200006732
Fisherbrand™ SYBR™ Green qPCR Master Mix
Fisherbrand™ SYBR™ Green qPCR Master Mix is a 2X, ready-to-use master mix designed for dye-based, quantitative amplification of DNA (cDNA and gDNA) targets by real-time PCR.
Manufacturer: Fischer Scientific
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Content And Storage
SYBR Green dye, DNA polymerase, with an antibody-mediated hot start, Heat-labile uracil-DNA glycosylase (UDG), ROX dye (passive reference dye), dNTP blend containing dUTP/dTTP, Optimized buffer components; Store at -20°C ± 10°C in the dark
Form
Frozen
PCR Method
qPCR, 2-step RT-qPCR, RT-qPCR
Label or Dye
SYBR Green, FAM
Shipping Condition
Dry Ice
Concentration
2X
Detection Method
SYBR
GC-Rich PCR Performance
High
Reaction Speed
Fast or Standard
Product Type
Master Mix
For Use With (Application)
Gene Expression, DNA Quantitation, Microbial Detection, Real Time PCR (qPCR)
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Description
- Features of Fisherbrand SYBR Green qPCR Master Mix include: Broad instrument and workflow compatibility enabling Fisherbrand SYBR Green qPCR Master Mix to be used with a wide variety of real-time PCR instruments and reverse transcriptase (RT) enzymes Highly sensitive and specific low-level detection of your target of interest Wide linear dynamic range —precise measurement across six logs of dynamic range, enabling accurate detection with both low- and high -concentration samples 1 Broad instrument compatibility Fisherbrand SYBR Green qPCR Master Mix can be used in either standard or fast cycling mode and is compatible with a wide variety of real-time PCR instruments
- Broad workflow compatibility Fisherbrand SYBR Green qPCR Master Mix is compatible with a variety of RT enzymes and provides flexibility for varying primer melting temperatures (Tm), between 55°C and 65°C
- Highly sensitive and specific Fisherbrand SYBR Green qPCR Master Mix enables low-level detection and contains an antibody-mediated hot-start mechanism to mitigate non-specific early polymerase activity
- See figure for a performance comparison vs
- other leading master mixes
- Wide dynamic range Fisherbrand SYBR Green qPCR Master Mix can yield up to 6-log dynamic range for cDNA (see figure) and shows equivalent or better dynamic range in the detection of various targets compared to other master mixes
- Other features include: Stability of pre-assembled reactions for up to 72 hours in dark room at room temperature 2 Reduce carryover contamination—uracil-DNA glycosylase (UDG) and dUTP included in mix to help ensure leftover DNA residue is not re-amplified in subsequent experiments Footnotes 1
- See user guide for specific sample concentration recommendations
- 2
- Pre-PCR stability is influenced not only by the master mix, but also by the target being analyzed
- For maximum confidence, researchers should confirm stability profiles of their specific targets
- For Research Use Only
- Not for use in diagnostic procedures.
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Content And Storage: SYBR Green dye, DNA polymerase, with an antibody-mediated hot start, Heat-labile uracil-DNA glycosylase (UDG), ROX dye (passive reference dye), dNTP blend containing dUTP/dTTP, Optimized buffer components; Store at -20°C ± 10°C in the dark
Form: Frozen
PCR Method: qPCR, 2-step RT-qPCR, RT-qPCR
Label or Dye: SYBR Green, FAM
Shipping Condition: Dry Ice
Concentration: 2X
Detection Method: SYBR
GC-Rich PCR Performance: High
Reaction Speed: Fast or Standard
Product Type: Master Mix
For Use With (Application): Gene Expression, DNA Quantitation, Microbial Detection, Real Time PCR (qPCR)
Content And Storage:
SYBR Green dye, DNA polymerase, with an antibody-mediated hot start, Heat-labile uracil-DNA glycosylase (UDG), ROX dye (passive reference dye), dNTP blend containing dUTP/dTTP, Optimized buffer components; Store at -20°C ± 10°C in the dark
Form:
Frozen
PCR Method:
qPCR, 2-step RT-qPCR, RT-qPCR
Label or Dye:
SYBR Green, FAM
Shipping Condition:
Dry Ice
Concentration:
2X
Detection Method:
SYBR
GC-Rich PCR Performance:
High
Reaction Speed:
Fast or Standard
Product Type:
Master Mix
For Use With (Application):
Gene Expression, DNA Quantitation, Microbial Detection, Real Time PCR (qPCR)
Content And Storage: Store in freezer (-5 to -30°C). Protect from light.
Form: __
PCR Method: __
Label or Dye: __
Shipping Condition: __
Concentration: __
Detection Method: __
GC-Rich PCR Performance: __
Reaction Speed: __
Product Type: Dye
For Use With (Application): __
Content And Storage:
Store in freezer (-5 to -30°C). Protect from light.
Form:
__
PCR Method:
__
Label or Dye:
__
Shipping Condition:
__
Concentration:
__
Detection Method:
__
GC-Rich PCR Performance:
__
Reaction Speed:
__
Product Type:
Dye
For Use With (Application):
__
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Form: Liquid
PCR Method: __
Label or Dye: __
Shipping Condition: __
Concentration: 5 μL/Test
Detection Method: __
GC-Rich PCR Performance: __
Reaction Speed: __
Product Type: __
For Use With (Application): __
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Form:
Liquid
PCR Method:
__
Label or Dye:
__
Shipping Condition:
__
Concentration:
5 μL/Test
Detection Method:
__
GC-Rich PCR Performance:
__
Reaction Speed:
__
Product Type:
__
For Use With (Application):
__
Content And Storage: 4° C, store in dark, DO NOT FREEZE!
Form: Liquid
PCR Method: __
Label or Dye: __
Shipping Condition: __
Concentration: 0.2 mg/mL
Detection Method: __
GC-Rich PCR Performance: __
Reaction Speed: __
Product Type: __
For Use With (Application): __
Content And Storage:
4° C, store in dark, DO NOT FREEZE!
Form:
Liquid
PCR Method:
__
Label or Dye:
__
Shipping Condition:
__
Concentration:
0.2 mg/mL
Detection Method:
__
GC-Rich PCR Performance:
__
Reaction Speed:
__
Product Type:
__
For Use With (Application):
__