Applied Biosystems™ High-Capacity RNA-to-cDNA™ Kit

Description

• Description The High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with TaqMan Gene Expression Master Mix, Power SYBR Green PCR Master Mix, and other PCR enzymes. The components of the two-tube kit work together to provide sensitive and specific reverse transcription across a broad range of template amounts
• Features of the High Capacity RNA-to-cDNA Kit include: • Fast reaction time (typically 30–60 min) • Convenient and simple workflow with few pipetting steps • Reliable reverse transcription of both abundant and limited targets • Optimized two-step protocol enables multiple PCR reactions from a single RT reaction Streamline cDNA synthesis while maintaining detection sensitivity Amplification of a dilution series of the RNA concentration reference standard demonstrates exceptional reverse transcription and PCR efficiency across 11 orders of magnitude
• This wide dynamic range allows one set of reaction conditions to detect transcripts from highly active as well as weakly expressed genes
• The two-tube formulation reduces reaction time and requires fewer pipetting steps than with the High Capacity cDNA Reverse Transcription Kit, while maintaining high performance.