18-080-400
Invitrogen™ SuperScript™ III First-Strand Synthesis SuperMix
Manufacturer: Invitrogen™
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | 18-080-400-Each-of-1 | In Stock | ₹ 57,672.00 |
18-080-400 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 57,672.00
GST (18%): ₹ 10,380.96
Total Price: ₹ 68,052.96
Content And Storage
• 2X Reaction Mix, 500 μL• SuperScript™ III Enzyme Mix, 100 μL• Oligo(dT)20, 50 μL total (50 μM)• Random hexamers, 50 μL (50 ng/μL)• Annealing buffer, 50 μLStore at –20°C.
GC-Rich PCR Performance
High
Technique
Reverse Transcription
Primer
Random Primers, Oligo dT Primers
Shipping Condition
Dry Ice
Final Product Type
First-Strand cDNA
Reaction Format
Master Mix
Starting Material
RNA
Format
Kit
Reaction Speed
50 min.
Optimal Reaction Temperature
50°C
Reverse Transcriptase
SuperScript III
For Use With (Application)
Real Time PCR (qPCR)
No. of Reactions
50 Reactions
Reagent Type
Reverse Transcription
Related Products
Description
- The SuperScript III First-Strand Synthesis SuperMix is an optimized formulation for first-strand cDNA synthesis from purified poly(A) + or total RNA
- RNA targets from 100 bp to >12 kb can be detected with this system
- The amount of starting material can vary from 0.1 pg to 5 μg of total RNA
- This kit includes SuperScript III/RNaseOUT enzyme mix, 2X First-Strand reaction mix, and an annealing buffer
- SuperScript III Reverse Transcriptase is a version of MMLV RT that has been engineered to reduce RNase H activity and provide increased thermal stability
- The enzyme can be used to synthesize cDNA at a temperature range of 45–55°C, providing increased specificity, higher yields of cDNA, and more full-length product than many previous reverse transcriptases
- Using SuperScript III First-Strand Synthesis SuperMix SuperScript III RT is not significantly inhibited by ribosomal and transfer RNA and can be used to synthesize cDNA from total RNA
- RNaseOUT Recombinant RNase Inhibitor is included in the enzyme mix to safeguard against degradation of target RNA due to ribonuclease contamination
- The 2X First-Strand reaction mix includes 10 mM MgCl 2 and 1 mM of each dNTP in a buffer formulation that has been optimized for first-strand synthesis of cDNA
- The annealing buffer is used in the initial template-primer annealing step
- Separate tubes of oligo(dT) 20 and random hexamers are also provided
- cDNA synthesis can be performed using either total RNA or poly(A) + -selected RNA primed with oligo(dT), random primers, or a gene-specific primer
- Order Info Shipping Condition: Dry ice
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Content And Storage: • 2X Reaction Mix, 500 μL• SuperScript™ III Enzyme Mix, 100 μL• Oligo(dT)20, 50 μL total (50 μM)• Random hexamers, 50 μL (50 ng/μL)• Annealing buffer, 50 μLStore at –20°C.
GC-Rich PCR Performance: High
Technique: Reverse Transcription
Primer: Random Primers, Oligo dT Primers
Shipping Condition: Dry Ice
Final Product Type: First-Strand cDNA
Reaction Format: Master Mix
Starting Material: RNA
Format: Kit
Reaction Speed: 50 min.
Optimal Reaction Temperature: 50°C
Reverse Transcriptase: SuperScript III
For Use With (Application): Real Time PCR (qPCR)
No. of Reactions: 50 Reactions
Reagent Type: Reverse Transcription
Content And Storage:
• 2X Reaction Mix, 500 μL• SuperScript™ III Enzyme Mix, 100 μL• Oligo(dT)20, 50 μL total (50 μM)• Random hexamers, 50 μL (50 ng/μL)• Annealing buffer, 50 μLStore at –20°C.
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription
Primer:
Random Primers, Oligo dT Primers
Shipping Condition:
Dry Ice
Final Product Type:
First-Strand cDNA
Reaction Format:
Master Mix
Starting Material:
RNA
Format:
Kit
Reaction Speed:
50 min.
