18-064-022
Invitrogen™ SuperScript™ II Reverse Transcriptase
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 18-064-022-Each-of-1 | In Stock | ₹ 10,858.00 |
18-064-022 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 10,858.00
GST (18%): ₹ 1,954.44
Total Price: ₹ 12,812.44
Content And Storage
• SuperScript II Reverse Transcriptase, 10 μL (1 x 2,000 units at 200 U/μL)• 5X First-strand buffer• 100 mM DTTStore at –20°C.
GC-Rich PCR Performance
High
Technique
Reverse Transcription
Quantity
2,000 Units
Ribonuclease H Activity
Reduced
Concentration
200 U/μL
No. of Reactions
10 Reactions
Reagent Type
Reverse Transcription
Starting Material
RNA
Format
Stand-alone Enzyme
Reaction Speed
50 min.
Optimal Reaction Temperature
42°C
Reverse Transcriptase
SuperScript II
Shipping Condition
Wet Ice
Final Product Type
First-Strand cDNA
Reaction Format
Separate components
Size (Final Product)
Up to 12.3 kb
Related Products
Description
- Invitrogen SuperScript II Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) with reduced RNase H activity and increased thermal stability compared to wild-type MMLV RT
- Mutations in the RNase H domain of the enzyme eliminate degradation of the RNA during first-strand cDNA synthesis, which results in higher yields of full-length cDNA
- SuperScript reverse transcriptases are the most highly trusted and widely used RTs with over 50,000 citations, reviews, and publications to date. The enzyme is purified to near homogeneity from E
- coli containing the modified pol gene of Moloney Murine Leukemia Virus
- Features of SuperScript II Reverse Transcriptase include: • High cDNA yields and more full-length product • Generation of cDNA up to 12.3 kb • First-strand cDNA synthesis at higher temperatures than conventional MMLV RT Note: The latest member of the SuperScript RT family, SuperScript IV Reverse Transcriptase (Cat
- No
- 18090010), features enhanced thermostability, processivity, yields, and performance with any RNA samples, including those of suboptimal purity or integrity
- Order Info Shipping Condition: Wet ice
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Content And Storage: • SuperScript II Reverse Transcriptase, 10 μL (1 x 2,000 units at 200 U/μL)• 5X First-strand buffer• 100 mM DTTStore at –20°C.
GC-Rich PCR Performance: High
Technique: Reverse Transcription
Quantity: 2,000 Units
Ribonuclease H Activity: Reduced
Concentration: 200 U/μL
No. of Reactions: 10 Reactions
Reagent Type: Reverse Transcription
Starting Material: RNA
Format: Stand-alone Enzyme
Reaction Speed: 50 min.
Optimal Reaction Temperature: 42°C
Reverse Transcriptase: SuperScript II
Shipping Condition: Wet Ice
Final Product Type: First-Strand cDNA
Reaction Format: Separate components
Size (Final Product): Up to 12.3 kb
Content And Storage:
• SuperScript II Reverse Transcriptase, 10 μL (1 x 2,000 units at 200 U/μL)• 5X First-strand buffer• 100 mM DTTStore at –20°C.
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription
Quantity:
2,000 Units
Ribonuclease H Activity:
Reduced
Concentration:
200 U/μL
No. of Reactions:
10 Reactions
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Format:
Stand-alone Enzyme
Reaction Speed:
50 min.
Optimal Reaction Temperature:
42°C
Reverse Transcriptase:
SuperScript II
Shipping Condition:
Wet Ice
Final Product Type:
First-Strand cDNA
Reaction Format:
Separate components
Size (Final Product):
Up to 12.3 kb
Content And Storage: • SuperScript II Reverse Transcriptase, 4 x 50 μL (4 x 10,000 units at 200 U/μL)• 5X First-strand buffer• DTT (100 mM)Store at –20°C.
