A59528
Fisherbrand™ SYBR™ Green qPCR Master Mix
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | A59528-Each-of-1 | In Stock | ₹ 5,261.94 |
A59528 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 5,261.94
GST (18%): ₹ 947.149
Total Price: ₹ 6,209.089
Content And Storage
SYBR Green dye, DNA polymerase, with an antibody-mediated hot start, Heat-labile uracil-DNA glycosylase (UDG), ROX dye (passive reference dye), dNTP blend containing dUTP/dTTP, Optimized buffer components; Store at -20°C ± 10°C in the dark
Form
Frozen
PCR Method
qPCR, 2-step RT-qPCR, RT-qPCR
Label or Dye
SYBR Green, FAM
Shipping Condition
Dry Ice
Concentration
2X
Volume
1 mL
Detection Method
SYBR
GC-Rich PCR Performance
High
Reaction Speed
Fast or Standard
Product Type
Master Mix
For Use With (Application)
Gene Expression, DNA Quantitation, Microbial Detection, Real Time PCR (qPCR)
No. of Reactions
100 x 20 μL Reactions
Related Products
Description
- Features of Fisherbrand SYBR Green qPCR Master Mix include: Broad instrument and workflow compatibility enabling Fisherbrand SYBR Green qPCR Master Mix to be used with a wide variety of real-time PCR instruments and reverse transcriptase (RT) enzymes Highly sensitive and specific low-level detection of your target of interest Wide linear dynamic range —precise measurement across six logs of dynamic range, enabling accurate detection with both low- and high -concentration samples 1 Broad instrument compatibility Fisherbrand SYBR Green qPCR Master Mix can be used in either standard or fast cycling mode and is compatible with a wide variety of real-time PCR instruments
- Broad workflow compatibility Fisherbrand SYBR Green qPCR Master Mix is compatible with a variety of RT enzymes and provides flexibility for varying primer melting temperatures (Tm), between 55°C and 65°C
- Highly sensitive and specific Fisherbrand SYBR Green qPCR Master Mix enables low-level detection and contains an antibody-mediated hot-start mechanism to mitigate non-specific early polymerase activity
- See figure for a performance comparison vs
- other leading master mixes
- Wide dynamic range Fisherbrand SYBR Green qPCR Master Mix can yield up to 6-log dynamic range for cDNA (see figure) and shows equivalent or better dynamic range in the detection of various targets compared to other master mixes
- Other features include: Stability of pre-assembled reactions for up to 72 hours in dark room at room temperature 2 Reduce carryover contamination—uracil-DNA glycosylase (UDG) and dUTP included in mix to help ensure leftover DNA residue is not re-amplified in subsequent experiments Footnotes 1
- See user guide for specific sample concentration recommendations
- 2
- Pre-PCR stability is influenced not only by the master mix, but also by the target being analyzed
- For maximum confidence, researchers should confirm stability profiles of their specific targets
- For Research Use Only
- Not for use in diagnostic procedures.
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Content And Storage: SYBR Green dye, DNA polymerase, with an antibody-mediated hot start, Heat-labile uracil-DNA glycosylase (UDG), ROX dye (passive reference dye), dNTP blend containing dUTP/dTTP, Optimized buffer components; Store at -20°C ± 10°C in the dark
Form: Frozen
PCR Method: qPCR, 2-step RT-qPCR, RT-qPCR
Label or Dye: SYBR Green, FAM
Shipping Condition: Dry Ice
Concentration: 2X
Volume: 1 mL
Detection Method: SYBR
GC-Rich PCR Performance: High
Reaction Speed: Fast or Standard
Product Type: Master Mix
For Use With (Application): Gene Expression, DNA Quantitation, Microbial Detection, Real Time PCR (qPCR)
No. of Reactions: 100 x 20 μL Reactions
Content And Storage:
