44-518-93
Invitrogen™ MagMAX™ for Stabilized Blood Tubes RNA Isolation Kit, compatible with Tempus™ Blood RNA Tubes
Manufacturer: Invitrogen™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 44-518-93-Each-of-1 | In Stock | ₹ 75,806.16 |
44-518-93 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 75,806.16
GST (18%): ₹ 13,645.109
Total Price: ₹ 89,451.269
Content And Storage
Box 1• 12 mL Tempus Resuspension Solution; room temperature• 5 mL Binding Solution (concentrated); room temperature• 2 x 16 mL Wash Solution 1 (concentrated); room temperature• 30 mL Wash Solution 2 (concentrated); room temperature• 10 mL Elution Buffer; room temperature• 1 Processing Plate; room temperature• 2 x 200 mL Tempus Pre-Digestion Wash; 4°C• 2 mL RNA Binding Beads; 4°C• 2 x 150 mL Tempus 1X PBS; 4°CBox 2• 0.25 mL Tempus Proteinase; -20°C• 1 mL TURBO DNase; -20°C
Purification Time
Less than 2 hr. for automated protocol
Sample Type
Whole Blood
High-throughput Compatibility
High-throughput Compatible
For Use With (Application)
RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling
Shipping Condition
Box 1: Room TemperatureBox 2: Cold Gel
Yield
≤25 μg
Isolation Technology
Magnetic Bead
Elution Volume
20 to 80 μL
Final Product Type
Total RNA
For Use With (Equipment)
KingFisher™ Duo Prime, KingFisher™ Flex
Quantity
96 preps
Starting Material Amount
3 mL of whole blood collected in Tempus™ Blood RNA Tube
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Description
- The MagMAX for Stabilized Blood Tubes RNA Isolation Kit purifies total RNA (including miRNA) from Tempus Blood RNA Tubes using proven magnetic bead-based technology
- A TURBO DNase™ step efficiently eliminates contaminating gDNA ensuring the highest purity RNA possible suitable for real-time RT-PCR and microarray analysis
- Maximize Total RNA Yields From Blood Samples The MagMAX for Stabilized Blood Tubes RNA Isolation Kit utilizes proven magnetic bead-based technology to purify maximal yields of total RNA from Tempus™ Blood RNA Tubes
- The MagMAX for Stabilized Blood Tubes RNA Isolation Kit can recover 3-25 μg from a single Tempus Blood RNA tube
- A TURBO DNase step efficiently eliminates contaminating gDNA that can interfere with downstream applications and ensures the highest purity RNA possible
- The MagMAX for Stabilized Blood Tubes RNA Isolation Kit can also detect miRNA from Tempus Blood RNA tubes as shown by TaqMan™ gene expression assays
- Users are strongly advised to perform appropriate validation studies against their miRNA targets
- Rapid Processing Time Processing times will vary based on format selected, but twelve Tempus Blood RNA tubes can be processed manually with the MagMAX for Stabilized Blood Tubes RNA Isolation Kit in approximately 2 hours
- An Alternative to Full Purification For users who perform primarily TaqMan-based real-time RT-PCR experiments, and do not need to archive RNA/cDNA, an alternative to the MagMAX for Stabilized Blood Tubes RNA Isolation Kit is the Stabilized Blood-to-CT™ Nucleic Acid Preparation Kit for qPCR
- This kit makes it possible to perform expression analysis directly from Tempus Blood RNA tubes without conventional RNA purification
- One-Stop Shopping MagMAX for Stabilized Blood Tubes RNA Isolation Kit is compatible with reverse transcription mixes (SuperScript™), PCR master mixes (both TaqMan and SYBRbased), and other blood workflow products
