AM1835
Applied Biosystems™ MagMAX™-96 AI/ND Viral RNA Isolation Kit
Manufacturer: Applied Biosystems™
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | AM1835-Each-of-1 | In Stock | ₹ 1,68,553.20 |
AM1835 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,68,553.20
GST (18%): ₹ 30,339.576
Total Price: ₹ 1,98,892.776
Content And Storage
Carrier RNA is stored at –20°C; RNA Binding Beads are stored at 4°C. Wash Solution 2 Concentrate and Elution Buffer may be stored at either 4°C or room temperature. Lysis⁄Binding Solution Concentrate, Wash Solution 1 Concentrate, RNA Rebinding Concentrate, Bead Resuspension Solution, Processing Plates and Lids, and PBS Buffer are stored at room temperature. Nuclease-free water may be stored at any temperature.
Isolation Technology
Magnetic Bead
Sample Type
Liquid Samples (e.g. Serum), Viral Samples
High-throughput Compatibility
High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols
For Use With (Application)
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Product Line
MagMAX™
Target
Viral DNA/RNA
Format
96-well Plate
Purification Time
1 hr.
Final Product Type
Viral RNA
For Use With (Equipment)
KingFisher™ Flex Magnetic Particle Processor, KingFisher 96, MagMAX™ Express-96 Standard Magnetic Particle Processor
No. of Reactions
384 preps
Starting Material Amount
Up to 50μl of sample
Yield
5 ng
Related Products
Description
- The MagMAX™-96 AI/ND Viral RNA Isolation Kit is a first generation MagMAX™ Viral RNA Isolation Kit validated by the NVSL for use with AI/ND viruses
- Perfect for working with low viral concentrations, the kit contains sufficient reagents for 4 x 96 reactions
- Validated by the NVSL for use with AI/ND viruses Recover as few as 10 copies of viral RNA from 100 to 400 μL sample Typical viral RNA recovery of >50% Ideal when working with low viral concentrations Linear recovery down to 50 transcripts 96 samples can be processed in 30 minutes Easily integrated into robotic platforms Benefits of MagMAX™ Magnetic Bead-Based Purification: Magnetic beads offer many benefits compared to other technologies for isolating RNA
- Beads bind RNA more efficiently than glass fiber filters, resulting in higher and more consistent RNA yields
- Additionally, because filters are not used, there is no risk of filter clogging due to cellular particulates in samples
- Since only a small volume of magnetic beads is needed for high-efficiency binding, the bound RNA can be eluted in just 20 to 50 μL of nuclease-free water, concentrating RNA from large, dilute samples
- Optimized for Isolation of Virus from Biofluids: The Applied Biosystems™ MagMAX™ Viral RNA Isolation Kit has been optimized for use with biological fluids and cell-free samples such as serum, plasma, swabs, and cell culture media
- These samples often present unique challenges when purifying RNA, including low viral titers, large volumes to be processed, and sample complexity
- Without the need for organic solvents or RNA precipitation, the MagMAX™ Viral RNA isolation kit eliminates the problems often encountered with other RNA isolation methods such filter clogging, large elution volumes, and inconsistent yields
- Get the Same Amount of RNA from 10-fold Less Sample The sensitivity of viral RNA detection was compared using RNA isolated with Applied Biosystems™ MagMAX™ protocol or the current National Veterinary Services Laboratories (NVSL) validated viral testing protocol
- Even at one-tenth the sample input, viral RNA isolated using the MagMAX™ kit could be detected at equal or greater levels than RNA isolated using the NVSL protocol and recommended competitor's kit
- Accessory Products: Carrier RNA (SKU#4382878), Lysis/Binding Solution Concentrate (SKU#AM8500), Wash Solution 1 Concentrate (SKU#AM8504), and Wash Solution 2 Concentrate (SKU#AM8640) are components of the MagMAX™ Viral RNA Isolation Kits that may also be purchased separately
- A 96-well magnetic ring stand is required to use this kit
- The Magnetic Stand-96 (SKU#AM10027) and the 96-well Magnetic Ring Stand (SKU#AM10050) are designed for paramagnetic bead precipitation from standard 96 well U-bottom microplates
- The Magnetic-Ring Stand pellets the magnetic beads in a circle shape, enabling easy removal of supernatant by a robot or multichannel pipettors
- For additional processing plates, Matrix 96-Well U Bottom Polypropylene Plates (Thermo Scientific, Item# 4917) are recommended
- The MagMAX™ and MagMAX™-96 Viral RNA Isolation Kits (SKU#AM1939 and SKU#AM1836) are the newest generation MagMAX™ kits for this application, but are not yet validated by the NVSL.
