12-339-024
Invitrogen™ AccuPrime™TaqDNA Polymerase System
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 12-339-024-Each-of-1 | In Stock | ₹ 1,70,264.40 |
12-339-024 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,70,264.40
GST (18%): ₹ 30,647.592
Total Price: ₹ 2,00,911.992
Content And Storage
• AccuPrime Taq DNA Polymerase (500 μL, at 2 U/μL)• 10X AccuPrime PCR Buffer I (2 x 1.25 mL)• 10X AccuPrime PCR Buffer II (2 x 1.25 mL)• 50 mM Magnesium Chloride (2 x 1.25 mL)Store at -20°C.
GC-Rich PCR Performance
Low
Polymerase
AccuPrime Taq DNA Polymerase
Product Type
DNA Polymerase System
For Use With (Application)
Hot-start PCR
Fidelity (vs. Taq)
2X
No. of Reactions
1000 Reactions
Reaction Format
Separate Components
Detection Method
Primer-probe
PCR Method
1-step RT-qPCR, Multiplex qPCR
Reaction Speed
Standard
Shipping Condition
Wet or Dry Ice
Concentration
10X
Hot Start
Built-In Hot Start
Overhang
3'-A
Size (Final Product)
5 kb or less
Related Products
Description
- The AccuPrime Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with an antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases
- Platinum anti- Taq DNA polymerase antibodies inhibit polymerase activity, providing an automatic hot-start, while a thermostable accessory protein enhances specific primer-template hybridization during every cycle of PCR
- This combination improves the fidelity of Taq DNA Polymerase by two-fold and is ideal for high-throughput screening and multiplex PCR
- AccuPrime Taq DNA Polymerase broadens primer annealing temperatures, giving you optimal performance between 55°C and 65°C
- AccuPrime Taq DNA Polymerase features Control mispriming at every cycle for single-band PCR products Minimal reaction optimization and primer set redesign Reaction assembly at room temperature Applications AccuPrime Taq DNA Polymerase can be used for a wide variety of applications, including: Multiplex PCR TOPO TA Cloning Allele-specific amplifications High-throughput screening AccuPrime buffers The 10X AccuPrime buffers contain thermostable AccuPrime protein, Mg ++ , and deoxyribonucleotide triphosphates at concentrations sufficient to allow amplification during PCR
- Two individual buffer systems (10X AccuPrime PCR Buffer I and II) are provided for amplification of specific types of templates
- 10X AccuPrime PCR Buffer I is designed for small genomic DNA amplicons (≤200 bp), cDNA, or plasmids
- 10X AccuPrime PCR Buffer II is designed for genomic DNA (200 bp–4 kb)
- Order Info Shipping Condition: Approved for shipment on wet or dry ice
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Content And Storage: • AccuPrime Taq DNA Polymerase (500 μL, at 2 U/μL)• 10X AccuPrime PCR Buffer I (2 x 1.25 mL)• 10X AccuPrime PCR Buffer II (2 x 1.25 mL)• 50 mM Magnesium Chloride (2 x 1.25 mL)Store at -20°C.
GC-Rich PCR Performance: Low
Polymerase: AccuPrime Taq DNA Polymerase
Product Type: DNA Polymerase System
For Use With (Application): Hot-start PCR
Fidelity (vs. Taq): 2X
No. of Reactions: 1000 Reactions
Reaction Format: Separate Components
Detection Method: Primer-probe
PCR Method: 1-step RT-qPCR, Multiplex qPCR
Reaction Speed: Standard
Shipping Condition: Wet or Dry Ice
Concentration: 10X
Hot Start: Built-In Hot Start
Overhang: 3'-A
Size (Final Product): 5 kb or less
Content And Storage:
• AccuPrime Taq DNA Polymerase (500 μL, at 2 U/μL)• 10X AccuPrime PCR Buffer I (2 x 1.25 mL)• 10X AccuPrime PCR Buffer II (2 x 1.25 mL)• 50 mM Magnesium Chloride (2 x 1.25 mL)Store at -20°C.
