AM1901
Invitrogen™ MICROBEnrich™ Kit
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | AM1901-Each-of-1 | In Stock | ₹ 64,436.00 |
AM1901 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 64,436.00
GST (18%): ₹ 11,598.48
Total Price: ₹ 76,034.48
Content And Storage
Store Control RNA, Capture Oligo Mix, Glycogen, and 3M NaOAc at -20°C.Store Oligo MagBeads, Binding Buffer, Wash Solution, Regeneration Solution 1, Regeneration Solution 2, and Resuspension Solution at 4°C.Store Nuclease-free Water, 1.5 mL tubes and 2 mL collection tubes at room temperature.
Sample Type
Bacteria, RNA, Total RNA (Mammalian Host-Bacterial RNA Mixture)
High-throughput Compatibility
Not High-throughput Compatible (Manual)
No. of Reactions
20 Preps
Target
Total RNA
Isolation Technology
Hybridization Capture, Magnetic Bead
Final Product Type
Total RNA (Bacterial)
For Use With (Application)
Microarray Analysis
Product Line
Ambion™, MICROBEnrich™
Related Products
Description
- For the separation and isolation of bacterial RNA from mixed samples
- The Ambion™ kit includes sufficient reagents for depleting 500 μg of host cell RNA (20 reactions x 25 μg RNA) from a complex host-bacterial RNA mixture
- Gene expression analysis following host-bacterial interactions is revolutionizing our understanding of the host response to bacterial infection
- Numerous studies have utilized microarray analysis to follow host cell transcriptome alterations in response to interactions with bacteria
- However, because no method has existed to isolate bacterial RNA away from host cell RNA, the corresponding analyses of bacterial transcriptomes has been impossible
- The MICROB Enrich ™ Kit enables whole genome microarray expression analyses of bacterial RNA after host-bacterial interactions
- Start With Any Total RNA Sample For microarray analysis and other sensitive applications, a DNase treatment is recommended to remove contaminating genomic DNA from the total RNA prior to the MICROB Enrich™ Kit procedure
- Remove >90% of Mammalian RNA from Complex Total RNA Mixtures The MICROB Enrich ™ Kituses hybridization capture technology (patent pending) to remove human, mouse, and rat RNA (both mRNA and rRNA) from complex host-bacterial RNA populations, leaving behind enriched microbial total RNA
- In the first step of the procedure, host-bacterial total RNA is incubated with a mixture of capture oligonucleotides that bind the mammalian 18S and 28S rRNAs and polyadenylated RNAs
- Next, the rRNA/oligo nucleotide hybrids and all polyadenylated mRNAs are removed from the mixture with oligonucleotide-derivatized magnetic beads
- After magnetic separation, the bacterial RNA remaining in the supernatant and can be precipitated with ethanol
- An Agilent 2100 bioanalyzer electropherogram (see Figure) shows nearly complete removal (>95%) of the host 18S and 28S rRNAs from a mixed sample
- Bacterial mRNAs purified with the MICROB Enrich™ Kit are better templates than total RNA for downstream applications and therefore deliver dramatic increases in sensitivity when used in applications such as array analysis (see Figure)
- The MICROB Enrich™ Kit Will Work With Any Bacterial Species The MICROB Enrich ™ Kit was optimized using E
- coli RNA in a background of human, mouse, and rat RNA
- However, the kit should perform well with RNA from any bacterial species
- Although many mammalian host species may be compatible with the MICROB Enrich ™ Kit, only human, mouse, and rat rRNA sequences were used for the design and optimization of the capture oligonucleotides.
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Content And Storage: Store Control RNA, Capture Oligo Mix, Glycogen, and 3M NaOAc at -20°C.Store Oligo MagBeads, Binding Buffer, Wash Solution, Regeneration Solution 1, Regeneration Solution 2, and Resuspension Solution at 4°C.Store Nuclease-free Water, 1.5 mL tubes and 2 mL collection tubes at room temperature.