Optimal Reaction Temperature:
50°C
Reverse Transcriptase:
SuperScript III
For Use With (Application):
Real Time PCR (qPCR)
No. of Reactions:
50 Reactions
Reagent Type:
Reverse Transcription
Content And Storage: • SuperScript IV RT (10 μL)• 5X RT buffer (1 mL)• 0.1 M DTT (500 μL)Store at -15°C to -25°C.
GC-Rich PCR Performance: High
Technique: Reverse Transcription
Primer: __
Shipping Condition: Dry Ice
Final Product Type: First-Strand cDNA
Reaction Format: Separate components
Starting Material: RNA
Format: Stand-alone Enzyme
Reaction Speed: 10 min.
Optimal Reaction Temperature: 50°C
Reverse Transcriptase: SuperScript IV
For Use With (Application): __
No. of Reactions: 10 Reactions
Reagent Type: Reverse Transcription
Content And Storage:
• SuperScript IV RT (10 μL)• 5X RT buffer (1 mL)• 0.1 M DTT (500 μL)Store at -15°C to -25°C.
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription
Primer:
__
Shipping Condition:
Dry Ice
Final Product Type:
First-Strand cDNA
Reaction Format:
Separate components
Starting Material:
RNA
Format:
Stand-alone Enzyme
Reaction Speed:
10 min.
Optimal Reaction Temperature:
50°C
Reverse Transcriptase:
SuperScript IV
For Use With (Application):
__
No. of Reactions:
10 Reactions
Reagent Type:
Reverse Transcription
Content And Storage: • SuperScript IV RT, 50 μL (200 U/μL)• 5X RT buffer, 1 mL• 0.1 M DTT, 500 μLStore at -15°C to –25°C.
GC-Rich PCR Performance: High
Technique: Reverse Transcription
Primer: __
Shipping Condition: Dry Ice
Final Product Type: First-Strand cDNA
Reaction Format: Separate components
Starting Material: RNA
Format: Stand-alone Enzyme
Reaction Speed: 10 min.
Optimal Reaction Temperature: 50°C
Reverse Transcriptase: SuperScript IV
For Use With (Application): __
No. of Reactions: 50 Reactions
Reagent Type: Reverse Transcription
Content And Storage:
• SuperScript IV RT, 50 μL (200 U/μL)• 5X RT buffer, 1 mL• 0.1 M DTT, 500 μLStore at -15°C to –25°C.
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription
Primer:
__
Shipping Condition:
Dry Ice
Final Product Type:
First-Strand cDNA
Reaction Format:
Separate components
Starting Material:
RNA
Format:
Stand-alone Enzyme
Reaction Speed:
10 min.
Optimal Reaction Temperature:
50°C
Reverse Transcriptase:
SuperScript IV
For Use With (Application):
__
No. of Reactions:
50 Reactions
Reagent Type:
Reverse Transcription
Content And Storage: • SuperScript IV RT, 4 x 50 μL (200 U/μL)• 5X RT buffer, 4 x 1000 μL• 0.1 M DTT, 4 x 500 μLStore at -15°C to -25°C.
GC-Rich PCR Performance: High
Technique: Reverse Transcription
Primer: __
Shipping Condition: Dry Ice
Final Product Type: First-Strand cDNA
Reaction Format: Separate components
Starting Material: RNA
Format: Stand-alone Enzyme
Reaction Speed: 10 min.
Optimal Reaction Temperature: 50°C
Reverse Transcriptase: SuperScript IV
For Use With (Application): __
No. of Reactions: 200 Reactions
Reagent Type: Reverse Transcription
Content And Storage:
• SuperScript IV RT, 4 x 50 μL (200 U/μL)• 5X RT buffer, 4 x 1000 μL• 0.1 M DTT, 4 x 500 μLStore at -15°C to -25°C.
GC-Rich PCR Performance:
High
Technique:
Reverse Transcription
Primer:
__
Shipping Condition:
Dry Ice
Final Product Type:
First-Strand cDNA
Reaction Format:
Separate components
Starting Material:
RNA
Format:
Stand-alone Enzyme
Reaction Speed:
10 min.
Optimal Reaction Temperature:
50°C
Reverse Transcriptase:
SuperScript IV
For Use With (Application):
__
No. of Reactions:
200 Reactions
Reagent Type:
Reverse Transcription