Thermo Scientific™ Phusion U Hot Start PCR Master Mix

Description

• Thermo Scientific Phusion U Hot Start PCR Master Mix is a convenient 2X formula that contains Phusion U Hot Start DNA Polymerase, nucleotides, and an optimized reaction buffer including MgCl 2
• Phusion U DNA Polymerase is a novel engineered high-fidelity enzyme developed using fusion technology
• Designed with a proprietary mutation in the dUTP binding pocket of Phusion polymerase, Phusion U polymerase is able to overcome important limitations of proofreading enzymes to incorporate dUTP and read through uracil present in DNA templates
• Phusion U polymerase features properties of Phusion DNA polymerases and offers the ability to amplify long amplicons up to 20 kb and a high specificity with affibody based hot start
• These features make Phusion U Hot Start DNA Polymerase an excellent choice for such applications as amplification of bisulfite-converted or damaged DNA as well as use of carryover contamination control
• Highlights High-fidelity DNA polymerase (25X Taq ) Engineered to incorporate dUTP and amplify uracil containing templates Hot start for reduced nonspecific amplification and primer degradation Only template and primers need to be added for use Short extension times (15–30 s/kb) Applications Amplification of bisulfite-converted DNA Amplification of damaged or aged DNA Carry-over contamination control Uracil-excision based (USER) cloning methods Using Phusion DNA Polymerases Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).