12-344-024
Invitrogen™ AccuPrime™PfxDNA Polymerase
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 12-344-024-Each-of-1 | In Stock | ₹ 51,086.00 |
12-344-024 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 51,086.00
GST (18%): ₹ 9,195.48
Total Price: ₹ 60,281.48
Content And Storage
• AccuPrime Pfx DNA Polymerase (1 x 100 μL at 2.5 U/μL)• 10X AccuPrime Pfx Reaction Mix (1 x 1 mL)• 50 mM MgSO4(1 x 1 mL)Store at -20°C.
GC-Rich PCR Performance
Low
Reaction Speed
Standard
Shipping Condition
Wet or Dry Ice
Fidelity (vs. Taq)
26X
No. of Reactions
200 Reactions
Reaction Format
Separate Components
Volume
100 μL
Detection Method
Primer-probe
Polymerase
AccuPrime Pfx DNA Polymerase
Quantity
200 Reactions
For Use With (Application)
Hot-start PCR, High-fidelity PCR
Hot Start
Built-In Hot Start
Overhang
Blunt
Size (Final Product)
12 kb or less
Related Products
Description
- AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis
- High fidelity is provided by a proprietary enzyme preparation containing recombinant DNA polymerase from Thermococcus species KOD with proofreading (3'→5' exonuclease) activity
- Platinum anti- Pfx DNA polymerase antibodies inhibit polymerase activity, providing hot-start capabilities for improved PCR specificity (see figure)
- Thermostable AccuPrime accessory proteins enhance specific primer-template hybridization during every cycle of PCR, increasing specificity, yield, and robustness over Platinum Pfx alone
- Benefits of AccuPrime Pfx DNA Polymerase Greater than 26 times higher fidelity than Taq DNA polymerase Amplification of fragments up to 12 kb Minimized PCR optimization Applications Amplification of DNA from complex genomic, viral, and plasmid templates RT-PCR High-fidelity PCR Hot-start PCR Notes AccuPrime Pfx DNA Polymerase is especially effective where very high levels of fidelity are required
- The 10X AccuPrime Pfx reaction mix contains MgSO 4 , dNTPs, and thermostable proteins that enhance specific primer-template hybridization during each PCR cycle
- Order Info Approved for shipment on Wet or Dry Ice
Compare Similar Items
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Content And Storage: • AccuPrime Pfx DNA Polymerase (1 x 100 μL at 2.5 U/μL)• 10X AccuPrime Pfx Reaction Mix (1 x 1 mL)• 50 mM MgSO4(1 x 1 mL)Store at -20°C.
GC-Rich PCR Performance: Low
Reaction Speed: Standard
Shipping Condition: Wet or Dry Ice
Fidelity (vs. Taq): 26X
No. of Reactions: 200 Reactions
Reaction Format: Separate Components
Volume: 100 μL
Detection Method: Primer-probe
Polymerase: AccuPrime Pfx DNA Polymerase
Quantity: 200 Reactions
For Use With (Application): Hot-start PCR, High-fidelity PCR
Hot Start: Built-In Hot Start
Overhang: Blunt
Size (Final Product): 12 kb or less
Content And Storage:
• AccuPrime Pfx DNA Polymerase (1 x 100 μL at 2.5 U/μL)• 10X AccuPrime Pfx Reaction Mix (1 x 1 mL)• 50 mM MgSO4(1 x 1 mL)Store at -20°C.
GC-Rich PCR Performance:
Low
Reaction Speed:
Standard
Shipping Condition:
Wet or Dry Ice
Fidelity (vs. Taq):
26X
No. of Reactions:
200 Reactions
Reaction Format:
Separate Components
Volume:
100 μL
Detection Method:
Primer-probe
Polymerase:
AccuPrime Pfx DNA Polymerase
Quantity:
200 Reactions
For Use With (Application):
Hot-start PCR, High-fidelity PCR
Hot Start:
Built-In Hot Start
Overhang:
Blunt
Size (Final Product):
12 kb or less
Content And Storage: • AccuPrime Pfx DNA Polymerase (1 x 500 μL at 2.5 U/μL)• 10X AccuPrime Pfx Reaction Mix (5 x 1 mL)• 50 mM MgSO4(2 x 1 mL)Store at -20°C.
GC-Rich PCR Performance: Low
Reaction Speed: Standard
Shipping Condition: Wet or Dry Ice
Fidelity (vs. Taq): 26X
No. of Reactions: 1000 Reactions
Reaction Format: Separate Components
Volume: 500 μL
Detection Method: Primer-probe
Polymerase: AccuPrime Pfx DNA Polymerase
Quantity: __
For Use With (Application): Hot-start PCR, High-fidelity PCR
Hot Start: Built-In Hot Start
Overhang: Blunt
Size (Final Product): 12 kb or less
Content And Storage:
• AccuPrime Pfx DNA Polymerase (1 x 500 μL at 2.5 U/μL)• 10X AccuPrime Pfx Reaction Mix (5 x 1 mL)• 50 mM MgSO4(2 x 1 mL)Store at -20°C.
