Invitrogen™ AccuPrime™PfxDNA Polymerase

Description

• AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis
• High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading (3'→5' exonuclease) activity
• Platinum anti- Pfx DNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity (see figure)
• Thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over Platinum Pfx alone
• Benefits of AccuPrime Pfx DNA Polymerase Greater than 26 times higher fidelity than Taq DNA polymerase Amplification of fragments up to 12 kb Minimized PCR optimization Applications Amplification of DNA from complex genomic, viral, and plasmid templates RT-PCR High-fidelity PCR Hot-start PCR Notes AccuPrime Pfx DNA Polymerase is especially effective where very high levels of fidelity are required
• The 10X AccuPrime Pfx reaction mix contains MgSO 4 , dNTPs, and thermostable proteins that enhance specific primer-template hybridization during each PCR cycle
• Order Info Approved for shipment on Wet or Dry Ice