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Content And Storage: Carrier RNA is stored at –20°C; RNA Binding Beads are stored at 4°C. Wash Solution 2 Concentrate and Elution Buffer may be stored at either 4°C or room temperature. Lysis⁄Binding Solution Concentrate, Wash Solution 1 Concentrate, RNA Rebinding Concentrate, Bead Resuspension Solution, Processing Plates and Lids, and PBS Buffer are stored at room temperature. Nuclease-free water may be stored at any temperature.
Isolation Technology: Magnetic Bead
Sample Type: Liquid Samples (e.g. Serum), Viral Samples
High-throughput Compatibility: High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols
For Use With (Application): Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Product Line: MagMAX™
Target: Viral DNA/RNA
Format: 96-well Plate
Purification Time: 1 hr.
Final Product Type: Viral RNA
For Use With (Equipment): KingFisher™ Flex Magnetic Particle Processor, KingFisher 96, MagMAX™ Express-96 Standard Magnetic Particle Processor
No. of Reactions: 384 preps
Starting Material Amount: Up to 50μl of sample
Yield: 5 ng
Content And Storage:
Carrier RNA is stored at –20°C; RNA Binding Beads are stored at 4°C. Wash Solution 2 Concentrate and Elution Buffer may be stored at either 4°C or room temperature. Lysis⁄Binding Solution Concentrate, Wash Solution 1 Concentrate, RNA Rebinding Concentrate, Bead Resuspension Solution, Processing Plates and Lids, and PBS Buffer are stored at room temperature. Nuclease-free water may be stored at any temperature.
Isolation Technology:
Magnetic Bead
Sample Type:
Liquid Samples (e.g. Serum), Viral Samples
High-throughput Compatibility:
High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols
For Use With (Application):
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Product Line:
MagMAX™
Target:
Viral DNA/RNA
Format:
96-well Plate
Purification Time:
1 hr.
Final Product Type:
Viral RNA
For Use With (Equipment):
KingFisher™ Flex Magnetic Particle Processor, KingFisher 96, MagMAX™ Express-96 Standard Magnetic Particle Processor
No. of Reactions:
384 preps
Starting Material Amount:
Up to 50μl of sample
Yield:
5 ng
Content And Storage: __
Isolation Technology: __
Sample Type: __
High-throughput Compatibility: __
For Use With (Application): __
Product Line: __
Target: __
Format: __
Purification Time: __
Final Product Type: __
For Use With (Equipment): __
No. of Reactions: __
Starting Material Amount: __
Yield: __
Content And Storage:
__
Isolation Technology:
__
Sample Type:
__
High-throughput Compatibility:
__
For Use With (Application):
__
Product Line:
__
Target:
__
Format:
__
Purification Time:
__
Final Product Type:
__
For Use With (Equipment):
__
No. of Reactions:
__
Starting Material Amount:
__
Yield:
__
Content And Storage: Carrier RNA and Lysis Binding Enhancer is stored at –20°C; RNA Binding Beads are stored at 4°C. Wash Solution 2 Concentrate and Elution Buffer may be stored at either 4°C or room temperature. Lysis⁄Binding Solution Concentrate, Wash Solution 1 Concentrate, and Processing Plates and Lids are stored at room temperature.