GC-Rich PCR Performance:
Low
Reaction Speed:
Standard
Shipping Condition:
Wet or Dry Ice
Fidelity (vs. Taq):
26X
No. of Reactions:
1000 Reactions
Reaction Format:
Separate Components
Volume:
500 μL
Detection Method:
Primer-probe
Polymerase:
AccuPrime Pfx DNA Polymerase
Quantity:
__
For Use With (Application):
Hot-start PCR, High-fidelity PCR
Hot Start:
Built-In Hot Start
Overhang:
Blunt
Size (Final Product):
12 kb or less
Content And Storage: • AccuPrime Taq DNA Polymerase, High Fidelity (1 x 40 μL at 5 U/μL)• 10X AccuPrime PCR Buffer I (1 x 1 mL)• 10X AccuPrime PCR Buffer II (1 x 1 mL)• 50 mM MgSO4(1 x 1 mL)Store at -10°C to -30°C.
GC-Rich PCR Performance: Low
Reaction Speed: Standard
Shipping Condition: Dry Ice
Fidelity (vs. Taq): 9X
No. of Reactions: 200 Reactions
Reaction Format: Separate Components
Volume: __
Detection Method: Primer-probe
Polymerase: AccuPrime Taq DNA Polymerase High Fidelity
Quantity: __
For Use With (Application): Hot-start PCR, High-fidelity PCR
Hot Start: Built-In Hot Start
Overhang: Mixed
Size (Final Product): 20 kb or less
Content And Storage:
• AccuPrime Taq DNA Polymerase, High Fidelity (1 x 40 μL at 5 U/μL)• 10X AccuPrime PCR Buffer I (1 x 1 mL)• 10X AccuPrime PCR Buffer II (1 x 1 mL)• 50 mM MgSO4(1 x 1 mL)Store at -10°C to -30°C.
GC-Rich PCR Performance:
Low
Reaction Speed:
Standard
Shipping Condition:
Dry Ice
Fidelity (vs. Taq):
9X
No. of Reactions:
200 Reactions
Reaction Format:
Separate Components
Volume:
__
Detection Method:
Primer-probe
Polymerase:
AccuPrime Taq DNA Polymerase High Fidelity
Quantity:
__
For Use With (Application):
Hot-start PCR, High-fidelity PCR
Hot Start:
Built-In Hot Start
Overhang:
Mixed
Size (Final Product):
20 kb or less
Content And Storage: • AccuPrime Taq DNA Polymerase, High Fidelity (1 x 200 μL at 5 U/μL)• 10X AccuPrime PCR Buffer I (4 x 1.25 mL)• 10X AccuPrime PCR Buffer II (4 x 1.25 mL)• 50 mM MgSO4(4 x 1.25 mL)Store at -10°C to -30°C.
GC-Rich PCR Performance: Low
Reaction Speed: Standard
Shipping Condition: Dry Ice
Fidelity (vs. Taq): 9X
No. of Reactions: 1000 Reactions
Reaction Format: Separate Components
Volume: __
Detection Method: Primer-probe
Polymerase: AccuPrime Taq DNA Polymerase High Fidelity
Quantity: __
For Use With (Application): Hot-start PCR, High-fidelity PCR
Hot Start: Built-In Hot Start
Overhang: Mixed, 3'-A
Size (Final Product): 20 kb or less
Content And Storage:
• AccuPrime Taq DNA Polymerase, High Fidelity (1 x 200 μL at 5 U/μL)• 10X AccuPrime PCR Buffer I (4 x 1.25 mL)• 10X AccuPrime PCR Buffer II (4 x 1.25 mL)• 50 mM MgSO4(4 x 1.25 mL)Store at -10°C to -30°C.
GC-Rich PCR Performance:
Low
Reaction Speed:
Standard
Shipping Condition:
Dry Ice
Fidelity (vs. Taq):
9X
No. of Reactions:
1000 Reactions
Reaction Format:
Separate Components
Volume:
__
Detection Method:
Primer-probe
Polymerase:
AccuPrime Taq DNA Polymerase High Fidelity
Quantity:
__
For Use With (Application):
Hot-start PCR, High-fidelity PCR
Hot Start:
Built-In Hot Start
Overhang:
Mixed, 3'-A
Size (Final Product):
20 kb or less