- Flexible purification format—use microfuge tubes, manual 96-well plates, or open-format automated 96-well plates from the same kit Simple magnetic bead workflow—no spin columns Total RNA without RNase activity, contaminating gDNA, or RT-PCR inhibitors Suitable for use with automated protocols, high-throughput compatible Flexible Purification Formats The MagMAX for Stabilized Blood Tubes RNA Isolation Kit can be processed in low-, medium- or high-throughput formats with superior workflow and handling compared to traditional competitive methods For low-throughput users, the MagMAX for Stabilized Blood Tubes RNA Isolation Kit can be used in individual 1.5 mL microfuge tubes with the bead-RNA-complex remaining in a single processing tube until the final elution step For medium-to-high throughput users, the MagMAX for Stabilized Blood Tubes RNA Isolation Kit can be done in a 96-well processing plate with either a single or multi-channel pipettor to accelerate the purification process For high-throughput users, the MagMAX for Stabilized Blood Tubes RNA Isolation Kit is compatible with manual and automated 96-well processing Simply process multiple batches of tubes in series or in parallel, through the up-front centrifugation process, to the convenient stopping point, until enough samples can be loaded onto a 96-well processing plate for the RNA purification steps The RNA purification process can be handled on either open-format liquid handlers, in conjunction with a 96-well magnetic stand on the deck, or magnetic bead movers like the MagMAX Express-96 Magnetic Particle Processor (deep well version only)
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Content And Storage: Box 1• 12 mL Tempus Resuspension Solution; room temperature• 5 mL Binding Solution (concentrated); room temperature• 2 x 16 mL Wash Solution 1 (concentrated); room temperature• 30 mL Wash Solution 2 (concentrated); room temperature• 10 mL Elution Buffer; room temperature• 1 Processing Plate; room temperature• 2 x 200 mL Tempus Pre-Digestion Wash; 4°C• 2 mL RNA Binding Beads; 4°C• 2 x 150 mL Tempus 1X PBS; 4°CBox 2• 0.25 mL Tempus Proteinase; -20°C• 1 mL TURBO DNase; -20°C
Purification Time: Less than 2 hr. for automated protocol
Sample Type: Whole Blood
High-throughput Compatibility: High-throughput Compatible
For Use With (Application): RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling
Shipping Condition: Box 1: Room TemperatureBox 2: Cold Gel
Yield: ≤25 μg
Isolation Technology: Magnetic Bead
Elution Volume: 20 to 80 μL
Final Product Type: Total RNA
For Use With (Equipment): KingFisher™ Duo Prime, KingFisher™ Flex
Quantity: 96 preps
Starting Material Amount: 3 mL of whole blood collected in Tempus™ Blood RNA Tube
Content And Storage:
Box 1• 12 mL Tempus Resuspension Solution; room temperature• 5 mL Binding Solution (concentrated); room temperature• 2 x 16 mL Wash Solution 1 (concentrated); room temperature• 30 mL Wash Solution 2 (concentrated); room temperature• 10 mL Elution Buffer; room temperature• 1 Processing Plate; room temperature• 2 x 200 mL Tempus Pre-Digestion Wash; 4°C• 2 mL RNA Binding Beads; 4°C• 2 x 150 mL Tempus 1X PBS; 4°CBox 2• 0.25 mL Tempus Proteinase; -20°C• 1 mL TURBO DNase; -20°C
Purification Time:
Less than 2 hr. for automated protocol
Sample Type:
Whole Blood
High-throughput Compatibility:
High-throughput Compatible
For Use With (Application):
RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling
Shipping Condition:
Box 1: Room TemperatureBox 2: Cold Gel
Yield:
≤25 μg
Isolation Technology:
Magnetic Bead
Elution Volume:
20 to 80 μL
Final Product Type:
Total RNA
For Use With (Equipment):
KingFisher™ Duo Prime, KingFisher™ Flex
Quantity:
96 preps
Starting Material Amount:
3 mL of whole blood collected in Tempus™ Blood RNA Tube
Content And Storage: Box 1• 2 mL RNA Binding Beads; 4°C• 18 mL Resuspension Solution; room temperature• 16 mL Wash Solution 1 (concentrated); room temperature• 2 x 30 mL Wash Solution 2 (concentrated); room temperature• 10 mL RNA Rebinding Buffer; room temperature• 10 mL Elution Buffer; room temperature• 1 Processing Plate; room temperature• 100 Tubes; room temperatureBox 2• 0.6 mL Proteinase; -20°C• 1 mL TURBO DNase; -20°C• 1 mL 10X TURBO DNase Buffer; -20°C
