4927004
Applied Biosystems™ SYBR™ Green Universal Master Mix
Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient, single-tube format.
Manufacturer: Fischer Scientific
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Detection Method
SYBR
GC-Rich PCR Performance
High
Polymerase
AmpliTaq Gold DNA Polymerase
Label or Dye
SYBR Green
Product Line
SYBR™
For Use With (Application)
Gene Expression
Format
Tube
PCR Method
qPCR
Reaction Speed
Standard
Passive Reference Dye
ROX (Pre-mixed)
Product Type
Real Time PCR SYBR Master Mix
Concentration
2X
Related Products
Description
- Applied Biosystems™ SYBR™ Green Universal Master Mix combines SYBR™ Green I dye, AmpliTaq Gold™ DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial
- SYBR™ Green I dye detects double-stranded DNA, so specific probes are not required AmpliTaq Gold™ DNA polymerase minimizes nonspecific product formation to achieve superior performance dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase Proprietary buffer enhancements ensure performance and reliability Premixed components stored at 2° to 8°C significantly reduce assay setup time Maximum Flexibility and Convenience: Applied Biosystems™ SYBR™ Green Universal Master Mix provides maximum flexibility at reduced cost because no target-specific TaqMan™ probes are required SYBR™ Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR The hot-start enzyme AmpliTaq Gold™ DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity Passive Internal Reference 1 is provided to normalize non-PCR—related fluorescence fluctuations This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation SYBR™ Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed
- Store at -20°C Order Info Shipping Condition: Wet Ice
Compare Similar Items
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Detection Method: SYBR
GC-Rich PCR Performance: High
Polymerase: AmpliTaq Gold DNA Polymerase
Label or Dye: SYBR Green
Product Line: SYBR™
For Use With (Application): Gene Expression
Format: Tube
PCR Method: qPCR
Reaction Speed: Standard
Passive Reference Dye: ROX (Pre-mixed)
Product Type: Real Time PCR SYBR Master Mix
Concentration: 2X
Detection Method:
SYBR
GC-Rich PCR Performance:
High
Polymerase:
AmpliTaq Gold DNA Polymerase
Label or Dye:
SYBR Green
Product Line:
SYBR™
For Use With (Application):
Gene Expression
Format:
Tube
PCR Method:
qPCR
Reaction Speed:
Standard
Passive Reference Dye:
ROX (Pre-mixed)
Product Type:
Real Time PCR SYBR Master Mix
Concentration:
2X
Detection Method: SYBR
GC-Rich PCR Performance: High
Polymerase: AmpliTaq DNA Polymerase
Label or Dye: SYBR GreenER
Product Line: SYBR™
For Use With (Application): Gene Expression
Format: Vial
PCR Method: qPCR
Reaction Speed: Fast or Standard
Passive Reference Dye: ROX (Pre-mixed)
Product Type: Real Time PCR SYBR Master Mix
Concentration: 2X
Detection Method:
SYBR
GC-Rich PCR Performance:
High
Polymerase:
AmpliTaq DNA Polymerase
Label or Dye:
SYBR GreenER
Product Line:
SYBR™
For Use With (Application):
Gene Expression
Format:
Vial
PCR Method:
qPCR
Reaction Speed:
Fast or Standard
Passive Reference Dye:
ROX (Pre-mixed)
Product Type:
Real Time PCR SYBR Master Mix
Concentration:
2X
Detection Method: SYBR
GC-Rich PCR Performance: High
Polymerase: Taq DNA Polymerase
Label or Dye: __
Product Line: Platinum™, SYBR™
For Use With (Application): Gene Expression
Format: __
PCR Method: qPCR
Reaction Speed: Standard
Passive Reference Dye: __
Product Type: Real Time PCR SYBR Master Mix
Concentration: 2X
Detection Method:
SYBR
GC-Rich PCR Performance:
High
Polymerase:
Taq DNA Polymerase
Label or Dye:
__
Product Line:
Platinum™, SYBR™
For Use With (Application):
Gene Expression
Format:
__
PCR Method:
qPCR
Reaction Speed:
Standard
Passive Reference Dye:
__
Product Type:
Real Time PCR SYBR Master Mix
Concentration:
2X
Detection Method: SYBR
GC-Rich PCR Performance: High
Polymerase: Taq DNA Polymerase
Label or Dye: __
Product Line: Platinum™, SYBR™ GreenER™
For Use With (Application): Gene Expression
Format: __
PCR Method: qPCR
Reaction Speed: Standard
Passive Reference Dye: __
Product Type: Real Time PCR SYBR Master Mix
Concentration: 2X
Detection Method:
SYBR
GC-Rich PCR Performance:
High
Polymerase:
Taq DNA Polymerase
Label or Dye:
__
Product Line:
Platinum™, SYBR™ GreenER™
For Use With (Application):
Gene Expression
Format:
__
PCR Method:
qPCR
Reaction Speed:
Standard
Passive Reference Dye:
__
Product Type:
Real Time PCR SYBR Master Mix
Concentration:
2X