11-752-250
Invitrogen™ SuperScript™ III First-Strand Synthesis SuperMix for qRT-PCR
Manufacturer: Fischer Scientific
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 11-752-250-Each-of-1 | In Stock | ₹ 2,57,963.40 |
11-752-250 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 2,57,963.40
GST (18%): ₹ 46,433.412
Total Price: ₹ 3,04,396.812
Content And Storage
• 2X RT Reaction Mix (2 x 1.25 mL)• RT Enzyme Mix (500 μL)•E. coliRNase (250 μL)Sufficient reagents for 250 reactions at 20 μL reaction volumes.Store at –20°C.
Format
Super Mix
Reaction Speed
30 min.
Optimal Reaction Temperature
50°C
Shipping Condition
Dry Ice
Final Product Type
First-Strand cDNA
Reaction Format
Master Mix
Starting Material
RNA
Detection Method
qPCR
GC-Rich PCR Performance
High
Technique
Reverse Transcription
Reverse Transcriptase
SuperScript III
For Use With (Application)
Real Time PCR (qPCR)
No. of Reactions
250 Reactions
Reagent Type
Reverse Transcription
Related Products
Description
- SuperScript III First-Strand Synthesis SuperMix for qRT-PCR provides the high-temperature capability of SuperScript III Reverse Transcriptase in an optimized SuperMix format for the synthesis of first-strand cDNA for use in real-time quantitative RT-PCR (qRT-PCR)
- The simple, time-saving reaction set-up uses just two tubes: a 2X reaction mix and an enzyme mix
- Enzyme mix SuperScript III Reverse Transcriptase, included in the RT enzyme mix, is a version of M-MLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability
- The enzyme can be used to synthesize cDNA at a temperature range of 42–60°C, providing increased specificity, higher yields of cDNA, and more full-length product than other reverse transcriptases
- Because SuperScript III RT is not significantly inhibited by ribosomal and transfer RNA, it can be used to synthesize cDNA from total RNA
- RNaseOUT Recombinant Ribonuclease Inhibitor, also included in the enzyme mix, is an RNase inhibitor protein that safeguards against the degradation of target RNA due to ribonuclease contamination of the RNA preparation
- Reaction mix The 2X RT reaction mix includes oligo(dT) 20 , random hexamers, MgCl 2 , and dNTPs in a buffer formulation that has been optimized for qRT-PCR
- E
- coli RNase H is provided as a separate tube in the kit to remove the RNA template from the cDNA:RNA hybrid molecule after first-strand synthesis
- This has been shown to increase sensitivity in qRT-PCR
- Using SuperScript III First-Strand Synthesis SuperMix This SuperMix formulation can be used to quantify fewer than 10 copies of a target gene in qRT-PCR, with a broad dynamic range that supports accurate quantification of high-copy mRNA from up to 1 μg of total RNA
- Reagents are provided for 50 or 250 RT reactions of 20 μL each
- Order Info Shipping Condition: Dry ice
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Content And Storage: • 2X RT Reaction Mix (2 x 1.25 mL)• RT Enzyme Mix (500 μL)•E. coliRNase (250 μL)Sufficient reagents for 250 reactions at 20 μL reaction volumes.Store at –20°C.
Format: Super Mix
Reaction Speed: 30 min.
Optimal Reaction Temperature: 50°C
Shipping Condition: Dry Ice
Final Product Type: First-Strand cDNA
Reaction Format: Master Mix
Starting Material: RNA
Detection Method: qPCR
GC-Rich PCR Performance: High
Technique: Reverse Transcription
Reverse Transcriptase: SuperScript III
For Use With (Application): Real Time PCR (qPCR)
No. of Reactions: 250 Reactions
Reagent Type: Reverse Transcription
Content And Storage:
• 2X RT Reaction Mix (2 x 1.25 mL)• RT Enzyme Mix (500 μL)•E. coliRNase (250 μL)Sufficient reagents for 250 reactions at 20 μL reaction volumes.Store at –20°C.
Format:
Super Mix
Reaction Speed:
30 min.
Optimal Reaction Temperature:
50°C
Shipping Condition:
Dry Ice
Final Product Type:
First-Strand cDNA
Reaction Format:
Master Mix
Starting Material:
RNA
Detection Method:
qPCR
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription
Reverse Transcriptase:
SuperScript III
For Use With (Application):
Real Time PCR (qPCR)
No. of Reactions:
250 Reactions
Reagent Type:
Reverse Transcription
Content And Storage: Ten packs, each containing:• 10X Lysis Buffer (48 μL)• RNase Inhibitor (19 μL)• Capturing Oligonucleotide (48 μL)• 4X SuperScript IV cDNA Synthesis Master Mix (240 μL)• Template Switching Oligonucleotide (48 μL)• 2X Platinum SuperFi U Preamplification Master Mix (2 x 1.2 mL)• Preamplification primer (48 μL)• Nuclease-free water (2 x 1.25 mL)Store at –15 to –25°C.
Format: Kit
Reaction Speed: 30 min.