SYBR Green dye, DNA polymerase, with an antibody-mediated hot start, Heat-labile uracil-DNA glycosylase (UDG), ROX dye (passive reference dye), dNTP blend containing dUTP/dTTP, Optimized buffer components; Store at -20°C ± 10°C in the dark
Form:
Frozen
PCR Method:
qPCR, 2-step RT-qPCR, RT-qPCR
Label or Dye:
SYBR Green, FAM
Shipping Condition:
Dry Ice
Concentration:
2X
Volume:
1 mL
Detection Method:
SYBR
GC-Rich PCR Performance:
High
Reaction Speed:
Fast or Standard
Product Type:
Master Mix
For Use With (Application):
Gene Expression, DNA Quantitation, Microbial Detection, Real Time PCR (qPCR)
No. of Reactions:
100 x 20 μL Reactions
Content And Storage: SYBR Green dye, DNA polymerase, with an antibody-mediated hot start, Heat-labile uracil-DNA glycosylase (UDG), ROX dye (passive reference dye), dNTP blend containing dUTP/dTTP, Optimized buffer components; Store at -20°C ± 10°C in the dark
Form: Frozen
PCR Method: qPCR, 2-step RT-qPCR, RT-qPCR
Label or Dye: SYBR Green, FAM
Shipping Condition: Dry Ice
Concentration: 2X
Volume: 5 mL
Detection Method: SYBR
GC-Rich PCR Performance: High
Reaction Speed: Fast or Standard
Product Type: Master Mix
For Use With (Application): Gene Expression, DNA Quantitation, Microbial Detection, Real Time PCR (qPCR)
No. of Reactions: 500 x 20 μL Reactions
Content And Storage:
SYBR Green dye, DNA polymerase, with an antibody-mediated hot start, Heat-labile uracil-DNA glycosylase (UDG), ROX dye (passive reference dye), dNTP blend containing dUTP/dTTP, Optimized buffer components; Store at -20°C ± 10°C in the dark
Form:
Frozen
PCR Method:
qPCR, 2-step RT-qPCR, RT-qPCR
Label or Dye:
SYBR Green, FAM
Shipping Condition:
Dry Ice
Concentration:
2X
Volume:
5 mL
Detection Method:
SYBR
GC-Rich PCR Performance:
High
Reaction Speed:
Fast or Standard
Product Type:
Master Mix
For Use With (Application):
Gene Expression, DNA Quantitation, Microbial Detection, Real Time PCR (qPCR)
No. of Reactions:
500 x 20 μL Reactions
Content And Storage: SYBR Green dye, DNA polymerase, with an antibody-mediated hot start, Heat-labile uracil-DNA glycosylase (UDG), ROX dye (passive reference dye), dNTP blend containing dUTP/dTTP, Optimized buffer components; Store at -20°C ± 10°C in the dark
Form: Frozen
PCR Method: qPCR, 2-step RT-qPCR, RT-qPCR
Label or Dye: SYBR Green, FAM
Shipping Condition: Dry Ice
Concentration: 2X
Volume: 50 mL
Detection Method: SYBR
GC-Rich PCR Performance: High
Reaction Speed: Fast or Standard
Product Type: Master Mix
For Use With (Application): Gene Expression, DNA Quantitation, Microbial Detection, Real Time PCR (qPCR)
No. of Reactions: 5000 x 20 μL Reactions
Content And Storage:
SYBR Green dye, DNA polymerase, with an antibody-mediated hot start, Heat-labile uracil-DNA glycosylase (UDG), ROX dye (passive reference dye), dNTP blend containing dUTP/dTTP, Optimized buffer components; Store at -20°C ± 10°C in the dark
Form:
Frozen
PCR Method:
qPCR, 2-step RT-qPCR, RT-qPCR
Label or Dye:
SYBR Green, FAM
Shipping Condition:
Dry Ice
Concentration:
2X
Volume:
50 mL
Detection Method:
SYBR
GC-Rich PCR Performance:
High
Reaction Speed:
Fast or Standard
Product Type:
Master Mix
For Use With (Application):
Gene Expression, DNA Quantitation, Microbial Detection, Real Time PCR (qPCR)
No. of Reactions:
5000 x 20 μL Reactions
Content And Storage: __
Form: __
PCR Method: __
Label or Dye: __
Shipping Condition: __
Concentration: __
Volume: __
Detection Method: __
GC-Rich PCR Performance: __
Reaction Speed: __
Product Type: __
For Use With (Application): __
No. of Reactions: __
Content And Storage:
__
Form:
__
PCR Method:
__
Label or Dye:
__
Shipping Condition:
__
Concentration:
__
Volume:
__
Detection Method:
__
GC-Rich PCR Performance:
__
Reaction Speed:
__
Product Type:
__
For Use With (Application):
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No. of Reactions:
__