F547S
Thermo Scientific™ Phusion Blood Direct PCR Kit
Manufacturer: Fischer Scientific
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | F547S-Each-of-1 | In Stock | ₹ 15,015.78 |
F547S - Each of 1
In Stock
Quantity
1
Base Price: ₹ 15,015.78
GST (18%): ₹ 2,702.84
Total Price: ₹ 17,718.62
Content And Storage
• Phusion Blood II DNA Polymerase, 40 μL• 2X Phusion Blood PCR Buffer with dNTPs and MgCl2, 1.25 mL• Universal Control Primer Mix, 40 μL• 50 mM EDTA, 0.5 mL• 50 mM MgCl2, 1.5 mL• DMSO, 0.5 mLStore at -20°C.
PCR Method
Direct PCR
Reaction Speed
Fast
Shipping Condition
Wet or Dry Ice
Hot Start
Built-In Hot Start
Overhang
Blunt
Sample Type
Blood
GC-Rich PCR Performance
High
Polymerase
Phusion Blood II DNA Polymerase
Product Type
Direct PCR Kit
Concentration
2 U/μL
No. of Reactions
100 Reactions
Reaction Format
Separate Components
Starting Material
DNA
Related Products
Description
- Thermo Scientific Phusion Blood Direct PCR Kit is for use in the amplification of DNA from whole blood
- It eliminates the need for a separate DNA purification step prior to PCR
- The modified Phusion Hot Start II High-Fidelity DNA Polymerase exhibits high resistance to PCR inhibitors found in blood and retains polymerase activity even with samples containing 40% whole blood
- This DNA polymerase exhibits exceptional accuracy in proofreading and makes the Phusion Blood Direct PCR Kit one of the best choices for demanding applications, such as cloning
- The Phusion Blood Direct PCR Kit is versatile and permits analysis of blood samples from various species and with different anticoagulants or storage conditions
- Features No need for time-consuming and expensive DNA purification steps Simple protocol requires minimal hands-on time Extremely short PCR protocol times Modified Phusion Hot Start II High-Fidelity DNA Polymerase delivers abundant yields of specific product Notes The optimal annealing temperature for Phusion DNA Polymerases may differ significantly from that of Taq -based polymerases.
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Content And Storage: • Phusion Blood II DNA Polymerase, 40 μL• 2X Phusion Blood PCR Buffer with dNTPs and MgCl2, 1.25 mL• Universal Control Primer Mix, 40 μL• 50 mM EDTA, 0.5 mL• 50 mM MgCl2, 1.5 mL• DMSO, 0.5 mLStore at -20°C.
PCR Method: Direct PCR
Reaction Speed: Fast
Shipping Condition: Wet or Dry Ice
Hot Start: Built-In Hot Start
Overhang: Blunt
Sample Type: Blood
GC-Rich PCR Performance: High
Polymerase: Phusion Blood II DNA Polymerase
Product Type: Direct PCR Kit
Concentration: 2 U/μL
No. of Reactions: 100 Reactions
Reaction Format: Separate Components
Starting Material: DNA
Content And Storage:
• Phusion Blood II DNA Polymerase, 40 μL• 2X Phusion Blood PCR Buffer with dNTPs and MgCl2, 1.25 mL• Universal Control Primer Mix, 40 μL• 50 mM EDTA, 0.5 mL• 50 mM MgCl2, 1.5 mL• DMSO, 0.5 mLStore at -20°C.
PCR Method:
Direct PCR
Reaction Speed:
Fast
Shipping Condition:
Wet or Dry Ice
Hot Start:
Built-In Hot Start
Overhang:
Blunt
Sample Type:
Blood
GC-Rich PCR Performance:
High
Polymerase:
Phusion Blood II DNA Polymerase
Product Type:
Direct PCR Kit
Concentration:
2 U/μL
No. of Reactions:
100 Reactions
Reaction Format:
Separate Components
Starting Material:
DNA
Content And Storage: Phusion Flash High-Fidelity PCR Master Mix (5 x 1 mL)Store at -20°C.
PCR Method: __
Reaction Speed: Fast
Shipping Condition: Dry Ice
Hot Start: Built-In Hot Start
Overhang: Blunt
Sample Type: __
GC-Rich PCR Performance: High
Polymerase: Phusion Flash II DNA Polymerase
Product Type: High-Fidelity PCR Master Mix
Concentration: 2X
No. of Reactions: 500 Reactions
Reaction Format: SuperMix or Master Mix
Starting Material: __
Content And Storage:
Phusion Flash High-Fidelity PCR Master Mix (5 x 1 mL)Store at -20°C.
PCR Method:
__
Reaction Speed:
Fast
Shipping Condition:
Dry Ice
Hot Start:
Built-In Hot Start
Overhang:
Blunt
Sample Type:
__
GC-Rich PCR Performance:
High
Polymerase:
Phusion Flash II DNA Polymerase
Product Type:
High-Fidelity PCR Master Mix
Concentration:
2X
No. of Reactions:
500 Reactions
Reaction Format:
SuperMix or Master Mix
Starting Material:
__
Content And Storage: Phusion Flash High-Fidelity PCR Master Mix (1 mL)Store at -20°C.
PCR Method: __
Reaction Speed: Fast
Shipping Condition: Dry Ice
Hot Start: Built-In Hot Start
Overhang: Blunt
Sample Type: __
GC-Rich PCR Performance: High
Polymerase: Phusion Flash II DNA Polymerase
Product Type: High-Fidelity PCR Master Mix
Concentration: 2X
No. of Reactions: 100 Reactions
Reaction Format: SuperMix or Master Mix
Starting Material: __
Content And Storage:
Phusion Flash High-Fidelity PCR Master Mix (1 mL)Store at -20°C.
PCR Method:
__
Reaction Speed:
Fast
Shipping Condition:
Dry Ice
Hot Start:
Built-In Hot Start
Overhang:
Blunt
Sample Type:
__
GC-Rich PCR Performance:
High
Polymerase:
Phusion Flash II DNA Polymerase
Product Type:
High-Fidelity PCR Master Mix
Concentration:
2X
No. of Reactions:
100 Reactions
Reaction Format:
SuperMix or Master Mix
Starting Material:
__
Content And Storage: • Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/μL)• 5X Phusion HF & GC Buffers• DMSO• 50 mM MgCl2solutionPhusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2in the final 1X concentration.Store at -20°C.
PCR Method: __
Reaction Speed: Fast
Shipping Condition: Dry Ice
Hot Start: Built-In Hot Start
Overhang: Blunt
Sample Type: __
GC-Rich PCR Performance: High
Polymerase: Phusion Hot Start II DNA Polymerase
Product Type: Hot Start DNA Polymerase
Concentration: 2 U/μL
No. of Reactions: 500 Reactions
Reaction Format: Standalone
Starting Material: __
Content And Storage:
• Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/μL)• 5X Phusion HF & GC Buffers• DMSO• 50 mM MgCl2solutionPhusion HF Buffer and Phusion GC Buffers each provide 1.5 mM MgCl2in the final 1X concentration.Store at -20°C.
PCR Method:
__
Reaction Speed:
Fast
Shipping Condition:
Dry Ice
Hot Start:
Built-In Hot Start
Overhang:
Blunt
Sample Type:
__
GC-Rich PCR Performance:
High
Polymerase:
Phusion Hot Start II DNA Polymerase
Product Type:
Hot Start DNA Polymerase
Concentration:
2 U/μL
No. of Reactions:
500 Reactions
Reaction Format:
Standalone
Starting Material:
__