18-090-200
Invitrogen™ SuperScript™ IV Reverse Transcriptase
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 18-090-200-Each-of-1 | In Stock | ₹ 1,47,847.68 |
18-090-200 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,47,847.68
GST (18%): ₹ 26,612.582
Total Price: ₹ 1,74,460.262
Content And Storage
• SuperScript IV RT, 4 x 50 μL (200 U/μL)• 5X RT buffer, 4 x 1000 μL• 0.1 M DTT, 4 x 500 μLStore at -15°C to -25°C.
GC-Rich PCR Performance
High
Technique
Reverse Transcription
Quantity
4 x 10,000 Units
Ribonuclease H Activity
Reduced
Concentration
200 U/μL
No. of Reactions
200 Reactions
Reagent Type
Reverse Transcription
Starting Material
RNA
Format
Stand-alone Enzyme
Reaction Speed
10 min.
Optimal Reaction Temperature
50°C
Reverse Transcriptase
SuperScript IV
Shipping Condition
Dry Ice
Final Product Type
First-Strand cDNA
Reaction Format
Separate components
Size (Final Product)
Up to 12.3 kb
Related Products
Description
- SuperScript IV Reverse Transcriptase (RT) is a proprietary MMLV mutant with superior robustness and reliability in RT reactions
- It is significantly improved over SuperScript III RT in inhibitor resistance, processivity, and reaction speed, while retaining all the benefits of that enzyme, including increased thermostability, highly efficient full-length cDNA synthesis, and reduced RNase activity
- SuperScript IV RT is designed to provide reliable, consistent, and fast cDNA synthesis in the presence of inhibitors found in a wide variety of samples that cause other currently available RTs to perform inefficiently
- SuperScript IV RT offers advantages at standard as well as elevated temperatures over cloned AMV and ThermoScript RT, including higher sensitivity, inhibitor tolerance, and ability to work with degraded RNA
- Features of SuperScript IV Reverse Transcriptase include: • Significantly improved resistance to inhibitors that can interfere with cDNA synthesis • Robust and specific cDNA synthesis in a wide range of sample types • Faster reverse transcriptase reaction reduces incubation time from >50 minutes to 10 minutes • Better processivity when compared to SuperScript III RT SuperScript IV RT is the top choice for all RT-PCR and qRT-PCR applications in the SuperScript product family when reproducibility and reliability is the primary concern
- It is also our top performing RT when inhibitors in the RNA sample may interfere with cDNA synthesis, leading to biases in gene expression studies
- Source Purified from E
- coli expressing the pol gene of M-MLV, modified to increase thermostability and half-life, processivity, inhibitor resistance and to reduce RNase H activity Unit definition One unit of SuperScript IV RT is the amount of enzyme required to incorporate 1 nmole of deoxyribonucleotide into acid-precipitable material in 10 min at 37°C using poly(A) oligo(dT) 12-18 as a template/primer
- Unit reaction conditions 50 mM Tris-HCl (pH 8.3), 4 mM MgCl 2 , 10 mM DTT, 50 mM KCl, 0.5 mM dTTP, 0.4 MBq/mL [ 3 H]-dTTP, 0.4 mM poly(A) oligo (dT) 12-18 and enzyme in 20 μl for 10 min at 37°C.
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Content And Storage: • SuperScript IV RT, 4 x 50 μL (200 U/μL)• 5X RT buffer, 4 x 1000 μL• 0.1 M DTT, 4 x 500 μLStore at -15°C to -25°C.
GC-Rich PCR Performance: High
Technique: Reverse Transcription
Quantity: 4 x 10,000 Units
Ribonuclease H Activity: Reduced
Concentration: 200 U/μL
No. of Reactions: 200 Reactions
Reagent Type: Reverse Transcription
Starting Material: RNA
Format: Stand-alone Enzyme
Reaction Speed: 10 min.
Optimal Reaction Temperature: 50°C
Reverse Transcriptase: SuperScript IV
Shipping Condition: Dry Ice
Final Product Type: First-Strand cDNA
Reaction Format: Separate components
Size (Final Product): Up to 12.3 kb
Content And Storage:
• SuperScript IV RT, 4 x 50 μL (200 U/μL)• 5X RT buffer, 4 x 1000 μL• 0.1 M DTT, 4 x 500 μLStore at -15°C to -25°C.
