Invitrogen™ SuperScript™ III CellsDirect™ cDNA Synthesis Kit

Description

• The SuperScript III CellsDirect cDNA Synthesis Kit is optimized for synthesis of first-strand cDNA directly from a mammalian cell lysate without first isolating the RNA
• Lysis and reverse transcription are performed in the same tube, and the resulting first-strand cDNA is ready to use in cloning and PCR
• Features of the SuperScript III CellsDirect Synthesis Kit include: • Compatible with a wide range of mammalian cell types grown under different treatment conditions • Single-tube format minimizes reagent loss, sample loss, and handling time • Total lysate volume is used in first-strand cDNA synthesis reaction, providing greater yields with a limited number of cells and allowing for detection of rare transcripts • SuperScript III Reverse Transcriptase, with reduced RNase H activity and higher thermal stability, produces high yields of cDNA in the first-strand synthesis reaction, for greater sensitivity and enhanced detection of rare transcripts • Generates high-quality cDNA for use in a variety of applications, including cloning and PCR • Simple protocol takes less than two hours How it works In traditional RT-PCR, RNA is first isolated from cells in a time-consuming procedure that can lead to a loss of material
• Using the SuperScript III CellsDirect cDNA Synthesis Kit, the cells are lysed and the cDNA is generated from the lysate in a single tube with minimal handling and no sample loss
• DNase I is added to eliminate genomic DNA prior to first-strand synthesis
• This kit has been optimized for small cell samples, ranging from 10,000 cells down to a single cell (as measured by serial dilution)
• The use of SuperScript III Reverse Transcriptase ensures high specificity and high yields of cDNA from small amounts of starting material (as little as 10 pg total RNA)
• After synthesis, the first-strand cDNA can be amplified with specific primers by PCR without intermediate organic extractions or ethanol precipitations.