Isolation Technology: Magnetic Bead
Sample Type: Cell-free Biological Fluids, Liquid Samples (e.g. Serum), Viral Samples
High-throughput Compatibility: High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols
For Use With (Application): Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Product Line: MagMAX™
Target: Viral DNA/RNA
Format: 96-well Plate
Purification Time: 45 min.
Final Product Type: Viral DNA⁄RNA
For Use With (Equipment): MagMAX™ Express-96 Magnetic Particle Processor, KingFisher 96, MagMAX™ Express-96 Standard Magnetic Particle Processor
No. of Reactions: 96
Starting Material Amount: Up to 50μl of sample
Yield: 5 ng
Content And Storage:
Carrier RNA and Lysis Binding Enhancer is stored at –20°C; RNA Binding Beads are stored at 4°C. Wash Solution 2 Concentrate and Elution Buffer may be stored at either 4°C or room temperature. Lysis⁄Binding Solution Concentrate, Wash Solution 1 Concentrate, and Processing Plates and Lids are stored at room temperature.
Isolation Technology:
Magnetic Bead
Sample Type:
Cell-free Biological Fluids, Liquid Samples (e.g. Serum), Viral Samples
High-throughput Compatibility:
High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols
For Use With (Application):
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Product Line:
MagMAX™
Target:
Viral DNA/RNA
Format:
96-well Plate
Purification Time:
45 min.
Final Product Type:
Viral DNA⁄RNA
For Use With (Equipment):
MagMAX™ Express-96 Magnetic Particle Processor, KingFisher 96, MagMAX™ Express-96 Standard Magnetic Particle Processor
No. of Reactions:
96
Starting Material Amount:
Up to 50μl of sample
Yield:
5 ng
Content And Storage: Store Processing Plate with Lid, Lysis/Binding Solution Concentrate, Wash Solution 1 Concentrate, Wash Solution 2 Concentrate, and Elution Buffer at room temp.Store MagMax Turbo DNase Buffer and RNA Binding Beads at 4°C.Store Lysis/Binding Enhancer and TURBO DNase at -20°C.
Isolation Technology: Magnetic Bead
Sample Type: Liquid Samples (e.g. Serum), Milk, Viral Samples, Whole Blood (Mammalian)
High-throughput Compatibility: High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols
For Use With (Application): Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Product Line: Ambion™, MagMAX™
Target: Viral DNA/RNA, Total RNA
Format: 96-well Plate
Purification Time: __
Final Product Type: Total RNA, Viral RNA
For Use With (Equipment): MagMAX™ Express 24 and 96, Robotic Liquid Handling Systems, MagMAX™ Express-96 Standard Magnetic Particle Processor
No. of Reactions: 96 Preps
Starting Material Amount: Up to 50 μl whole blood
Yield: 0.5 μg
Content And Storage:
Store Processing Plate with Lid, Lysis/Binding Solution Concentrate, Wash Solution 1 Concentrate, Wash Solution 2 Concentrate, and Elution Buffer at room temp.Store MagMax Turbo DNase Buffer and RNA Binding Beads at 4°C.Store Lysis/Binding Enhancer and TURBO DNase at -20°C.
Isolation Technology:
Magnetic Bead
Sample Type:
Liquid Samples (e.g. Serum), Milk, Viral Samples, Whole Blood (Mammalian)
High-throughput Compatibility:
High-throughput Compatible, Not High-throughput Compatible (Manual), Automated Protocols
For Use With (Application):
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR), Northern Blotting
Product Line:
Ambion™, MagMAX™
Target:
Viral DNA/RNA, Total RNA
Format:
96-well Plate
Purification Time:
__
Final Product Type:
Total RNA, Viral RNA
For Use With (Equipment):
MagMAX™ Express 24 and 96, Robotic Liquid Handling Systems, MagMAX™ Express-96 Standard Magnetic Particle Processor
No. of Reactions:
96 Preps
Starting Material Amount:
Up to 50 μl whole blood
Yield:
0.5 μg