7087576

Cytiva Thermo Sequenase DNA Polymerase

Novel thermostable DNA polymerase that uses dideoxynucleotide triphosphates (ddNTPs) as readily as deoxynucleotide (dNTP) substrates

Manufacturer: Fischer Scientific

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Content And Storage

-20°C, Solution in 20 mM Tris-HCl, pH 8.5, 0.1 mM EDTA, 0.5% Tween 20 (v/v), 0.5% Nonidet P-40 (v/v), 1 mM DTT, 100 mM KCl, 50% glycerol

For Use With (Application)

Sequencing, Genotyping

Source

E. coli clones that overproduce the protein

Description

Thermo Sequenase DNA Polymerase

No. of Reactions

32 units/μL

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Description

  • Uniform and easy-to-read band patterns Better incorporation of ddNTPs helps avoid secondary structures that can cause unreadable sequences Read-through of difficult sequences (e.g., GC-rich), due to the ability of thermostable enzymes to function at elevated temperature used for sequencing ddNTPs are chain-elongating inhibitors of DNA polymerase, which are used in the Sanger method for DNA sequencing Each nucleotide base of this particular type has a probability of being bonded not to a deoxynucleotide but rather a dideoxynucleotide, which ends chain elongation An excellent choice for cycle sequencing where template quantity limitations preclude the use of T7 Sequenase DNA polymerase Can also be used in many SNP genotyping, genotyping by sequencing, or genome sequencing applications where primer extension is employed

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-20°C, Solution in 20 mM Tris-HCl, pH 8.5, 0.1 mM EDTA, 0.5% Tween 20 (v/v), 0.5% Nonidet P-40 (v/v), 1 mM DTT, 100 mM KCl, 50% glycerol

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