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription
Quantity:
4 x 10,000 Units
Ribonuclease H Activity:
Reduced
Concentration:
200 U/μL
No. of Reactions:
200 Reactions
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Format:
Stand-alone Enzyme
Reaction Speed:
10 min.
Optimal Reaction Temperature:
50°C
Reverse Transcriptase:
SuperScript IV
Shipping Condition:
Dry Ice
Final Product Type:
First-Strand cDNA
Reaction Format:
Separate components
Size (Final Product):
Up to 12.3 kb
Content And Storage: • Oligo(dT)20, 50 μL (50 μM)• Random hexamers, 250 μL (50 ng/μL)• 5X RT buffer, 1 mL• DTT, 250 μL (0.1 M)• dNTP mix, 250 μL (10 mM)• SuperScript IV RT, 50 μL (200 U/μL)• Ribonuclease Inhibitor, 100 μL (40 U/μL)•E. coliRNase H, 50 μL (2 U/μL)• DEPC-treated water, 1.2 mL• Total HeLa RNA, 20 μL (10 ng/μL)• Sense Control Primer, 25 μL (10 μM)• Antisense Control Primer, 25 μL (10 μM)Store at -15°C to -25°C.
GC-Rich PCR Performance: High
Technique: Reverse Transcription
Quantity: __
Ribonuclease H Activity: __
Concentration: __
No. of Reactions: 50 Reactions
Reagent Type: Reverse Transcription
Starting Material: RNA
Format: Kit
Reaction Speed: 10 min.
Optimal Reaction Temperature: 50°C
Reverse Transcriptase: SuperScript IV
Shipping Condition: Dry Ice
Final Product Type: First-Strand cDNA
Reaction Format: Separate components
Size (Final Product): Up to 12.3 kb
Content And Storage:
• Oligo(dT)20, 50 μL (50 μM)• Random hexamers, 250 μL (50 ng/μL)• 5X RT buffer, 1 mL• DTT, 250 μL (0.1 M)• dNTP mix, 250 μL (10 mM)• SuperScript IV RT, 50 μL (200 U/μL)• Ribonuclease Inhibitor, 100 μL (40 U/μL)•E. coliRNase H, 50 μL (2 U/μL)• DEPC-treated water, 1.2 mL• Total HeLa RNA, 20 μL (10 ng/μL)• Sense Control Primer, 25 μL (10 μM)• Antisense Control Primer, 25 μL (10 μM)Store at -15°C to -25°C.
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription
Quantity:
__
Ribonuclease H Activity:
__
Concentration:
__
No. of Reactions:
50 Reactions
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Format:
Kit
Reaction Speed:
10 min.
Optimal Reaction Temperature:
50°C
Reverse Transcriptase:
SuperScript IV
Shipping Condition:
Dry Ice
Final Product Type:
First-Strand cDNA
Reaction Format:
Separate components
Size (Final Product):
Up to 12.3 kb
Content And Storage: • Oligo(dT)20, 50 μL (50 μM)• Random hexamers, 250 μL (50 ng/μL)• 5X RT buffer, 1 mL• DTT, 250 μL (0.1 M)• dNTP mix, 250 μL (10 mM)• SuperScript IV RT, 50 μL (200 U/μL)• Ribonuclease Inhibitor, 100 μL (40 U/μL)•E. coliRNase H, 50 μL (2 U/μL)• DEPC-treated water, 1.2 mL• Total HeLa RNA, 20 μL (10 ng/μL)• Sense Control Primer, 25 μL (10 μM)• Antisense Control Primer, 25 μL (10 μM)• ezDNase Enzyme, 50 μL• 10X ezDNase Buffer, 100 μL• Nuclease-free water, 1.25 mLStore at -15°C to -25°C.
GC-Rich PCR Performance: High
Technique: Reverse Transcription
Quantity: __
Ribonuclease H Activity: __
Concentration: __
No. of Reactions: 50 Reactions
Reagent Type: Reverse Transcription
Starting Material: RNA
Format: Kit
Reaction Speed: 10 min.
