11-904-018
Invitrogen™ SuperScript™ First-Strand Synthesis System for RT-PCR
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 11-904-018-Each-of-1 | In Stock | ₹ 48,940.32 |
11-904-018 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 48,940.32
GST (18%): ₹ 8,809.258
Total Price: ₹ 57,749.578
Content And Storage
• Oligo(dT)12-18, 50 μL (0.5 μg/μL)• Random hexamers, 250 μL (50 ng/μL)• 10X RT buffer, 1 mL• Magnesium chloride, 500 μL (25 mM)• DTT, 250 μL (0.1 M)• dNTP mix, 250 μL (10 mM)• SuperScript™ II RT, 50 μL (50 U/μL)• RNaseOUT™, 100 μL (40 U/μL)•E. coliRNase H, 50 μL (2 U/μL)• DEPC-treated water, 1.2 mL• Control RNA, 15 μL (50 ng/μL)• Control Primer A, 20 μL (10 μM)• Control Primer B, 20 μL (10 μM)Store at –20°C.
GC-Rich PCR Performance
High
Technique
Reverse Transcription
Primer
Random Primers, Oligo dT Primers
Shipping Condition
Wet Ice
Final Product Type
First-Strand cDNA
Reaction Format
Separate components
Size (Final Product)
Up to 12 kb
Format
Kit
Reaction Speed
50 min.
Optimal Reaction Temperature
42°C
Reverse Transcriptase
SuperScript II
For Use With (Application)
RT-PCR
No. of Reactions
50 Reactions
Reagent Type
Reverse Transcription
Starting Material
RNA
Related Products
Description
- The SuperScript First-Strand Synthesis System for RT-PCR is optimized for the synthesis of first-strand cDNA from purified poly(A) + or total RNA
- The system can be used with as little as 1 ng or as much as 5 μg of total RNA
- After synthesis, the cDNA can be amplified with specific primers by PCR without intermediate organic extractions or ethanol precipitations
- In conjunction with PCR, the system can be used to detect the presence of rare messages, to quantitate the amount of specific mRNA from small numbers of cells, or to clone specific cDNAs without constructing an entire cDNA library
- The system is flexible, allowing the use of any PCR enzyme
- Combine with AccuPrime Taq DNA Polymerase or Platinum Taq DNA Polymerase for higher specificity PCR or with AccuPrime Pfx DNA Polymerase for high-fidelity cloning applications
- SuperScript II RT The first-strand cDNA synthesis reaction is catalyzed by SuperScript II Reverse Transcriptase (RT)
- This enzyme has been engineered to reduce the RNase H activity that degrades mRNA during the first-strand reaction, resulting in greater full-length cDNA synthesis and higher yields of first-strand cDNA than obtained with RNase H+ RTs
- Because SuperScript II RT is not inhibited significantly by ribosomal and transfer RNA, it may be used effectively to synthesize first-strand cDNA from a total RNA preparation
- The enzyme exhibits increased thermal stability and may be used at temperatures up to 50°C
- Using the SuperScript First-Strand Synthesis System for RT-PCR This system has been optimized to synthesize first-strand cDNA from varying amounts of starting material
- The SuperScript II RT concentration has been lowered and RNaseOUT Recombinant RNase Inhibitor has been added to the system as part of this optimization process
- Additionally, reaction conditions have been modified to further increase the sensitivity of the system
- Using the kit, you synthesize first-strand cDNA using either total RNA or poly(A) + selected RNA primed with oligo(dT), random primers, or a gene-specific primer
- Then perform PCR in a separate tube using primers specific for the gene of interest
- Order Info Shipping Condition: Wet ice
Compare Similar Items
Show Difference
Content And Storage: • Oligo(dT)12-18, 50 μL (0.5 μg/μL)• Random hexamers, 250 μL (50 ng/μL)• 10X RT buffer, 1 mL• Magnesium chloride, 500 μL (25 mM)• DTT, 250 μL (0.1 M)• dNTP mix, 250 μL (10 mM)• SuperScript™ II RT, 50 μL (50 U/μL)• RNaseOUT™, 100 μL (40 U/μL)•E. coliRNase H, 50 μL (2 U/μL)• DEPC-treated water, 1.2 mL• Control RNA, 15 μL (50 ng/μL)• Control Primer A, 20 μL (10 μM)• Control Primer B, 20 μL (10 μM)Store at –20°C.
