18-080-085
Invitrogen™ SuperScript™ III Reverse Transcriptase
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 18-080-085-Each-of-1 | In Stock | ₹ 1,42,542.96 |
18-080-085 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 1,42,542.96
GST (18%): ₹ 25,657.733
Total Price: ₹ 1,68,200.693
Content And Storage
• SuperScript™ III Reverse Transcriptase, 4 x 50 μL (4 x 10,000 units at 200 U/μL)• 5X First-strand buffer, 4 x 1 mL• DTT, 4 x 500 μL (100 mM)Store at –20°C.
GC-Rich PCR Performance
High
Technique
Reverse Transcription
Reverse Transcriptase
SuperScript III
Shipping Condition
Dry Ice
Final Product Type
First-Strand cDNA
Reaction Format
Separate components
Size (Final Product)
Up to 12.3 kb
Format
Stand-alone Enzyme
Reaction Speed
30 to 50 min.
Optimal Reaction Temperature
50°C
Ribonuclease H Activity
Reduced
Concentration
200 U/μL
No. of Reactions
200 Reactions
Reagent Type
Reverse Transcription
Starting Material
RNA
Related Products
Description
- Invitrogen SuperScript III Reverse Transcriptase is a genetically engineered MMLV reverse transcriptase (RT) created by the introduction of several mutations for reduced RNase H activity, increased half-life, and improved thermal stability
- SuperScript III RT offers higher cDNA yields, improved cDNA lengths, improved efficiency on GC-rich target RNAs, and overall better performance than wild-type MMLV and MMLV RNase H-minus enzymes
- SuperScript RTs are the most highly trusted and widely used RTs with over 50,000 citations, reviews, and publications to date. The enzyme is purified to near homogeneity from E
- coli containing the modified pol gene of Moloney Murine Leukemia Virus
- Features of SuperScript III Reverse Transcriptase include: • First-strand cDNA may be synthesized at temperatures up to 55°C • cDNA can be generated from 100 bp to >12 kb • Increased specificity • High yields of cDNA and full-length products Note: The latest member of the SuperScript RT family, SuperScript IV Reverse Transcriptase (Cat
- No
- 18090010), features enhanced thermostability, processivity, yields, and performance with any RNA samples, including those of suboptimal purity or integrity
- Order Info Shipping Condition: Dry ice
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Content And Storage: • SuperScript™ III Reverse Transcriptase, 4 x 50 μL (4 x 10,000 units at 200 U/μL)• 5X First-strand buffer, 4 x 1 mL• DTT, 4 x 500 μL (100 mM)Store at –20°C.
GC-Rich PCR Performance: High
Technique: Reverse Transcription
Reverse Transcriptase: SuperScript III
Shipping Condition: Dry Ice
Final Product Type: First-Strand cDNA
Reaction Format: Separate components
Size (Final Product): Up to 12.3 kb
Format: Stand-alone Enzyme
Reaction Speed: 30 to 50 min.
Optimal Reaction Temperature: 50°C
Ribonuclease H Activity: Reduced
Concentration: 200 U/μL
No. of Reactions: 200 Reactions
Reagent Type: Reverse Transcription
Starting Material: RNA
Content And Storage:
• SuperScript™ III Reverse Transcriptase, 4 x 50 μL (4 x 10,000 units at 200 U/μL)• 5X First-strand buffer, 4 x 1 mL• DTT, 4 x 500 μL (100 mM)Store at –20°C.
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription
Reverse Transcriptase:
SuperScript III
Shipping Condition:
Dry Ice
Final Product Type:
First-Strand cDNA
Reaction Format:
Separate components
Size (Final Product):
Up to 12.3 kb
Format:
Stand-alone Enzyme
Reaction Speed:
30 to 50 min.
Optimal Reaction Temperature:
50°C
Ribonuclease H Activity:
Reduced
Concentration:
200 U/μL
No. of Reactions:
200 Reactions
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Content And Storage: • SuperScript™ III Reverse Transcriptase, 10 μL (2,000 units at 200 U/μL)• 5X First-strand buffer, 1 mL• DTT, 500 μL (100 mM)Store at –20°C.
GC-Rich PCR Performance: High
Technique: Reverse Transcription
Reverse Transcriptase: SuperScript III
Shipping Condition: Dry Ice
Final Product Type: First-Strand cDNA
Reaction Format: Separate components
Size (Final Product): Up to 12.3 kb
Format: Stand-alone Enzyme
Reaction Speed: 30 to 50 min.
Optimal Reaction Temperature: 50°C
Ribonuclease H Activity: Reduced
Concentration: 200 U/μL
No. of Reactions: 10 Reactions
Reagent Type: Reverse Transcription
Starting Material: RNA
Content And Storage:
• SuperScript™ III Reverse Transcriptase, 10 μL (2,000 units at 200 U/μL)• 5X First-strand buffer, 1 mL• DTT, 500 μL (100 mM)Store at –20°C.
