4919378

Invitrogen™ AccuPrime™TaqDNA Polymerase System

The AccuPrimeTaqDNA Polymerase System provides reagents for amplification of nucleic acid templates with an antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases.

Manufacturer: Fischer Scientific

The price for this product is unavailable. Please request a quote

Detection Method

Primer-probe

PCR Method

1-step RT-qPCR, Multiplex qPCR

Reaction Speed

Standard

Shipping Condition

Wet or Dry Ice

Concentration

10X

Hot Start

Built-In Hot Start

Reaction Format

Separate Components

GC-Rich PCR Performance

Low

Polymerase

AccuPrime Taq DNA Polymerase

Product Type

DNA Polymerase System

For Use With (Application)

Hot-start PCR

Fidelity (vs. Taq)

2X

Overhang

3'-A

Size (Final Product)

5 kb or less

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Description

  • The AccuPrime Taq DNA Polymerase System provides reagents for amplification of nucleic acid templates with an antibody-mediated, hot-start protocol for improved PCR specificity over other hot-start DNA polymerases
  • Platinum anti- Taq DNA polymerase antibodies inhibit polymerase activity, providing an automatic hot-start, while a thermostable accessory protein enhances specific primer-template hybridization during every cycle of PCR
  • This combination improves the fidelity of Taq DNA Polymerase by two-fold and is ideal for high-throughput screening and multiplex PCR
  • AccuPrime Taq DNA Polymerase broadens primer annealing temperatures, giving you optimal performance between 55°C and 65°C
  • AccuPrime Taq DNA Polymerase features Control mispriming at every cycle for single-band PCR products Minimal reaction optimization and primer set redesign Reaction assembly at room temperature Applications AccuPrime Taq DNA Polymerase can be used for a wide variety of applications, including: Multiplex PCR TOPO TA Cloning Allele-specific amplifications High-throughput screening AccuPrime buffers The 10X AccuPrime buffers contain thermostable AccuPrime protein, Mg ++ , and deoxyribonucleotide triphosphates at concentrations sufficient to allow amplification during PCR
  • Two individual buffer systems (10X AccuPrime PCR Buffer I and II) are provided for amplification of specific types of templates
  • 10X AccuPrime PCR Buffer I is designed for small genomic DNA amplicons (≤200 bp), cDNA, or plasmids
  • 10X AccuPrime PCR Buffer II is designed for genomic DNA (200 bp–4 kb)
  • Order Info Shipping Condition: Approved for shipment on wet or dry ice

Compare Similar Items

Show Difference

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Fischer Scientific

4919378

The AccuPrimeTaqDNA Polymerase Syst...


Detection Method:
Primer-probe

PCR Method:
1-step RT-qPCR, Multiplex qPCR

Reaction Speed:
Standard

Shipping Condition:
Wet or Dry Ice

Concentration:
10X

Hot Start:
Built-In Hot Start

Reaction Format:
Separate Components

GC-Rich PCR Performance:
Low

Polymerase:
AccuPrime Taq DNA Polymerase

Product Type:
DNA Polymerase System

For Use With (Application):
Hot-start PCR

Fidelity (vs. Taq):
2X

Overhang:
3'-A

Size (Final Product):
5 kb or less

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Detection Method:
__

PCR Method:
__

Reaction Speed:
Standard

Shipping Condition:
Wet or Dry Ice

Concentration:
5 U/μL

Hot Start:
No

Reaction Format:
Separate Components

GC-Rich PCR Performance:
Low

Polymerase:
TaqDNA Polymerase

Product Type:
DNA Polymerase

For Use With (Application):
Standard PCR

Fidelity (vs. Taq):
1X

Overhang:
3'-A

Size (Final Product):
5 kb or less

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Detection Method:
Primer-probe

PCR Method:
2-step RT-qPCR

Reaction Speed:
Standard

Shipping Condition:
Dry Ice

Concentration:
__

Hot Start:
__

Reaction Format:
__

GC-Rich PCR Performance:
High

Polymerase:
DNA Polymerase

Product Type:
Gene Expression Cells-to-C Kit

For Use With (Application):
__

Fidelity (vs. Taq):
__

Overhang:
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Size (Final Product):
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Detection Method:
TaqMan

PCR Method:
1-step RT-qPCR

Reaction Speed:
7 min.

Shipping Condition:
__

Concentration:
__

Hot Start:
__

Reaction Format:
__

GC-Rich PCR Performance:
High

Polymerase:
__

Product Type:
1-Step qPCR kit

For Use With (Application):
Gene Expression

Fidelity (vs. Taq):
__

Overhang:
__

Size (Final Product):
__