F533S
Thermo Scientific™ Phusion U Hot Start PCR Master Mix
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | F533S-Each-of-1 | In Stock | ₹ 31,999.44 |
F533S - Each of 1
In Stock
Quantity
1
Base Price: ₹ 31,999.44
GST (18%): ₹ 5,759.899
Total Price: ₹ 37,759.339
Content And Storage
• Phusion U Hot Start DNA Polymerase• 2X reaction buffer• 3 mM MgCl2• 400 μM each of dATP, dTTP, dCTP and dGTPMaster mix is supplied with nuclease-free water.Store at -20°C.
Polymerase
Phusion U Hot Start DNA Polymerase
Product Type
Hot Start PCR Master Mix
For Use With (Application)
Hot-start PCR
Fidelity (vs. Taq)
25X
No. of Reactions
100 Reactions
Reaction Format
SuperMix or Master Mix
GC-Rich PCR Performance
Medium
Reaction Speed
Fast
Shipping Condition
Dry Ice
Concentration
2X
Hot Start
Built-In Hot Start
Overhang
Blunt
Size (Final Product)
20 kb or less
Related Products
Description
- Thermo Scientific Phusion U Hot Start PCR Master Mix is a convenient 2X formula that contains Phusion U Hot Start DNA Polymerase, nucleotides, and an optimized reaction buffer including MgCl 2
- Phusion U DNA Polymerase is a novel engineered high-fidelity enzyme developed using fusion technology
- Designed with a proprietary mutation in the dUTP binding pocket of Phusion polymerase, Phusion U polymerase is able to overcome important limitations of proofreading enzymes to incorporate dUTP and read through uracil present in DNA templates
- Phusion U polymerase features properties of Phusion DNA polymerases and offers the ability to amplify long amplicons up to 20 kb and a high specificity with affibody based hot start
- These features make Phusion U Hot Start DNA Polymerase an excellent choice for such applications as amplification of bisulfite-converted or damaged DNA as well as use of carryover contamination control
- Highlights High-fidelity DNA polymerase (25X Taq ) Engineered to incorporate dUTP and amplify uracil containing templates Hot start for reduced nonspecific amplification and primer degradation Only template and primers need to be added for use Short extension times (15–30 s/kb) Applications Amplification of bisulfite-converted DNA Amplification of damaged or aged DNA Carry-over contamination control Uracil-excision based (USER) cloning methods Using Phusion DNA Polymerases Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).
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Content And Storage: • Phusion U Hot Start DNA Polymerase• 2X reaction buffer• 3 mM MgCl2• 400 μM each of dATP, dTTP, dCTP and dGTPMaster mix is supplied with nuclease-free water.Store at -20°C.
Polymerase: Phusion U Hot Start DNA Polymerase
Product Type: Hot Start PCR Master Mix
For Use With (Application): Hot-start PCR
Fidelity (vs. Taq): 25X
No. of Reactions: 100 Reactions
Reaction Format: SuperMix or Master Mix
GC-Rich PCR Performance: Medium
Reaction Speed: Fast
Shipping Condition: Dry Ice
Concentration: 2X
Hot Start: Built-In Hot Start
Overhang: Blunt
Size (Final Product): 20 kb or less
Content And Storage:
• Phusion U Hot Start DNA Polymerase• 2X reaction buffer• 3 mM MgCl2• 400 μM each of dATP, dTTP, dCTP and dGTPMaster mix is supplied with nuclease-free water.Store at -20°C.
Polymerase:
Phusion U Hot Start DNA Polymerase
Product Type:
Hot Start PCR Master Mix
For Use With (Application):
Hot-start PCR
Fidelity (vs. Taq):
25X
No. of Reactions:
100 Reactions
Reaction Format:
SuperMix or Master Mix
GC-Rich PCR Performance:
Medium
Reaction Speed:
Fast
Shipping Condition:
Dry Ice
Concentration:
2X
Hot Start:
Built-In Hot Start
Overhang:
Blunt
Size (Final Product):
20 kb or less
Content And Storage: • Phusion DNA Polymerase, 2 U/μL• 5X Phusion Green HF and GF Buffers• DMSO• 50 mM MgCl2Phusion Green HF and Phusion Green GC Buffers each provide 1.5 mM MgCl2in the final 1X concentration.Store at -20°C.