GC-Rich PCR Performance:
Low
Polymerase:
AccuPrime Taq DNA Polymerase
Product Type:
DNA Polymerase System
For Use With (Application):
Hot-start PCR
Fidelity (vs. Taq):
2X
No. of Reactions:
1000 Reactions
Reaction Format:
Separate Components
Detection Method:
Primer-probe
PCR Method:
1-step RT-qPCR, Multiplex qPCR
Reaction Speed:
Standard
Shipping Condition:
Wet or Dry Ice
Concentration:
10X
Hot Start:
Built-In Hot Start
Overhang:
3'-A
Size (Final Product):
5 kb or less
Content And Storage: Storage conditions: 2-8° C. Protect from lightShipping conditions: AmbientShelf life: 12 months from date of manufacture
GC-Rich PCR Performance: __
Polymerase: __
Product Type: Medium 199
For Use With (Application): __
Fidelity (vs. Taq): __
No. of Reactions: __
Reaction Format: __
Detection Method: __
PCR Method: __
Reaction Speed: __
Shipping Condition: Room Temperature
Concentration: 1 X
Hot Start: __
Overhang: __
Size (Final Product): __
Content And Storage:
Storage conditions: 2-8° C. Protect from lightShipping conditions: AmbientShelf life: 12 months from date of manufacture
GC-Rich PCR Performance:
__
Polymerase:
__
Product Type:
Medium 199
For Use With (Application):
__
Fidelity (vs. Taq):
__
No. of Reactions:
__
Reaction Format:
__
Detection Method:
__
PCR Method:
__
Reaction Speed:
__
Shipping Condition:
Room Temperature
Concentration:
1 X
Hot Start:
__
Overhang:
__
Size (Final Product):
__
Content And Storage: __
GC-Rich PCR Performance: __
Polymerase: __
Product Type: __
For Use With (Application): __
Fidelity (vs. Taq): __
No. of Reactions: __
Reaction Format: __
Detection Method: __
PCR Method: __
Reaction Speed: __
Shipping Condition: __
Concentration: __
Hot Start: __
Overhang: __
Size (Final Product): __
Content And Storage:
__
GC-Rich PCR Performance:
__
Polymerase:
__
Product Type:
__
For Use With (Application):
__
Fidelity (vs. Taq):
__
No. of Reactions:
__
Reaction Format:
__
Detection Method:
__
PCR Method:
__
Reaction Speed:
__
Shipping Condition:
__
Concentration:
__
Hot Start:
__
Overhang:
__
Size (Final Product):
__
Content And Storage: AccuPrime SuperMix II is supplied at 2X concentration to allow approximately 50% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 200 amplification reactions of 25 μL each are provided. AccuPrime SuperMix II Components: 40 mM Tris-HCl (pH 8.4); 100 mM KCl; 3 mM MgCl2, 400 μM each dGTP, dATP, dTTP, dCTP; AccuPrime Taq DNA Polymerase; thermostable AccuPrime protein; stabilizers.200 Reactions: 2 x 1.25 mL.Store at -20°C in a non-frost-free freezer.
GC-Rich PCR Performance: __
Polymerase: __
Product Type: __
For Use With (Application): __
Fidelity (vs. Taq): 2X
No. of Reactions: 200 Reactions
Reaction Format: __
Detection Method: __
PCR Method: __
Reaction Speed: __
Shipping Condition: __
Concentration: __
Hot Start: __
Overhang: __
Size (Final Product): __
Content And Storage:
AccuPrime SuperMix II is supplied at 2X concentration to allow approximately 50% of the final reaction volume to be used for the addition of primer and template solutions. Reagents sufficient for 200 amplification reactions of 25 μL each are provided. AccuPrime SuperMix II Components: 40 mM Tris-HCl (pH 8.4); 100 mM KCl; 3 mM MgCl2, 400 μM each dGTP, dATP, dTTP, dCTP; AccuPrime Taq DNA Polymerase; thermostable AccuPrime protein; stabilizers.200 Reactions: 2 x 1.25 mL.Store at -20°C in a non-frost-free freezer.
GC-Rich PCR Performance:
__
Polymerase:
__
Product Type:
__
For Use With (Application):
__
Fidelity (vs. Taq):
2X
No. of Reactions:
200 Reactions
Reaction Format:
__
Detection Method:
__
PCR Method:
__
Reaction Speed:
__
Shipping Condition:
__
Concentration:
__
Hot Start:
__
Overhang:
__
Size (Final Product):
__