AM1839
Invitrogen™ MagMAX™-96 for Microarrays Total RNA Isolation Kit
Manufacturer: Invitrogen™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | AM1839-Each-of-1 | In Stock | ₹ 59,378.64 |
AM1839 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 59,378.64
GST (18%): ₹ 10,688.155
Total Price: ₹ 70,066.795
Content And Storage
Kit Contents (storage):• 1 Processing Plate & Lid (room temperature)• 12 mL Lysis/Binding Solution Concentrate (room temperature)• 18 mL Wash Solution 1 Concentrate (room temperature)• 72.5 mL Wash Solution 2 Concentrate (room temperature)• 14 mL Elution Buffer (room temperature)• 6 mL MagMAX™ TURBO™ DNase Buffer (4°C or room temperature)• 4 x 25 mL TRI Reagent™ (4°C)• 1.1 mL RNA Binding Beads (4°C)• 1.1 mL Lysis/Binding Enhancer (-20°C)• 215 μL TURBO™ DNase, 10 U/μL (-20°C)Contains enough reagents to isolate RNA from 96 samples.
Isolation Technology
Magnetic Bead, Organic Extraction
Sample Type
Cells, Mammalian Cells, Plant Samples, Tissue (Mammalian), Yeast
High-throughput Compatibility
High-throughput Compatible, Automated Protocols
For Use With (Application)
Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Product Line
Ambion™, MagMAX™
Target
Total RNA
Format
96-well Plate
Purification Time
<1 hr.
Final Product Type
Total RNA
For Use With (Equipment)
MagMAX™ Express 24 and 96, Robotic Liquid Handling Systems, MagMAX™ Express-96 Standard Magnetic Particle Processor
No. of Reactions
96 Preps
Starting Material Amount
Up to 5 x10^6 cells, Up to 100 mg tissue
Related Products
Description
- The MagMAX™-96 for Microarrays Total RNA Isolation Kit is for the rapid, high-throughput purification of RNA from mammalian cells and tissue in 96-well plates
- The kit combines an organic extraction with magnetic bead–based purification yielding extremely high-quality RNA suitable for the most stringent applications, such as microarray analysis
- The kit includes sufficient reagents for 96 reactions
- Advantages of the MagMAX™-96 for Microarrays Total RNA Isolation Kit: • Improve gene expression studies with consistent results from experiment to experiment • Combines TRI Reagent™ with a superior magnetic bead–based technology • Streamlined RNA purification, without sacrificing RNA quality or quantity • Requires less hands-on time than competitor kits • Can be integrated with established robotic platforms • Best MagMAX™ kit for difficult samples or large volumes Benefits of MagMAX™ magnetic bead–based purification Magnetic beads offer many benefits compared to other technologies for isolating RNA
- Beads bind RNA more efficiently than glass fiber filters, resulting in higher and more consistent RNA yields
- Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples
- Since only a small volume of magnetic beads is needed for high- efficiency binding, the bound RNA can be eluted in just 20–50 μL of nuclease-free water, concentrating RNA from large, dilute samples
- Highly stringent for the best-quality RNA The MagMAX™-96 for Microarrays Total RNA Isolation Kit (patent pending) procedure combines the robust and reliable lysis/denaturant TRI Reagent™ with MagMAX™ magnetic bead–based RNA purification technology, resulting in streamlined RNA purification, even in a high-throughput format, without sacrificing RNA quantity or quality
- TRI Reagent™ readily lyses even difficult cells and tissues while denaturing nucleases to maintain the integrity of RNA
- The kit provides high yields of pure, intact RNA that can be used directly for quantitative reverse transcriptase PCR (qRT-PCR) and microarray analysis
- See the accompanying data showing RNA yield and quality obtained when using the MagMAX™-96 for Microarrays Total RNA Isolation Kit
- Also shown is data illustrating a high correlation between the method that Affymetrix recommends for producing RNA for use on their GeneChips™ and the method that the MagMAX™-96 for Microarrays kit employs
- Fast, flexible procedure Two alternative protocols are supplied for the kit
- The “Spin” method has fewer steps, requires no DNase treatment, and allows for larger sample amounts to be processed
- The “No Spin” method requires less sample handling and can easily be integrated with a robotic liquid handler
- The procedure is fast (less than 1 hr), simple, and well suited for automation
- The kit can also be used to process fewer than 96 samples at one time and is compatible with either manual processing using multi-channel pipettors or processing with robotic liquid handlers
- Downloadable protocols for the use of this kit with specific liquid handling systems are available at our Automation Resource
- Accessory products A 96-well magnetic ring stand is required to use this kit
- The Magnetic Stand-96 (Cat
- No
- AM10027) and the 96-well Magnetic Ring Stand (Cat
- No
- AM10050) are designed for paramagnetic bead precipitation from standard 96-well, U-bottom microplates
- The Magnetic-Ring Stand pellets the magnetic beads in a circle shape, enabling easy removal of supernatant by a robot or multichannel pipettors
- For additional processing plates, Matrix 96-Well U Bottom Polypropylene Plates (Thermo Scientific, Item# 4917) are recommended.
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Content And Storage: Kit Contents (storage):• 1 Processing Plate & Lid (room temperature)• 12 mL Lysis/Binding Solution Concentrate (room temperature)• 18 mL Wash Solution 1 Concentrate (room temperature)• 72.5 mL Wash Solution 2 Concentrate (room temperature)• 14 mL Elution Buffer (room temperature)• 6 mL MagMAX™ TURBO™ DNase Buffer (4°C or room temperature)• 4 x 25 mL TRI Reagent™ (4°C)• 1.1 mL RNA Binding Beads (4°C)• 1.1 mL Lysis/Binding Enhancer (-20°C)• 215 μL TURBO™ DNase, 10 U/μL (-20°C)Contains enough reagents to isolate RNA from 96 samples.