Sample Type: Bacteria, RNA, Total RNA (Mammalian Host-Bacterial RNA Mixture)
High-throughput Compatibility: Not High-throughput Compatible (Manual)
No. of Reactions: 20 Preps
Target: Total RNA
Isolation Technology: Hybridization Capture, Magnetic Bead
Final Product Type: Total RNA (Bacterial)
For Use With (Application): Microarray Analysis
Product Line: Ambion™, MICROBEnrich™
Content And Storage:
Store Control RNA, Capture Oligo Mix, Glycogen, and 3M NaOAc at -20°C.Store Oligo MagBeads, Binding Buffer, Wash Solution, Regeneration Solution 1, Regeneration Solution 2, and Resuspension Solution at 4°C.Store Nuclease-free Water, 1.5 mL tubes and 2 mL collection tubes at room temperature.
Sample Type:
Bacteria, RNA, Total RNA (Mammalian Host-Bacterial RNA Mixture)
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
No. of Reactions:
20 Preps
Target:
Total RNA
Isolation Technology:
Hybridization Capture, Magnetic Bead
Final Product Type:
Total RNA (Bacterial)
For Use With (Application):
Microarray Analysis
Product Line:
Ambion™, MICROBEnrich™
Content And Storage: __
Sample Type: __
High-throughput Compatibility: __
No. of Reactions: __
Target: __
Isolation Technology: __
Final Product Type: __
For Use With (Application): __
Product Line: __
Content And Storage:
__
Sample Type:
__
High-throughput Compatibility:
__
No. of Reactions:
__
Target:
__
Isolation Technology:
__
Final Product Type:
__
For Use With (Application):
__
Product Line:
__
Content And Storage: Store Control RNA, Capture Oligo Mix, Glycogen, and 3M Sodium Acetate at -20°C.Store Oligo MagBeads, Binding Buffer, and Wash Solution at 4°CStore Nuclease-free Water, 1.5mL tubes, and Collection tubes at room temperature.
Sample Type: Total RNA (Bacterial)
High-throughput Compatibility: Not High-throughput Compatible (Manual)
No. of Reactions: 20 Preps
Target: Total RNA
Isolation Technology: Hybridization Capture, Magnetic Bead
Final Product Type: mRNA (Bacterial)
For Use With (Application): Microarray Analysis, Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction, Northern Blotting
Product Line: Ambion™, MICROBEnrich™
Content And Storage:
Store Control RNA, Capture Oligo Mix, Glycogen, and 3M Sodium Acetate at -20°C.Store Oligo MagBeads, Binding Buffer, and Wash Solution at 4°CStore Nuclease-free Water, 1.5mL tubes, and Collection tubes at room temperature.
Sample Type:
Total RNA (Bacterial)
High-throughput Compatibility:
Not High-throughput Compatible (Manual)
No. of Reactions:
20 Preps
Target:
Total RNA
Isolation Technology:
Hybridization Capture, Magnetic Bead
Final Product Type:
mRNA (Bacterial)
For Use With (Application):
Microarray Analysis, Reverse Transcriptase PCR (RT-PCR), cDNA Library Construction, Northern Blotting
Product Line:
Ambion™, MICROBEnrich™
Content And Storage: Store rDNase1 , 10X DNase 1 Buffer and DNase Inactivation Reagent at -20°C. Store Nuclease–free Water at room temperature.
Sample Type: __
High-throughput Compatibility: __
No. of Reactions: __
Target: __
Isolation Technology: __
Final Product Type: __
For Use With (Application): __
Product Line: Ambion™, DNA-free™
Content And Storage:
Store rDNase1 , 10X DNase 1 Buffer and DNase Inactivation Reagent at -20°C. Store Nuclease–free Water at room temperature.
Sample Type:
__
High-throughput Compatibility:
__
No. of Reactions:
__
Target:
__
Isolation Technology:
__
Final Product Type:
__
For Use With (Application):
__
Product Line:
Ambion™, DNA-free™