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription
Reverse Transcriptase:
SuperScript III
Shipping Condition:
Dry Ice
Final Product Type:
First-Strand cDNA
Reaction Format:
Separate components
Size (Final Product):
Up to 12.3 kb
Format:
Stand-alone Enzyme
Reaction Speed:
30 to 50 min.
Optimal Reaction Temperature:
50°C
Ribonuclease H Activity:
Reduced
Concentration:
200 U/μL
No. of Reactions:
10 Reactions
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Content And Storage: • SuperScript III RT• RNaseOUT Ribonuclease Inhibitor• Oligo(dT)20primers• DNase I• DNase I buffer• EDTA• dNTP mix• 5X First-strand buffer• DTT (0.1 M)• RNase H• Control RNA & primers• Resuspension bufferStore at –20°C.Guaranteed stable for 6 months when properly stored.
GC-Rich PCR Performance: High
Technique: Reverse Transcription
Reverse Transcriptase: SuperScript III
Shipping Condition: __
Final Product Type: First-Strand cDNA
Reaction Format: Separate components
Size (Final Product): Up to 4.5 kb
Format: Kit
Reaction Speed: 50 min.
Optimal Reaction Temperature: 50°C
Ribonuclease H Activity: Reduced
Concentration: __
No. of Reactions: 25 Reactions
Reagent Type: Reverse Transcription
Starting Material: Cells
Content And Storage:
• SuperScript III RT• RNaseOUT Ribonuclease Inhibitor• Oligo(dT)20primers• DNase I• DNase I buffer• EDTA• dNTP mix• 5X First-strand buffer• DTT (0.1 M)• RNase H• Control RNA & primers• Resuspension bufferStore at –20°C.Guaranteed stable for 6 months when properly stored.
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription
Reverse Transcriptase:
SuperScript III
Shipping Condition:
__
Final Product Type:
First-Strand cDNA
Reaction Format:
Separate components
Size (Final Product):
Up to 4.5 kb
Format:
Kit
Reaction Speed:
50 min.
Optimal Reaction Temperature:
50°C
Ribonuclease H Activity:
Reduced
Concentration:
__
No. of Reactions:
25 Reactions
Reagent Type:
Reverse Transcription
Starting Material:
Cells
Content And Storage: • Resuspension Buffer, 1 mL• RNaseOUT™ Recombinant Ribonuclease Inhibitor, 200 μL (40 units/μL)• DNase I, 500 μL (1 U/μL)• 10X DNase I Buffer, 160 μL• 25 mM EDTA, 120 μL• Oligo(dT)20, 120 μL (50 μM)• 10 mM dNTP Mix, 100 μL• SuperScript™ III RT, 100 μL (200 units/μL)• 5X RT Buffer, 600 μL• DTT, 100 μL (0.1 M)•E. coliRNase H, 100 μL (2 U/μL)• HeLa Total RNA, 10 μL (10 ng/μL)• Forward/Reverse Control Primers, 10 μL (10 μM)•E. coliRNase H (2 U/μL)Store at –20°C.
GC-Rich PCR Performance: High
Technique: Reverse Transcription
Reverse Transcriptase: SuperScript III
Shipping Condition: __
Final Product Type: First-Strand cDNA
Reaction Format: Separate components
Size (Final Product): Up to 4.5 kb
Format: Kit
Reaction Speed: 50 min.
Optimal Reaction Temperature: 50°C
Ribonuclease H Activity: Reduced
Concentration: __
No. of Reactions: 100 Reactions
Reagent Type: Reverse Transcription
Starting Material: Cells
Content And Storage:
• Resuspension Buffer, 1 mL• RNaseOUT™ Recombinant Ribonuclease Inhibitor, 200 μL (40 units/μL)• DNase I, 500 μL (1 U/μL)• 10X DNase I Buffer, 160 μL• 25 mM EDTA, 120 μL• Oligo(dT)20, 120 μL (50 μM)• 10 mM dNTP Mix, 100 μL• SuperScript™ III RT, 100 μL (200 units/μL)• 5X RT Buffer, 600 μL• DTT, 100 μL (0.1 M)•E. coliRNase H, 100 μL (2 U/μL)• HeLa Total RNA, 10 μL (10 ng/μL)• Forward/Reverse Control Primers, 10 μL (10 μM)•E. coliRNase H (2 U/μL)Store at –20°C.
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription
Reverse Transcriptase:
SuperScript III
Shipping Condition:
__
Final Product Type:
First-Strand cDNA
Reaction Format:
Separate components
Size (Final Product):
Up to 4.5 kb
Format:
Kit
Reaction Speed:
50 min.
Optimal Reaction Temperature:
50°C
Ribonuclease H Activity:
Reduced
Concentration:
__
No. of Reactions:
100 Reactions
Reagent Type:
Reverse Transcription
Starting Material:
Cells