Purification Time: Less than 2 hr. when using automated protocol
Sample Type: Whole Blood
High-throughput Compatibility: High-throughput Compatible
For Use With (Application): RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling
Shipping Condition: Box 1: Room TemperatureBox 2: Cold Gel
Yield: ≤18 μg
Isolation Technology: Magnetic Bead
Elution Volume: 20 to 80 μL
Final Product Type: Total RNA
For Use With (Equipment): __
Quantity: 96 preps
Starting Material Amount: 2.5 mL of blood in PAXgene™ tube
Content And Storage:
Box 1• 2 mL RNA Binding Beads; 4°C• 18 mL Resuspension Solution; room temperature• 16 mL Wash Solution 1 (concentrated); room temperature• 2 x 30 mL Wash Solution 2 (concentrated); room temperature• 10 mL RNA Rebinding Buffer; room temperature• 10 mL Elution Buffer; room temperature• 1 Processing Plate; room temperature• 100 Tubes; room temperatureBox 2• 0.6 mL Proteinase; -20°C• 1 mL TURBO DNase; -20°C• 1 mL 10X TURBO DNase Buffer; -20°C
Purification Time:
Less than 2 hr. when using automated protocol
Sample Type:
Whole Blood
High-throughput Compatibility:
High-throughput Compatible
For Use With (Application):
RT-PCR, qPCR, cDNA library construction, NGS, microarray analysis, blot hybridization, Northern blotting, in vitro translation, nuclease protection assays, nucleic acid labeling
Shipping Condition:
Box 1: Room TemperatureBox 2: Cold Gel
Yield:
≤18 μg
Isolation Technology:
Magnetic Bead
Elution Volume:
20 to 80 μL
Final Product Type:
Total RNA
For Use With (Equipment):
__
Quantity:
96 preps
Starting Material Amount:
2.5 mL of blood in PAXgene™ tube
Content And Storage: • PrepSEQ Proteinase K (store at -15°C to -25°C)• RNASE Cocktail (-15°C to -25°C)• PrepSEQ Extraction Kit including lysis buffer, Proteinase K buffer, binding solution, wash and elution buffers (room temperature)• PrepSEQ Magnetic Beads (room temperature)
Purification Time: __
Sample Type: Cell Culture Supernatants
High-throughput Compatibility: __
For Use With (Application): Cell Culture Contamination Detection
Shipping Condition: __
Yield: __
Isolation Technology: Magnetic Bead
Elution Volume: __
Final Product Type: Genomic DNA, Viral DNA, Bacterial DNA
For Use With (Equipment): __
Quantity: 100 Preps
Starting Material Amount: __
Content And Storage:
• PrepSEQ Proteinase K (store at -15°C to -25°C)• RNASE Cocktail (-15°C to -25°C)• PrepSEQ Extraction Kit including lysis buffer, Proteinase K buffer, binding solution, wash and elution buffers (room temperature)• PrepSEQ Magnetic Beads (room temperature)
Purification Time:
__
Sample Type:
Cell Culture Supernatants
High-throughput Compatibility:
__
For Use With (Application):
Cell Culture Contamination Detection
Shipping Condition:
__
Yield:
__
Isolation Technology:
Magnetic Bead
Elution Volume:
__
Final Product Type:
Genomic DNA, Viral DNA, Bacterial DNA
For Use With (Equipment):
__
Quantity:
100 Preps
Starting Material Amount:
__
Content And Storage: Store at room temperature.
Purification Time: __
Sample Type: __
High-throughput Compatibility: __
For Use With (Application): __
Shipping Condition: __
Yield: __
Isolation Technology: __
Elution Volume: __
Final Product Type: __
For Use With (Equipment): Veriti Dx Thermal Cycler
Quantity: 1 instrument
Starting Material Amount: __
Content And Storage:
Store at room temperature.
Purification Time:
__
Sample Type:
__
High-throughput Compatibility:
__
For Use With (Application):
__
Shipping Condition:
__
Yield:
__
Isolation Technology:
__
Elution Volume:
__
Final Product Type:
__
For Use With (Equipment):
Veriti Dx Thermal Cycler
Quantity:
1 instrument
Starting Material Amount:
__