Solution, and Control Template are all stored at –20°C, Nuclease-free Water may be store at any temperature. Filter Cartridges and Collection Tubes are stored at room temperature.
Purification Time:
__
Sample Type:
Double-Stranded RNA (Long)
High-throughput Compatibility:
__
Quantity:
__
Starting Material Amount:
__
Isolation Technology:
__
Elution Volume:
__
Final Product Type:
__
For Use With (Application):
__
Shipping Condition:
Wet Ice
Yield:
__