Optimal Reaction Temperature: 50°C
Reverse Transcriptase: SuperScript IV
Shipping Condition: __
Final Product Type: First-Strand cDNA
Reaction Format: Separate components
Size (Final Product): Up to 12.3 kb
Content And Storage:
• Oligo(dT)20, 50 μL (50 μM)• Random hexamers, 250 μL (50 ng/μL)• 5X RT buffer, 1 mL• DTT, 250 μL (0.1 M)• dNTP mix, 250 μL (10 mM)• SuperScript IV RT, 50 μL (200 U/μL)• Ribonuclease Inhibitor, 100 μL (40 U/μL)•E. coliRNase H, 50 μL (2 U/μL)• DEPC-treated water, 1.2 mL• Total HeLa RNA, 20 μL (10 ng/μL)• Sense Control Primer, 25 μL (10 μM)• Antisense Control Primer, 25 μL (10 μM)• ezDNase Enzyme, 50 μL• 10X ezDNase Buffer, 100 μL• Nuclease-free water, 1.25 mLStore at -15°C to -25°C.
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription
Quantity:
__
Ribonuclease H Activity:
__
Concentration:
__
No. of Reactions:
50 Reactions
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Format:
Kit
Reaction Speed:
10 min.
Optimal Reaction Temperature:
50°C
Reverse Transcriptase:
SuperScript IV
Shipping Condition:
__
Final Product Type:
First-Strand cDNA
Reaction Format:
Separate components
Size (Final Product):
Up to 12.3 kb
Content And Storage: • Oligo(dT)20, 4 x 50 μL (50 μM)• Random hexamers, 4 x 250 μL (50 ng/μL)• 5X RT buffer, 4 x 1 mL• DTT, 4 x 250 μL (0.1 M)• dNTP mix, 250 μL (10 mM)• SuperScript IV RT, 4 x 50 μL (200 U/μL)• Ribonuclease Inhibitor, 4 x 100 μL (40 U/μL)•E. coliRNase H, 4 x 50 μL (2 U/μL)• DEPC-treated water, 4 x 1.2 mL• Total HeLa RNA, 4 x 20 μL (10 ng/μL)• Sense Control Primer, 4 x 25 μL (10 μM)• Antisense Control Primer, 4 x 25 μL (10 μM)Store at -15°C to -25°C.
GC-Rich PCR Performance: High
Technique: Reverse Transcription
Quantity: __
Ribonuclease H Activity: __
Concentration: __
No. of Reactions: 200 Reactions
Reagent Type: Reverse Transcription
Starting Material: RNA
Format: Kit
Reaction Speed: 10 min.
Optimal Reaction Temperature: 50°C
Reverse Transcriptase: SuperScript IV
Shipping Condition: Dry Ice
Final Product Type: First-Strand cDNA
Reaction Format: Separate components
Size (Final Product): Up to 12.3 kb
Content And Storage:
• Oligo(dT)20, 4 x 50 μL (50 μM)• Random hexamers, 4 x 250 μL (50 ng/μL)• 5X RT buffer, 4 x 1 mL• DTT, 4 x 250 μL (0.1 M)• dNTP mix, 250 μL (10 mM)• SuperScript IV RT, 4 x 50 μL (200 U/μL)• Ribonuclease Inhibitor, 4 x 100 μL (40 U/μL)•E. coliRNase H, 4 x 50 μL (2 U/μL)• DEPC-treated water, 4 x 1.2 mL• Total HeLa RNA, 4 x 20 μL (10 ng/μL)• Sense Control Primer, 4 x 25 μL (10 μM)• Antisense Control Primer, 4 x 25 μL (10 μM)Store at -15°C to -25°C.
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription
Quantity:
__
Ribonuclease H Activity:
__
Concentration:
__
No. of Reactions:
200 Reactions
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Format:
Kit
Reaction Speed:
10 min.
Optimal Reaction Temperature:
50°C
Reverse Transcriptase:
SuperScript IV
Shipping Condition:
Dry Ice
Final Product Type:
First-Strand cDNA
Reaction Format:
Separate components
Size (Final Product):
Up to 12.3 kb