AM1830

Invitrogen™ MagMAX™-96 Total RNA Isolation Kit

Manufacturer: Invitrogen™

Select a Size

Pack Size SKU Availability Price
Each of 1 AM1830-Each-of-1 In Stock ₹ 42,437.76

AM1830 - Each of 1

₹ 42,437.76

In Stock

Quantity

1

Base Price: ₹ 42,437.76

GST (18%): ₹ 7,638.797

Total Price: ₹ 50,076.557

Content And Storage

• 1 Processing Plate; room temperature• 11 mL Lysis/Binding Solution (concentrated); room temperature• 18 mL Wash Solution 1 (concentrated); room temperature• 55 mL Wash Solution 2 (concentrated); room temperature• 12 mL RNA Rebinding (concentrated); room temperature• 10 mL Elution Buffer; room temperature• 6 mL MagMAX TURBO DNase Buffer; room temperature• 1.1 mL RNA Binding Beads; 4°C• 1.1 mL Lysis/Binding Enhancer; -20°C• 110 μL TURBO DNase; -20°C

Elution Volume

50 μL

Final Product Type

Total RNA

For Use With (Equipment)

KingFisher™ Duo Prime, KingFisher™ Flex, Automated Liquid Handling Systems

Quantity

96 Preps

Starting Material Amount

Cells: ≤106Plant: ≤10 mgTissue: ≤5 mg

Isolation Technology

Magnetic Bead

Sample Type

Cells, Plants, Tissue

High-throughput Compatibility

High-throughput Compatible

For Use With (Application)

RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling

Shipping Condition

Box 1: Dry IceBox 2: Room Temperature

Yield

≤20 pg per cell

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Description

  • The MagMAX-96 Total RNA Isolation Kit is designed for the rapid, moderate-throughput purification of RNA from animal and plant cells and tissue
  • RNA purified using the kit is ideal for PCR and RT-PCR applications
  • The kit uses magnetic bead–based purification and includes sufficient reagents for 96 reactions
  • Benefits of MagMAX magnetic bead–based purification Magnetic beads offer many benefits compared to other technologies for isolating RNA
  • Beads bind RNA more efficiently than glass fiber filters, resulting in higher and more consistent RNA yields
  • Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples
  • Since only a small volume of magnetic beads is needed for high-efficiency binding, the bound RNA can be eluted in just 20–50 μL of nuclease-free water, concentrating RNA from large, dilute samples
  • Accommodates diverse sample types The kit accommodates diverse biological samples and a broad range of cell and tissue input amounts
  • The kit is optimized for high-throughput isolation of RNA from 25 cells to 2 x 10 6 cells, as well as small plant and mammalian tissue samples (up to 10mg)
  • The protocol is fully amenable to automation
  • Detailed guidelines for general automation are included with the kit
  • Enhanced lysis/binding conditions to accommodate larger sample inputs Reagents for TURBO DNase™ treatment included to minimize DNA contamination and preserve RNA integrity Includes protocol for RNA isolation from plant tissues 96 reactions per kit suitable for moderate throughput Automated protocols, high-throughput compatible users Accessory products Lysis/Binding Solution Concentrate, Wash Solution 1 Concentrate, and Wash Solution 2 Concentrate are components of the kit that may also be purchased separately
  • The Plant RNA Isolation Aid can be used in conjunction with the MagMAX-96 Total RNA Isolation Kit for the isolation of RNA from plant tissues.

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Invitrogen™

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Content And Storage:
• 1 Processing Plate; room temperature• 11 mL Lysis/Binding Solution (concentrated); room temperature• 18 mL Wash Solution 1 (concentrated); room temperature• 55 mL Wash Solution 2 (concentrated); room temperature• 12 mL RNA Rebinding (concentrated); room temperature• 10 mL Elution Buffer; room temperature• 6 mL MagMAX TURBO DNase Buffer; room temperature• 1.1 mL RNA Binding Beads; 4°C• 1.1 mL Lysis/Binding Enhancer; -20°C• 110 μL TURBO DNase; -20°C

Elution Volume:
50 μL

Final Product Type:
Total RNA

For Use With (Equipment):
KingFisher™ Duo Prime, KingFisher™ Flex, Automated Liquid Handling Systems

Quantity:
96 Preps

Starting Material Amount:
Cells: ≤106Plant: ≤10 mgTissue: ≤5 mg

Isolation Technology:
Magnetic Bead

Sample Type:
Cells, Plants, Tissue

High-throughput Compatibility:
High-throughput Compatible

For Use With (Application):
RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling

Shipping Condition:
Box 1: Dry IceBox 2: Room Temperature

Yield:
≤20 pg per cell

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Content And Storage:
Carrier RNA is stored at –20°C; RNA Binding Beads are stored at 4°C. Wash Solution 2 Concentrate and Elution Buffer may be stored at either 4°C or room temperature. Lysis⁄Binding Solution Concentrate, Wash Solution 1 Concentrate, RNA Rebinding Concentrate, Bead Resuspension Solution, Processing Plates and Lids, and PBS Buffer are stored at room temperature. Nuclease-free water may be stored at any temperature.

Elution Volume:
__

Final Product Type:
Viral RNA

For Use With (Equipment):
KingFisher™ Flex Magnetic Particle Processor, KingFisher 96, MagMAX™ Express-96 Standard Magnetic Particle Processor

Quantity:
__

Starting Material Amount:
Up to 50μl of sample

Isolation Technology:
Magnetic Bead

Sample Type:
Liquid Samples (e.g. Serum), Viral Samples

High-throughput Compatibility:
High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols

For Use With (Application):
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting

Shipping Condition:
__

Yield:
5 ng

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Elution Volume:
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For Use With (Equipment):
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Starting Material Amount:
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Isolation Technology:
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High-throughput Compatibility:
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Content And Storage:
Carrier RNA and Lysis Binding Enhancer is stored at –20°C; RNA Binding Beads are stored at 4°C. Wash Solution 2 Concentrate and Elution Buffer may be stored at either 4°C or room temperature. Lysis⁄Binding Solution Concentrate, Wash Solution 1 Concentrate, and Processing Plates and Lids are stored at room temperature.

Elution Volume:
__

Final Product Type:
Viral DNA⁄RNA

For Use With (Equipment):
MagMAX™ Express-96 Magnetic Particle Processor, KingFisher 96, MagMAX™ Express-96 Standard Magnetic Particle Processor

Quantity:
__

Starting Material Amount:
Up to 50μl of sample

Isolation Technology:
Magnetic Bead

Sample Type:
Cell-free Biological Fluids, Liquid Samples (e.g. Serum), Viral Samples

High-throughput Compatibility:
High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols

For Use With (Application):
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting

Shipping Condition:
__

Yield:
5 ng