Applied Biosystems™ MeltDoctor™ HRM Master Mix

Description

• High Resolution Melting (HRM) analysis is a post-PCR analysis method used to identify genetic variation in nucleic acid sequences
• Unlike some competitive mixes, the MeltDoctor™ HRM Master Mix does not require additional mixing prior to use, and was developed and optimized solely for HRM applications
• Low background fluorescence High brightness in the presence of double-stranded DNA Minimal temperature shift of DNA melting due to dye binding Thermal stability to tolerate PCR cycling conditions No inhibition of polymerase activity, resulting in high PCR efficiency A dNTP blend including dUTP, which minimizes carryover contamination by allowing amplicon degradation by uracil DNA glycosylase (UDG) in subsequent PCR reactions Perform Mutation Scanning: HRM analysis can be used to scan for mutations in target genes for the identification of variant samples prior to sequencing analysis As a mutation scanning technique, HRM offers significant advantages over conventional methods such as Denaturing High- Performance Liquid Chromatography (DHPLC) and Denaturing Gradient Gel Electrophoresis (DGGE) Advantages of HRM for mutation scanning Low reagent consumption, with little waste: HRM requires only a 20μL PCR reaction for analysis of each sample, eliminating the need for HPLC solvents or DGGE gels Simple, fast workflow: no additional instrumentation is required after PCR amplification;A high-resolution melt step can be simply added to the end of the PCR profile for immediate analysis Fast optimization: unlike DHPLC, thermal optimization is not required Low sample consumption: following HRM analysis, the PCR product can be used directly in a Sanger sequencing reaction Quality Components Magnesium salts and other buffer components, precisely formulated to obtain optimal HRM results AmpliTaq Gold™ 360 DNA Polymerase, a highly purified DNA polymerase that provides hot-start performance, minimizing non-specific product formation and enabling reactions to be set up at room temperature MeltDoctor™ HRM Dye, a stabilized form of the fluorescent SYTO™ 9 double-stranded nucleic acid stain developed by Molecular Probes Order Info Guaranteed minimum shelf life is 60 days
• Shipping Conditions: Wet Ice