GC-Rich PCR Performance: High
Technique: Reverse Transcription
Primer: Random Primers, Oligo dT Primers
Shipping Condition: Wet Ice
Final Product Type: First-Strand cDNA
Reaction Format: Separate components
Size (Final Product): Up to 12 kb
Format: Kit
Reaction Speed: 50 min.
Optimal Reaction Temperature: 42°C
Reverse Transcriptase: SuperScript II
For Use With (Application): RT-PCR
No. of Reactions: 50 Reactions
Reagent Type: Reverse Transcription
Starting Material: RNA
Content And Storage:
• Oligo(dT)12-18, 50 μL (0.5 μg/μL)• Random hexamers, 250 μL (50 ng/μL)• 10X RT buffer, 1 mL• Magnesium chloride, 500 μL (25 mM)• DTT, 250 μL (0.1 M)• dNTP mix, 250 μL (10 mM)• SuperScript™ II RT, 50 μL (50 U/μL)• RNaseOUT™, 100 μL (40 U/μL)•E. coliRNase H, 50 μL (2 U/μL)• DEPC-treated water, 1.2 mL• Control RNA, 15 μL (50 ng/μL)• Control Primer A, 20 μL (10 μM)• Control Primer B, 20 μL (10 μM)Store at –20°C.
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription
Primer:
Random Primers, Oligo dT Primers
Shipping Condition:
Wet Ice
Final Product Type:
First-Strand cDNA
Reaction Format:
Separate components
Size (Final Product):
Up to 12 kb
Format:
Kit
Reaction Speed:
50 min.
Optimal Reaction Temperature:
42°C
Reverse Transcriptase:
SuperScript II
For Use With (Application):
RT-PCR
No. of Reactions:
50 Reactions
Reagent Type:
Reverse Transcription
Starting Material:
RNA
Content And Storage: __
GC-Rich PCR Performance: __
Technique: __
Primer: __
Shipping Condition: __
Final Product Type: __
Reaction Format: __
Size (Final Product): __
Format: __
Reaction Speed: __
Optimal Reaction Temperature: __
Reverse Transcriptase: __
For Use With (Application): __
No. of Reactions: __
Reagent Type: __
Starting Material: __
Content And Storage:
__
GC-Rich PCR Performance:
__
Technique:
__
Primer:
__
Shipping Condition:
__
Final Product Type:
__
Reaction Format:
__
Size (Final Product):
__
Format:
__
Reaction Speed:
__
Optimal Reaction Temperature:
__
Reverse Transcriptase:
__
For Use With (Application):
__
No. of Reactions:
__
Reagent Type:
__
Starting Material:
__
Content And Storage: __
GC-Rich PCR Performance: __
Technique: __
Primer: __
Shipping Condition: __
Final Product Type: __
Reaction Format: __
Size (Final Product): __
Format: __
Reaction Speed: __
Optimal Reaction Temperature: __
Reverse Transcriptase: __
For Use With (Application): For writing, copying and printing and cleanroom documentation
No. of Reactions: __
Reagent Type: __
Starting Material: __
Content And Storage:
__
GC-Rich PCR Performance:
__
Technique:
__
Primer:
__
Shipping Condition:
__
Final Product Type:
__
Reaction Format:
__
Size (Final Product):
__
Format:
__
Reaction Speed:
__
Optimal Reaction Temperature:
__
Reverse Transcriptase:
__
For Use With (Application):
For writing, copying and printing and cleanroom documentation
No. of Reactions:
__
Reagent Type:
__
Starting Material:
__
Content And Storage: __
GC-Rich PCR Performance: __
Technique: __
Primer: __
Shipping Condition: __
Final Product Type: __
Reaction Format: __
Size (Final Product): __
Format: __
Reaction Speed: __
Optimal Reaction Temperature: __
Reverse Transcriptase: __
For Use With (Application): __
No. of Reactions: __
Reagent Type: __
Starting Material: __
Content And Storage:
__
GC-Rich PCR Performance:
__
Technique:
__
Primer:
__
Shipping Condition:
__
Final Product Type:
__
Reaction Format:
__
Size (Final Product):
__
Format:
__
Reaction Speed:
__
Optimal Reaction Temperature:
__
Reverse Transcriptase:
__
For Use With (Application):
__
No. of Reactions:
__
Reagent Type:
__
Starting Material:
__