Invitrogen™ Tempus™ Spin RNA Isolation Kit

Description

• This Applied Biosystems product is for the fast, easy, glass fiber filter-based isolation of RNA from blood using standard laboratory equipment in about one hour
• Contains sufficient reagents for RNA purification from 50 samples
• Gene expression profiling and analysis of whole blood is becoming an increasingly important life science tool
• Accurately measuring the molecular profile of blood-based RNA transcripts is a critical need in areas of research such as disease association studies, biomarker discovery and validation, and monitoring of drug treatment regimes
• Isolate high-quality RNA from blood, suitable for real-time PCR and microarray analysis Obtain highly stable RNA virtually free of RNase activity, contaminating gDNA, or RT-PCR inhibitors Easy workflow requires only standard laboratory centrifuges and ∼1 hour of time No separate RBC lysis or proteinase K treatment required Performance with Ease The ability of the Tempus system (Tempus Blood RNA Tubes and Tempus Spin RNA Isolation Kit) to stabilize RNA expression profiles has been tested over a range of storage conditions
• The combination provides stable RNA, high yields of purified RNA (observed yields of 6 to 25μg from a full blood draw from normal, healthy donors), and high-quality RNA (A260/280 ratio greater than 1.9 and RIN greater than 7.0) suitable for use in downstream applications such as quantitative PCR, microarray profiling, and other RNA analysis methods
• Accessory Product The highly unstable nature of RNA transcripts ex vivo creates an even greater challenge for accurate expression analysis
• Ongoing metabolic changes can drastically affect gene expression profiles in as little as one hour in standard blood collection tubes
• Use of the Applied Biosystems Tempus™ Blood RNA Tubes (Cat
• No
• 4342792) is therefore recommended
• These tubes are designed for direct collection of 3mL of blood into a plastic, evacuated blood collection tube containing RNA Stabilizing Reagent
• The RNA, and therefore the expression profile, is stable in the collection tube for up to five days at room temperature and a minimum of 7 days at 4°C Not high-throughput compatible (Manual)