Polymerase: Phusion High-Fidelity DNA Polymerase
Product Type: High-Fidelity DNA Polymerase
For Use With (Application): Standard PCR, High-fidelity PCR
Fidelity (vs. Taq): 52X
No. of Reactions: 500 Reactions
Reaction Format: Standalone
GC-Rich PCR Performance: High
Reaction Speed: Fast
Shipping Condition: Dry Ice
Concentration: 2 U/μL
Hot Start: No
Overhang: Blunt
Size (Final Product): 20 kb or less
Content And Storage:
• Phusion DNA Polymerase, 2 U/μL• 5X Phusion Green HF and GF Buffers• DMSO• 50 mM MgCl2Phusion Green HF and Phusion Green GC Buffers each provide 1.5 mM MgCl2in the final 1X concentration.Store at -20°C.
Polymerase:
Phusion High-Fidelity DNA Polymerase
Product Type:
High-Fidelity DNA Polymerase
For Use With (Application):
Standard PCR, High-fidelity PCR
Fidelity (vs. Taq):
52X
No. of Reactions:
500 Reactions
Reaction Format:
Standalone
GC-Rich PCR Performance:
High
Reaction Speed:
Fast
Shipping Condition:
Dry Ice
Concentration:
2 U/μL
Hot Start:
No
Overhang:
Blunt
Size (Final Product):
20 kb or less
Content And Storage: • Phusion DNA Polymerase, 2 U/μL• 5X Phusion Green HF and GF Buffers• DMSO• 50 mM MgCl2Phusion Green HF and Phusion Green GC Buffers each provide 1.5 mM MgCl2in the final 1X concentration.Store at -20°C.
Polymerase: Phusion High-Fidelity DNA Polymerase
Product Type: High-Fidelity DNA Polymerase
For Use With (Application): Standard PCR, High-fidelity PCR
Fidelity (vs. Taq): 52X
No. of Reactions: 100 Reactions
Reaction Format: Standalone
GC-Rich PCR Performance: High
Reaction Speed: Fast
Shipping Condition: Dry Ice
Concentration: 2 U/μL
Hot Start: No
Overhang: Blunt
Size (Final Product): 20 kb or less
Content And Storage:
• Phusion DNA Polymerase, 2 U/μL• 5X Phusion Green HF and GF Buffers• DMSO• 50 mM MgCl2Phusion Green HF and Phusion Green GC Buffers each provide 1.5 mM MgCl2in the final 1X concentration.Store at -20°C.
Polymerase:
Phusion High-Fidelity DNA Polymerase
Product Type:
High-Fidelity DNA Polymerase
For Use With (Application):
Standard PCR, High-fidelity PCR
Fidelity (vs. Taq):
52X
No. of Reactions:
100 Reactions
Reaction Format:
Standalone
GC-Rich PCR Performance:
High
Reaction Speed:
Fast
Shipping Condition:
Dry Ice
Concentration:
2 U/μL
Hot Start:
No
Overhang:
Blunt
Size (Final Product):
20 kb or less
Content And Storage: • Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/μL)• 5X Phusion Green HF Buffer• 5X Phusion Green GC Buffer• DMSO• 50 mM MgCl2solutionPhusion Green HF and GC Buffers both provide 1.5 mM MgCl2in the final 1X concentration.Store at –20°C.
Polymerase: Phusion Hot Start II High-Fidelity DNA Polymerase
Product Type: Hot Start High-Fidelity DNA Polymerase
For Use With (Application): Hot-start PCR, High-fidelity PCR
Fidelity (vs. Taq): 52X
No. of Reactions: 500 Reactions
Reaction Format: Standalone
GC-Rich PCR Performance: High
Reaction Speed: Fast
Shipping Condition: Dry Ice
Concentration: 2 U/μL
Hot Start: Built-In Hot Start
Overhang: Blunt
Size (Final Product): 20 kb or less
Content And Storage:
• Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/μL)• 5X Phusion Green HF Buffer• 5X Phusion Green GC Buffer• DMSO• 50 mM MgCl2solutionPhusion Green HF and GC Buffers both provide 1.5 mM MgCl2in the final 1X concentration.Store at –20°C.
Polymerase:
Phusion Hot Start II High-Fidelity DNA Polymerase
Product Type:
Hot Start High-Fidelity DNA Polymerase
For Use With (Application):
Hot-start PCR, High-fidelity PCR
Fidelity (vs. Taq):
52X
No. of Reactions:
500 Reactions
Reaction Format:
Standalone
GC-Rich PCR Performance:
High
Reaction Speed:
Fast
Shipping Condition:
Dry Ice
Concentration:
2 U/μL
Hot Start:
Built-In Hot Start
Overhang:
Blunt
Size (Final Product):
20 kb or less