Optimal Reaction Temperature: 50°C
Shipping Condition: Dry Ice
Final Product Type: PCR Amplified cDNA
Reaction Format: __
Starting Material: Cells or RNA
Detection Method: __
GC-Rich PCR Performance: High
Technique: Reverse Transcription, Preamplification
Reverse Transcriptase: SuperScript IV
For Use With (Application): Next-Generation Sequencing (NGS), Real Time PCR (qPCR)
No. of Reactions: 480 Reactions
Reagent Type: Reverse Transcription
Content And Storage:
Ten packs, each containing:• 10X Lysis Buffer (48 μL)• RNase Inhibitor (19 μL)• Capturing Oligonucleotide (48 μL)• 4X SuperScript IV cDNA Synthesis Master Mix (240 μL)• Template Switching Oligonucleotide (48 μL)• 2X Platinum SuperFi U Preamplification Master Mix (2 x 1.2 mL)• Preamplification primer (48 μL)• Nuclease-free water (2 x 1.25 mL)Store at –15 to –25°C.
Format:
Kit
Reaction Speed:
30 min.
Optimal Reaction Temperature:
50°C
Shipping Condition:
Dry Ice
Final Product Type:
PCR Amplified cDNA
Reaction Format:
__
Starting Material:
Cells or RNA
Detection Method:
__
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription, Preamplification
Reverse Transcriptase:
SuperScript IV
For Use With (Application):
Next-Generation Sequencing (NGS), Real Time PCR (qPCR)
No. of Reactions:
480 Reactions
Reagent Type:
Reverse Transcription
Content And Storage: • 10 mL Resuspension Buffer• 1 mL Lysis Enhancer• 500 μL DNase I, Amplification Grade (1 U/μL)• 160 μL 10X DNase I Buffer• 400 μL 25 mM EDTA• 100 μL SuperScript™ III RT/Platinum™ Taq Mix• 2 × 1.25 mL 2X Reaction Mix• 1 mL 50 mM MgSO4• 2 mL DEPC-treated water• 10 μL HeLa Total RNA (10 ng/μL)• 100 μL ROX™ Reference Dye (store at -20°C in the dark)The kit is shipped on dry ice and should be stored at -20°C
Format: __
Reaction Speed: Fast or Standard
Optimal Reaction Temperature: __
Shipping Condition: __
Final Product Type: __
Reaction Format: __
Starting Material: __
Detection Method: Primer-probe
GC-Rich PCR Performance: __
Technique: __
Reverse Transcriptase: __
For Use With (Application): RT-PCR
No. of Reactions: 100 reactions
Reagent Type: __
Content And Storage:
• 10 mL Resuspension Buffer• 1 mL Lysis Enhancer• 500 μL DNase I, Amplification Grade (1 U/μL)• 160 μL 10X DNase I Buffer• 400 μL 25 mM EDTA• 100 μL SuperScript™ III RT/Platinum™ Taq Mix• 2 × 1.25 mL 2X Reaction Mix• 1 mL 50 mM MgSO4• 2 mL DEPC-treated water• 10 μL HeLa Total RNA (10 ng/μL)• 100 μL ROX™ Reference Dye (store at -20°C in the dark)The kit is shipped on dry ice and should be stored at -20°C
Format:
__
Reaction Speed:
Fast or Standard
Optimal Reaction Temperature:
__
Shipping Condition:
__
Final Product Type:
__
Reaction Format:
__
Starting Material:
__
Detection Method:
Primer-probe
GC-Rich PCR Performance:
__
Technique:
__
Reverse Transcriptase:
__
For Use With (Application):
RT-PCR
No. of Reactions:
100 reactions
Reagent Type:
__
Content And Storage: • 10 mL Resuspension Buffer• 1 mL Lysis Enhancer• 2 × 1.25 mL DNase I, Amplification Grade (1 U/μL)• 800 μL 10X DNase I Buffer• 2 × 1 mL 25 mM EDTA• 500 μL SuperScript™ III RT/Platinum™ Taq Mix• 12.5 mL 2X Reaction Mix• 1 mL 50 mM MgSO4• 12.5 mL DEPC-treated water• 10 μL HeLa Total RNA (10 ng/μL)• 500 μL ROX™ Reference Dye (store at -20°C in the dark)The kit is shipped on dry ice and should be stored at -20°C
Format: __
Reaction Speed: Fast or Standard
Optimal Reaction Temperature: __
Shipping Condition: __
Final Product Type: __
Reaction Format: __
Starting Material: __
Detection Method: Primer-probe
GC-Rich PCR Performance: __
Technique: __
Reverse Transcriptase: __
For Use With (Application): RT-PCR
No. of Reactions: 500 reactions
Reagent Type: __
Content And Storage:
• 10 mL Resuspension Buffer• 1 mL Lysis Enhancer• 2 × 1.25 mL DNase I, Amplification Grade (1 U/μL)• 800 μL 10X DNase I Buffer• 2 × 1 mL 25 mM EDTA• 500 μL SuperScript™ III RT/Platinum™ Taq Mix• 12.5 mL 2X Reaction Mix• 1 mL 50 mM MgSO4• 12.5 mL DEPC-treated water• 10 μL HeLa Total RNA (10 ng/μL)• 500 μL ROX™ Reference Dye (store at -20°C in the dark)The kit is shipped on dry ice and should be stored at -20°C
Format:
__
Reaction Speed:
Fast or Standard
Optimal Reaction Temperature:
__
Shipping Condition:
__
Final Product Type:
__
Reaction Format:
__
Starting Material:
__
Detection Method:
Primer-probe
GC-Rich PCR Performance:
__
Technique:
__
Reverse Transcriptase:
__
For Use With (Application):
RT-PCR
No. of Reactions:
500 reactions
Reagent Type:
__