Thermo Scientific™ GeneJET PCR Purification Kit

Description

• Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions
• It effectively removes primers, dNTPs, unincorporated labeled nucleotides, enzymes, and salts from PCR and other reaction mixtures
• The kit can be used for purification of DNA fragments from 25 bp to 20 kb with recovery rates up to 100%
• Each GeneJET purification column has a total binding capacity of up to 25 μg of DNA, and the entire procedure takes 5 minutes
• The purified DNA can be used in common downstream applications, such as sequencing, restriction digestion, labeling, ligation, cloning, in vitro transcription, blotting or in situ hybridization
• Highlights • Highly efficient: 90 to 100% recoveries in the range of 100 bp to 10 kb • Fast: procedure takes only 5 minutes • Convenient: spin columns are capped and assembled with collection tubes Applications Fast and efficient purification of DNA fragments ideal for use in all conventional molecular biology procedures including: • FastDigest or conventional restriction digestion • Automated fluorescent or radioactive sequencing • PCR • In vitro transcription • In situ hybridization • Labeling • Cloning