Thermo Scientific™ RevertAid RT Reverse Transcription Kit

Description

• The Thermo Scientific RevertAid RT Kit is a complete system for efficient synthesis of first strand cDNA from mRNA or total RNA templates
• The kit uses RevertAid Reverse Transcriptase (RT), which has lower RNase H activity compared to AMV reverse transcriptase
• The enzyme maintains activity at 42–50°C and is suitable for synthesis of cDNA up to 13 kb
• The recombinant RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA from degradation at temperatures up to 55°C
• First strand cDNA synthesized with this system can be directly used as a template in PCR or real-time PCR
• It is also ideal as a first step for second strand cDNA synthesis or linear RNA amplification
• Radioactively and non-radioactively labeled nucleotides can be incorporated into first strand cDNA for use as a probe in hybridization experiments, including microarrays
• Features of the RevertAid RT Kit include: • Full-length first strand cDNA up to 13 kb • Optimum reaction temperature 42°C • Complete kit includes all the components for RT reactions Applications • First strand cDNA synthesis for RT-PCR and RT-qPCR • Construction of full length cDNA libraries • Antisense RNA synthesis Order Info Orders placed Monday through Wednesday ship next day; Thursday and Friday orders ship the following Monday.