Isolation Technology: Magnetic Bead, Organic Extraction
Sample Type: Cells, Mammalian Cells, Plant Samples, Tissue (Mammalian), Yeast
High-throughput Compatibility: High-throughput Compatible, Automated Protocols
For Use With (Application): Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Product Line: Ambion™, MagMAX™
Target: Total RNA
Format: 96-well Plate
Purification Time: <1 hr.
Final Product Type: Total RNA
For Use With (Equipment): MagMAX™ Express 24 and 96, Robotic Liquid Handling Systems, MagMAX™ Express-96 Standard Magnetic Particle Processor
No. of Reactions: 96 Preps
Starting Material Amount: Up to 5 x10^6 cells, Up to 100 mg tissue
Content And Storage:
Kit Contents (storage):• 1 Processing Plate & Lid (room temperature)• 12 mL Lysis/Binding Solution Concentrate (room temperature)• 18 mL Wash Solution 1 Concentrate (room temperature)• 72.5 mL Wash Solution 2 Concentrate (room temperature)• 14 mL Elution Buffer (room temperature)• 6 mL MagMAX™ TURBO™ DNase Buffer (4°C or room temperature)• 4 x 25 mL TRI Reagent™ (4°C)• 1.1 mL RNA Binding Beads (4°C)• 1.1 mL Lysis/Binding Enhancer (-20°C)• 215 μL TURBO™ DNase, 10 U/μL (-20°C)Contains enough reagents to isolate RNA from 96 samples.
Isolation Technology:
Magnetic Bead, Organic Extraction
Sample Type:
Cells, Mammalian Cells, Plant Samples, Tissue (Mammalian), Yeast
High-throughput Compatibility:
High-throughput Compatible, Automated Protocols
For Use With (Application):
Microarray Analysis, Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Product Line:
Ambion™, MagMAX™
Target:
Total RNA
Format:
96-well Plate
Purification Time:
<1 hr.
Final Product Type:
Total RNA
For Use With (Equipment):
MagMAX™ Express 24 and 96, Robotic Liquid Handling Systems, MagMAX™ Express-96 Standard Magnetic Particle Processor
No. of Reactions:
96 Preps
Starting Material Amount:
Up to 5 x10^6 cells, Up to 100 mg tissue
Content And Storage: • Carrier NA and Lysis/Binding Enhancer, store at -20°C• RNA Binding Beads, 4°C• Wash Solution 2 Concentrate, 4°C or room temperature• Elution Buffer, 4°C or room temperature• Lysis/Binding Solution Concentrate, room temperature• Wash Solution 1 Concentrate, room temperature• Processing Plates, room temperature• Lids, room temperature• PBS Buffer, room temperature
Isolation Technology: Magnetic Bead
Sample Type: Bacteria, Blood, Cells, Liquid Samples (e.g. Serum)
High-throughput Compatibility: High-throughput Compatible
For Use With (Application): Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Southern Blotting, PCR, Northern Blotting
Product Line: MagMAX™
Target: Total RNA, Total Nucleic Acids, Genomic DNA
Format: 96-well Plate
Purification Time: __
Final Product Type: RNA, DNA
For Use With (Equipment): __
No. of Reactions: __
Starting Material Amount: Up to 0.3 g solid samples or 175 μl liquid samples
Content And Storage:
• Carrier NA and Lysis/Binding Enhancer, store at -20°C• RNA Binding Beads, 4°C• Wash Solution 2 Concentrate, 4°C or room temperature• Elution Buffer, 4°C or room temperature• Lysis/Binding Solution Concentrate, room temperature• Wash Solution 1 Concentrate, room temperature• Processing Plates, room temperature• Lids, room temperature• PBS Buffer, room temperature
Isolation Technology:
Magnetic Bead
Sample Type:
Bacteria, Blood, Cells, Liquid Samples (e.g. Serum)
High-throughput Compatibility:
High-throughput Compatible
For Use With (Application):
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Southern Blotting, PCR, Northern Blotting
Product Line:
MagMAX™
Target:
Total RNA, Total Nucleic Acids, Genomic DNA
Format:
96-well Plate
Purification Time:
__
Final Product Type:
RNA, DNA
For Use With (Equipment):
__
No. of Reactions:
__
Starting Material Amount:
Up to 0.3 g solid samples or 175 μl liquid samples
Content And Storage: __
Isolation Technology: __
Sample Type: __
High-throughput Compatibility: __
For Use With (Application): __
Product Line: __
Target: __
Format: __
Purification Time: __
Final Product Type: __
For Use With (Equipment): __
No. of Reactions: __
Starting Material Amount: __
Content And Storage:
__
Isolation Technology:
__
Sample Type:
__
High-throughput Compatibility:
__
For Use With (Application):
__
Product Line:
__
Target:
__
Format:
__
Purification Time:
__
Final Product Type:
__
For Use With (Equipment):
__
No. of Reactions:
__
Starting Material Amount:
__
Content And Storage: __
Isolation Technology: __
Sample Type: __
High-throughput Compatibility: __
For Use With (Application): __
Product Line: __
Target: __
Format: __
Purification Time: __
Final Product Type: __
For Use With (Equipment): __
No. of Reactions: __
Starting Material Amount: __
Content And Storage:
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Isolation Technology:
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Sample Type:
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High-throughput Compatibility:
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For Use With (Application):
__
Product Line:
__
Target:
__
Format:
__
Purification Time:
__
Final Product Type:
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For Use With (Equipment):
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No. of Reactions:
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Starting Material Amount:
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