GC-Rich PCR Performance: High
Technique: Reverse Transcription
Quantity: __
Ribonuclease H Activity: Reduced
Concentration: 200 U/μL
No. of Reactions: 200 Reactions
Reagent Type: Reverse Transcription
Starting Material: RNA
Format: Stand-alone Enzyme
Reaction Speed: 50 min.
Optimal Reaction Temperature: 42°C
Reverse Transcriptase: SuperScript II
Shipping Condition: Wet Ice
Final Product Type: First-Strand cDNA
Reaction Format: Separate components
Size (Final Product): Up to 12.3 kb
Content And Storage:
• SuperScript II Reverse Transcriptase, 4 x 50 μL (4 x 10,000 units at 200 U/μL)• 5X First-strand buffer• DTT (100 mM)Store at –20°C.
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription
Quantity:
__
Ribonuclease H Activity:
Reduced
Concentration:
200 U/μL
No. of Reactions:
200 Reactions
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Format:
Stand-alone Enzyme
Reaction Speed:
50 min.
Optimal Reaction Temperature:
42°C
Reverse Transcriptase:
SuperScript II
Shipping Condition:
Wet Ice
Final Product Type:
First-Strand cDNA
Reaction Format:
Separate components
Size (Final Product):
Up to 12.3 kb
Content And Storage: • 10X Taq DNA Polymerase PCR Buffer, 2 x 1 mL• 50 mM MgCl2, 1 mLStore at -20°C.Guaranteed stable for 6 months when properly stored.
GC-Rich PCR Performance: __
Technique: __
Quantity: 2 x 1 mL
Ribonuclease H Activity: __
Concentration: 10X
No. of Reactions: __
Reagent Type: __
Starting Material: __
Format: __
Reaction Speed: __
Optimal Reaction Temperature: __
Reverse Transcriptase: __
Shipping Condition: Dry Ice
Final Product Type: __
Reaction Format: Kit
Size (Final Product): __
Content And Storage:
• 10X Taq DNA Polymerase PCR Buffer, 2 x 1 mL• 50 mM MgCl2, 1 mLStore at -20°C.Guaranteed stable for 6 months when properly stored.
GC-Rich PCR Performance:
__
Technique:
__
Quantity:
2 x 1 mL
Ribonuclease H Activity:
__
Concentration:
10X
No. of Reactions:
__
Reagent Type:
__
Starting Material:
__
Format:
__
Reaction Speed:
__
Optimal Reaction Temperature:
__
Reverse Transcriptase:
__
Shipping Condition:
Dry Ice
Final Product Type:
__
Reaction Format:
Kit
Size (Final Product):
__
Content And Storage: Contents:1 vial of DNase I, Amp Grade (100 U)1 vial of 10X DNase I Reaction Buffer (1000 μL)1 vial of 25 mM EDTA (pH 8.0) (200 μL)Store at -20°C in a non-frost-free freezer.
GC-Rich PCR Performance: __
Technique: __
Quantity: 100 U
Ribonuclease H Activity: __
Concentration: __
No. of Reactions: __
Reagent Type: __
Starting Material: __
Format: __
Reaction Speed: __
Optimal Reaction Temperature: __
Reverse Transcriptase: __
Shipping Condition: Approved for shipment on Wet or Dry Ice
Final Product Type: __
Reaction Format: __
Size (Final Product): __
Content And Storage:
Contents:1 vial of DNase I, Amp Grade (100 U)1 vial of 10X DNase I Reaction Buffer (1000 μL)1 vial of 25 mM EDTA (pH 8.0) (200 μL)Store at -20°C in a non-frost-free freezer.
GC-Rich PCR Performance:
__
Technique:
__
Quantity:
100 U
Ribonuclease H Activity:
__
Concentration:
__
No. of Reactions:
__
Reagent Type:
__
Starting Material:
__
Format:
__
Reaction Speed:
__
Optimal Reaction Temperature:
__
Reverse Transcriptase:
__
Shipping Condition:
Approved for shipment on Wet or Dry Ice
Final Product Type:
__
Reaction Format:
__
Size (Final Product):
__