Invitrogen™ AccuPrime™ GC-Rich DNA Polymerase

Description

• AccuPrime GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content
• The kit offers a choice of buffers for amplifying genomic DNA targets (Buffer A) or non-GC-rich cDNA, plasmid, and lambda-based targets (Buffer B)
• AccuPrime GC-Rich DNA Polymerase features High yields for targets up to 5 kb in length AccuPrime accessory proteins for improved PCR specificity Sensitivity down to 5 ng of template DNA Robust and specific amplifications The AccuPrime buffers contain thermostable proteins that enhance primer-template hybridization during PCR, increasing the specificity of the reaction
• The polymerase itself, from the archaebacterium Pyrolobus fumarius , has a five-fold better processivity than Taq DNA polymerase, and remains active even after 4 hours at 95°C
• Unit definition One unit of AccuPrime GC-Rich DNA Polymerase is the amount of enzyme required to incorporate 10 nmoles of dNTPs into acid-insoluble material in 30 min
• at 74°C
• Order Info Approved for shipment on Wet or Dry Ice